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3.4.19.9: folate gamma-glutamyl hydrolase

This is an abbreviated version!
For detailed information about folate gamma-glutamyl hydrolase, go to the full flat file.

Word Map on EC 3.4.19.9

Reaction

tetrahydropteroyl-(gamma-glutamyl)n
+ (n-1) H2O =
5,6,7,8-tetrahydrofolate
 + (n-1) L-glutamate

Synonyms

At1g78680, AtGGH1, AtGGH2, carboxypeptidase G, conjugase, EC 3.4.12.10, EC 3.4.22.12, FGPH, folate conjugase, folate hydrolase, folic acid conjugase, folylpolyglutamate hydrolase, gamma-GH, gamma-Glu-X carboxypeptidase, gamma-glutamyl hydrolase, gamma-polyglutamic acid hydrolase, gammaGH, GGH, GGH2, hydrolase, gamma-glutamyl, LeGGH1, LeGGH2, LeGGH3, low gamma-glutamyl hydrolase, lysosomal gamma-glutamyl carboxypeptidase, PghP, poly(gamma-glutamic acid) endohydrolase, poly(glutamic acid) hydrolase II, polyglutamate hydrolase, pteroyl-poly-gamma-glutamate hydrolase, pteroylpoly-gamma-glutamyl hydrolase, zgammaGH

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.19 Omega peptidases
                3.4.19.9 folate gamma-glutamyl hydrolase

Cloned

Cloned on EC 3.4.19.9 - folate gamma-glutamyl hydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AtGGH1 is expressed in truncated form in Escherichia coli
AtGGH2 is expressed in truncated form in Escherichia coli
cloned and inserted into a pET43.1a vector via SmaI and EcoRI sites and expressed in Rosetta (DE3) cells as a Nus-His-tag fusion enzyme (NH-zgammaGH)
expressed as a fusion protein containing a C-terminal His-tag in Escherichia coli
-
expression in Escherichia coli
expression of N-terminally His-tagged enzyme in Hi5 insect cells
expression the N-terminally His-tagged and non-tagged glycosylated enzyme in Hi5 insect cells
-
expression with a baculovirus/Sf9 insect cell expression system
-
gene GGH, DNA and amino acid sequence determination and analysis, genotyping, overview
-
gene GGH, DNA and amino acid sequence determination and anaylsis, expression analysis, GGH promoter methylation is not a cause of GGH downregulation in CIMP+ colorectal cancer
GGH activity is directly related to GGH messenger RNA expression in acute lymphoblastic leukemia cells of patients with a wild-type germline GGH genotype, identification of two CpG islands, CpG1 and CpG2, in the region extending from the GGH promoter through the first exon and into intron 1, methylation of both CpG islands in the GGH promoter is associated with significantly reduced GGH mRNA expression and catalytic activity and with significantly higher accumulation of MTX polyglutamates in the cells, methylation of CpG1 is leukemia-cell specific and has a pronounced effect on GGH expression, whereas methylation of CpG2 is common in leukemia cells and normal leukocytes but does not significantly alter GGH expression, expression analysis, overview
-
overexpression in MCF7 breast cancer cells and HT1080 fibrosarcoma cells
-
the gene localizes to chromosome 4 under control of two distinct promoters
-
when coexpressed in Escherichia coli, homodimer is catalytically inactive. LeGGH3 forms heterodimers with LeGGH1 or LeGGH2 that has one-half the activity of the matching homodimer
when coexpressed in Escherichia coli, LeGGH1 forms heterodimers with an intermediate bond cleavage preference
when coexpressed in Escherichia coli, LeGGH2 forms heterodimers with an intermediate bond cleavage preference
wild-type and mutant enymes C19A, C110A, C124A and C290A are expressed in Escherichia coli
-