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Literature summary for 3.4.22.62 extracted from

  • Mu, Y.; Xiao, X.; Zhang, J.; Ao, J.; Chen, X.
    Molecular cloning and functional characterization of caspase 9 in large yellow croaker (Pseudosciaena crocea) (2010), Dev. Comp. Immunol., 34, 300-307.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Lyccasp9, DNA and maino acid sequence determination and analysis, full-length cDNA of Lyccasp9 is 2595 bp with an open reading frame of 1314 bp encoding a polypeptide of 437 amino acids, which includes a 90-amino acids caspase recruitment domain (CARD, residues 1-90), a 133-amino acids p20 domain (residues 171-303) with two putative caspase family histidine and cysteine active sites, as well as an 87-amino acids p10 domain (residues 348-435) Larimichthys crocea

Protein Variants

Protein Variants Comment Organism
C299G site-directed mutagenesis, the mutant displays significantly decreased proteolytic activity compared to the wild-type enzyme Larimichthys crocea
H249D site-directed mutagenesis, the mutant displays significantly decreased proteolytic activity compared to the wild-type enzyme Larimichthys crocea
L228V site-directed mutagenesis Larimichthys crocea

Organism

Organism UniProt Comment Textmining
Larimichthys crocea
-
gene Lyccasp9
-

Source Tissue

Source Tissue Comment Organism Textmining
blood high expression level Larimichthys crocea
-
gill
-
Larimichthys crocea
-
heart high expression level Larimichthys crocea
-
intestine
-
Larimichthys crocea
-
kidney
-
Larimichthys crocea
-
liver
-
Larimichthys crocea
-
additional information Lyccasp9 is constitutively ubiquitously expressed, although expression levels vary from tissue to tissue, real-time PCR expression analysis Larimichthys crocea
-
muscle
-
Larimichthys crocea
-
spleen
-
Larimichthys crocea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-LEDH-4-nitroanilide + H2O
-
Larimichthys crocea Ac-LEDH + 4-nitroaniline
-
?
additional information catalytic Cys299 residue with a His249 involved in catalysis Larimichthys crocea ?
-
?

Subunits

Subunits Comment Organism
More the caspase family signature histidine active-site H236SQYDCCVVIMLSHG250, the cysteine active-site K290PKLFFIQACGG301 and the caspase 9 characteristic pentapeptide active-site QACGG are located in the p20 domain Larimichthys crocea

Synonyms

Synonyms Comment Organism
caspase 9
-
Larimichthys crocea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Larimichthys crocea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Larimichthys crocea

General Information

General Information Comment Organism
evolution multiple sequence alignment and phylogenetic tree show that fish and human caspase 9 are highly evolutionarily conserved Larimichthys crocea
additional information the caspase family signature histidine active-site H236SQYDCCVVIMLSHG250, the cysteine active-site K290PKLFFIQACGG301 and the caspase 9 characteristic pentapeptide active-site QACGG are located in the p20 domain Larimichthys crocea