trienzyme extraction is a combined enzymatic digestion by Pronase, alpha-amylase, and conjugase, i.e. gamma-glutamyl hydrolase, to liberate the carbohydrate and protein-bound folates from food matrices prior to total folate analysis by microbiological assay using Lactobacillus casei ssp. rhamnosus, ATCC 7469, determine the contribution of the enzymes to the growth response, the extraction method is used in AOAC Official Method 2004.05 for the folate analysis of cereal foods
folate analysis. Isoform GGH2 is able to completely deconjugate folates using 50-100 microg/g of sample for 1 h at 37°C in all matrices tested. It removes the tails of folylpolyglutamates from tomato fruit, black bean and peanut seeds, and alfalfa sprouts extracts
gamma-glutamyl hydrolase as biomarkers for pulmonary neuroendocrine tumors by cDNA microarray. Expression of gamma-glutamyl hydrolase correlates with poor prognosis
genoproteomic mining of urothelial cancer suggests gamma-glutamyl hydrolase and diazepam-binding inhibitor as putative urinary markers of outcome after chemotherapy
the relationships between the reduced folate levels in the colorectal cancer tissue after leucovorin administration and the gene-expression levels of folate-metabolizing enzymes and folate transporters is investigated. A multivariate logistic regression analysis reveals that low gamma-glutamyl hydrolase gene expression is a predictive factor for a high reduced folate level after leucovorin administration
overexpression of gamma-glutamyl hydrolase significantly decreases chemosensitivity of MDA-MB-435 cells to 5-fluorouracil and methotrexate at all folate concentrations as expected. In contrast, in HCT116 cells this predicted effect is observed only at very high folate concentration, and as the folate concentration decreases this effect becomes null or paradoxically increases. Inhibition of gamma-glutamyl hydrolase significantly increases chemosensitivity of both cancer cells to 5-fluorouracil at all folate concentrations. gamma-Glutamyl hydrolase inhibition significantly decreases chemosensitivity of both cancer cells to methotrexate at all folate concentrations. In both modulation systems and cell lines, the magnitude of chemosensitivity effect incrementally increases as folate concentration increases