Activating Compound | Comment | Organism | Structure |
---|---|---|---|
staurosporine | initiates apoptosis, 25% of the cells are apoptotic in staurosporine-treated cultures, 1 microM for 4 h, used as a positive control for caspase-9 activation in C2C12 cells, mitochondrial changes in apoptotic but not in differentiating cells | Mus musculus |
Cloned (Comment) | Organism |
---|---|
adenovirus expression, AdshRNA-C9 and AdshRNA-eGFP vectors, AdBcl-xL and AdNull adenoviral vectors | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
biotin-Val-Ala-Asp-fluoromethyl ketone | i.e. biotin-VAD-fmk, presence or absence of 50 microM inhibitor, caspase-9 activation analyzed by affinity labelling and immunoblotting | Mus musculus | |
Q-VD-OPH | i.e. (3S)-5-(2,6-difluorophenoxy)-3-[[(2S)-2-(isoquinoline-3-carbonylamino)-3-methylbutanoyl]amino]-4-oxopentanoic acid, cell treatment with the broad-spectrum caspase inhibitor Q-VD-OPH (concentration 30 microM) prior to induction of differentiation | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-caspase-3 + H2O | Mus musculus | requirement of caspase-3 for cell differentiation, activation of caspase-3 by caspase-9 promotes differentiation of skeletal myoblasts into myotubes | caspase-3 + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9R0T0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
C2C12 cell | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
caspase-9 is responsible for the activation of caspase-3 in differentiating myoblasts, decreased caspase-9 levels due to RNA interference prevents caspase-3 activation and impairs muscle differentiation, release of cytochrome c not observed, novel mechanism of caspase-9 activation during muscle differentiation, apoptotic nuclear morphology assessed by DAPI staining, quantification of muscle-cell differentiation, mitochondrial-membrane depolarisation, cell fractionation, immunoblotting | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-caspase-3 + H2O | requirement of caspase-3 for cell differentiation, activation of caspase-3 by caspase-9 promotes differentiation of skeletal myoblasts into myotubes | Mus musculus | caspase-3 + ? | - |
? |