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Literature summary for 3.4.22.62 extracted from

  • Murray, T.V.; McMahon, J.M.; Howley, B.A.; Stanley, A.; Ritter, T.; Mohr, A.; Zwacka, R.; Fearnhead, H.O.
    A non-apoptotic role for caspase-9 in muscle differentiation (2008), J. Cell Sci., 121, 3786-3793.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
staurosporine initiates apoptosis, 25% of the cells are apoptotic in staurosporine-treated cultures, 1 microM for 4 h, used as a positive control for caspase-9 activation in C2C12 cells, mitochondrial changes in apoptotic but not in differentiating cells Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
adenovirus expression, AdshRNA-C9 and AdshRNA-eGFP vectors, AdBcl-xL and AdNull adenoviral vectors Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
biotin-Val-Ala-Asp-fluoromethyl ketone i.e. biotin-VAD-fmk, presence or absence of 50 microM inhibitor, caspase-9 activation analyzed by affinity labelling and immunoblotting Mus musculus
Q-VD-OPH i.e. (3S)-5-(2,6-difluorophenoxy)-3-[[(2S)-2-(isoquinoline-3-carbonylamino)-3-methylbutanoyl]amino]-4-oxopentanoic acid, cell treatment with the broad-spectrum caspase inhibitor Q-VD-OPH (concentration 30 microM) prior to induction of differentiation Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pro-caspase-3 + H2O Mus musculus requirement of caspase-3 for cell differentiation, activation of caspase-3 by caspase-9 promotes differentiation of skeletal myoblasts into myotubes caspase-3 + ?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus Q9R0T0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
C2C12 cell
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Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
caspase-9 is responsible for the activation of caspase-3 in differentiating myoblasts, decreased caspase-9 levels due to RNA interference prevents caspase-3 activation and impairs muscle differentiation, release of cytochrome c not observed, novel mechanism of caspase-9 activation during muscle differentiation, apoptotic nuclear morphology assessed by DAPI staining, quantification of muscle-cell differentiation, mitochondrial-membrane depolarisation, cell fractionation, immunoblotting Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pro-caspase-3 + H2O requirement of caspase-3 for cell differentiation, activation of caspase-3 by caspase-9 promotes differentiation of skeletal myoblasts into myotubes Mus musculus caspase-3 + ?
-
?