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3.4.11.22: aminopeptidase I

This is an abbreviated version!
For detailed information about aminopeptidase I, go to the full flat file.

Word Map on EC 3.4.11.22

Reaction

Release of an N-terminal amino acid, preferably a neutral or hydrophobic one, from a polypeptide. Aminoacyl-arylamides are poor substrates =

Synonyms

A-LAP, AAP, Actinase AS, adipocyte-derived leucine amino-peptidase, Aeromonas proteolytica aminopeptidase, alpha-Aminoacyl-peptide hydrolase, Aminooligopeptidase, Aminopeptidase, aminopeptidase (I), aminopeptidase 1, aminopeptidase ERAP1, aminopeptidase I, aminopeptidase II, Aminopeptidase III, aminopeptidase IV, aminopeptidase regulator of TNFR1 shedding, Aminopeptidase yscI, Aminopeptidase yscII, Aminopeptidase yscXVI, Aminopolypeptidase, Amylorhizin aminopeptidase, Amylorizin, AP, APE1, Ape2 aminopeptidase, API, ARTS-1, Bacillus aminopeptidase I, ColAP, cold-active aminopeptidase, endoplasmic resticulum aminopeptidase associated with antigen processing, endoplasmic reticulum aminopeptidase, endoplasmic reticulum aminopeptidase 1, endoplasmic reticulum aminopeptidase associated with antigen presentation, ER aminopeptidase, ER aminopeptidase 1, ER aminopeptidase associated with antigen processing, ERAAP, ERAP1, Jc-peptidase, L-aminopeptidase, LAP, LAP II, LAP IV, LAP4, LAPase, LAPIV, Leu.AP, Leucin aminopeptidase V, leucine aminopeptidase, leucine aminopeptidase II, Leucine aminopeptidase IV, leucine-aminopeptidase, Leucineaminopeptidase I, Leucyl aminopeptidase, Metallo aminopeptidase, More, non-specific monoaminopeptidase, pApe1p, PILS-AP, Pj-peptidase, Polypeptidase, puromycin-insensitive leucyl-specific aminopeptidase, thermophilic aminopeptidase, vacuolar aminopeptidase 1, vacuolar aminopeptidase I, Yeast aminopeptidase I, YKL103C, yscI

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.22 aminopeptidase I

Purification

Purification on EC 3.4.11.22 - aminopeptidase I

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
aminopeptidase yscXVI
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ammonium sulfate precipitation, ion-exchange chromatography and gel filtration
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DEAE-Sephacel column chromatography, ammonium sulfate precipitation, Superdex 200 gel filtration, HiTrap Q column chromatography, and arginine-Sepharose column chromatography
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from culture medium, hydroxyapatite chromatography, immobilized metal ion affinity chromatography (Co2+)
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from culture medium, immobilized metal ion affinity chromatography (Ni2+), gel filtration
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from culture supernatant
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further purification of commercial kidney enzyme preparation by affinity and hydrophobic interaction chromatography
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further purification of the commercial preparation, to homogeneity
glutathione Sepharose 4B column chromatography, Superdex 200 gel filtration, Superdex 75 gel filtration, and Resource RPC column chromatography
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immobilized metal ion affinity chromatography (Ni2+)
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isolation of homogeneous dodecameric enzyme
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native enzyme 178fold to homogeneity by ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, and ion exchange chromatography
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native enzyme 19fold
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native enzyme 460fold to homogeneity by ion exchange and hydroxy apatite chromatography, and ion exchange chromatography
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native enzyme 96fold from pollen by a seven-step procedure comprising ammonium sulfate fractionation, 2 steps of anion exchange chromatography, gel filtration, and hydrophobic interaction chromatography
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native enzyme either 331fold by ammonium sulfate fractionation, heat precipitation, gel filtration, ion exchange and adsorption chromatography, or by a single step anion exchange chromatography process
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native from culture supernatant
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native soluble enzyme from chloroplast stroma to homogeneity by chlorplast preparation, ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, and ion exchange chromatography
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Ni-NTA column chromatography, MonoQ column chromatography, and Superdex S200 gel filtration
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partially
partially, preparation of spores and subcellular fractionation
recombinant His-tagged enzyme from Escherichia coli, 9.2fold to homogeneity
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recombinant wild-type and mutant precursor enzymes
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Sephadex G-200 gel filtration
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