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3.4.22.62: caspase-9

This is an abbreviated version!
For detailed information about caspase-9, go to the full flat file.

Word Map on EC 3.4.22.62

Reaction

strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-/-Xaa =

Synonyms

APAF, Apaf-3, apoptotic protease activating factor 3, apoptotic protease Mch-6, C14.010, CASP-9, casp9, casp9-gamma, caspase 9, caspase-9, ICE-LAP6, ICE-like apoptotic protease 6, Mch6

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.62 caspase-9

Cloned

Cloned on EC 3.4.22.62 - caspase-9

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
adenovirus expression, AdshRNA-C9 and AdshRNA-eGFP vectors, AdBcl-xL and AdNull adenoviral vectors
B6 splenocytes modified by retroviral transduction, truncated caspase-9 molecule genetically coupled to a modified human FK506-binding protein (FKBPF36V) by generating the iCasp9M-construct, iCasp9M construct (safety switch) and enhanced GFP used for transformation
caspase-9 inactivated by mutation at the catalytic domain, generation of dominant negative caspase-9 overexpressing cell lines, expression in Escherichia coli, follicular lymphoma cells transformed with the lentiviral vector pWPI-IRES-GFP
caspase-9 splice variant Casp9-gamma contains only a caspase recruitment domain and lacks the catalytic domain, expression in 293T cells.Casp9-gamma does not promote apoptosis when overexpressed in 293T cells
caspase-9S is cloned from human liver cDNA
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expressed in Escherichia coli
expressed in Escherichia coli, pcmyc vector, pFLAG-CTC vector to generate FLAG-tagged caspase-9 construct
expressed in Escherichia coli, recombinant protein, pcDNA3, pET28a, pEGFP vectors
expression in Escherichia coli
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expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain B21(DE3) in incusion bodies
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gene CSP9, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3)
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gene Lyccasp9, DNA and maino acid sequence determination and analysis, full-length cDNA of Lyccasp9 is 2595 bp with an open reading frame of 1314 bp encoding a polypeptide of 437 amino acids, which includes a 90-amino acids caspase recruitment domain (CARD, residues 1-90), a 133-amino acids p20 domain (residues 171-303) with two putative caspase family histidine and cysteine active sites, as well as an 87-amino acids p10 domain (residues 348-435)
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human caspase-9 (wild type or mutant R10A) are subcloned into the pIND expression plasmid before cotransfection of recombinant pIND and pVgRXR into U2OS cells
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naturally occuring variant caspase-9S, that is missing most of the large subunit of caspase-9
recombinant expression of His6-tagged holoenzyme in Escherichia coli strain BL21(DE3), recombinantindividual expression of large and small subunits in inclusion bodies
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