Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method with 0.1 M Tris-HCl pH 6.5, 30% polyethylene glycol 400, 0.1 M MgCl2 and 1.1 M NaCl | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vacuole | - |
Saccharomyces cerevisiae | 5773 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
400000 | - |
beyond 400000 Da, gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose 4B column chromatography, Superdex 200 gel filtration, Superdex 75 gel filtration, and Resource RPC column chromatography | Saccharomyces cerevisiae |
Subunits | Comment | Organism |
---|---|---|
dodecamer | x-ray crystallography | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase 1 | - |
Saccharomyces cerevisiae |
APE1 | - |
Saccharomyces cerevisiae |