Cloned (Comment) | Organism |
---|---|
recombinant expression of C-terminally His6-tagged chimeric allergen R8, derived from the major allergen group 1 of house dust mites species, Dermatophagoides farinae and Dermatophagoides pteronyssinus, in leaves of Nicotiana benthamiana via transfection with Agrobacterium tumefaciens strain GV3101 | Dermatophagoides pteronyssinus |
recombinant expression of C-terminally His6-tagged wild-type enzyme and chimeric allergen R8, derived from the major allergen group 1 of house dust mites species, Dermatophagoides farinae and Dermatophagoides pteronyssinus, in leaves of Nicotiana benthamiana via transfection with Agrobacterium tumefaciens strain GV3101 | Dermatophagoides farinae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a chimeric allergen R8, derived from the major allergen group 1 of house dust mites species, Dermatophagoides farinae and Dermatophagoides pteronyssinus | Dermatophagoides pteronyssinus |
additional information | construction of a chimeric allergen R8, derived from the major allergen group 1 of house dust mites species, Dermatophagoides farinae and Dermatophagoides pteronyssinus | Dermatophagoides farinae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dermatophagoides farinae | Q58A71 | preproenzyme | - |
Dermatophagoides pteronyssinus | P08176 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged chimeric allergen R8 from Nicotiana benthamiana leaves by affinity chromatography to homogeneity under denaturing conditions | Dermatophagoides pteronyssinus |
recombinant His6-tagged wild-type enzyme and chimeric allergen R8 from Nicotiana benthamiana leaves by affinity chromatography to homogeneity under denaturing conditions | Dermatophagoides farinae |
Renatured (Comment) | Organism |
---|---|
recombinant His6-tagged chimeric allergen R8 from Nicotiana benthamiana leaves after purification by affinity chromatography under denaturing conditions, refolding by dialysis against decreasing concentrations of urea | Dermatophagoides pteronyssinus |
recombinant His6-tagged wild-type enzyme and chimeric allergen R8 from Nicotiana benthamiana leaves after purification by affinity chromatography under denaturing conditions, refolding by dialysis against decreasing concentrations of urea | Dermatophagoides farinae |
Synonyms | Comment | Organism |
---|---|---|
ProDer f 1 | - |
Dermatophagoides farinae |
ProDer p 1 | - |
Dermatophagoides pteronyssinus |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme is a group 1 mite allergen | Dermatophagoides pteronyssinus |
additional information | the enzyme is a group 1 mite allergen | Dermatophagoides farinae |