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Literature summary for 3.4.11.22 extracted from

  • Noguchi, Y.; Nagata, H.; Koganei, H.; Kodera, Y.; Hiroto, M.; Nishimura, H.; Inada, Y.; Matsushima, A.
    Isolation and characterization of aminopeptidase (Jc-peptidase) from Japanese cedar pollen (Cryptomeria japonica) (2002), J. Agric. Food Chem., 50, 3540-3543.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
amastatin
-
Cryptomeria japonica
bestatin i.e. [(2S,3R)-3-amino-2-hydroxy-4-phenylbutanoyl-L-valyl]-L-leucine Cryptomeria japonica
additional information leuhistin, arphamenine A and B, and actinonin are poor inhibitors Cryptomeria japonica
phebestin i.e. [(2S,3R)-3-amino-2-hydroxy-4-phenylbutanoyl-L-valyl]-L-phenylalanine Cryptomeria japonica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
1 * 42000, SDS-PAGE Cryptomeria japonica
43000
-
gel filtration Cryptomeria japonica

Organism

Organism UniProt Comment Textmining
Cryptomeria japonica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 96fold from pollen by a seven-step procedure comprising ammonium sulfate fractionation, 2 steps of anion exchange chromatography, gel filtration, and hydrophobic interaction chromatography Cryptomeria japonica

Source Tissue

Source Tissue Comment Organism Textmining
pollen
-
Cryptomeria japonica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.7
-
purified enzyme Cryptomeria japonica

Storage Stability

Storage Stability Organism
4°C, aqueous enzyme solution, in presence of Brij 35, pH 6.0-9.0, stable for a few days Cryptomeria japonica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-7-amido-4-methylcoumarin + H2O
-
Cryptomeria japonica L-Leu + 7-amino-4-methylcoumarin
-
?
L-Met-7-amido-4-methylcoumarin + H2O no activity with Z-Met-7-amido-4-methylcoumarin Cryptomeria japonica L-Met + 7-amino-4-methylcoumarin
-
?
L-Phe-7-amido-4-methylcoumarin + H2O
-
Cryptomeria japonica L-Phe + 7-amino-4-methylcoumarin
-
?
L-Tyr-7-amido-4-methylcoumarin + H2O
-
Cryptomeria japonica L-Tyr + 7-amino-4-methylcoumarin
-
?
additional information substrate specificity, overview, no activity with L-Arg-7-amido-4-methylcoumarin and L-Glu-7-amido-4-methylcoumarin Cryptomeria japonica ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE Cryptomeria japonica

Synonyms

Synonyms Comment Organism
Jc-peptidase
-
Cryptomeria japonica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Cryptomeria japonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
Cryptomeria japonica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.047
-
phebestin pH 7.3, 37°C Cryptomeria japonica
0.11
-
bestatin pH 7.3, 37°C Cryptomeria japonica