3.2.1.96: mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase

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For detailed information about mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase, go to the full flat file.

Reaction

Synonyms

acetylglucosaminidase, endo-beta, beta-N-acetylglucosaminidase, CBM32, di-N-acetylchitobiosyl beta-N-acetylglucosaminidase, DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase F1, DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase F2, DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase F3, endo, Endo D, Endo H, Endo M, Endo-A, endo-beta-(1->4)-N-acetylglucosaminidase, endo-beta-1,4-N-acetylglucosamidase, endo-beta-acetylglucosaminidase, endo-beta-GlcNAc-ase, endo-beta-N-acetylglucosaminidase, endo-beta-N-acetylglucosaminidase A, endo-beta-N-acetylglucosaminidase D, endo-beta-N-acetylglucosaminidase F, endo-beta-N-acetylglucosaminidase H, endo-beta-N-acetylglucosaminidase HS, endo-beta-N-acetylglucosaminidase L, endo-beta-N-acetylglucosaminidase M, Endo-BH, endo-BII, Endo-CC1, Endo-CC2, Endo-D, Endo-Fsp, endo-GM, Endo-HO, endo-LE, endo-M, endo-N-acetyl-beta-D-glucosaminidase, endo-N-acetyl-beta-glucosaminidase, Endo-Om, EndoBB, EndoBI-1, EndoBI-2, EndoD, endoE, EndoF1, endoglycosidase F1, endoglycosidase F2, endoglycosidase F3, endoglycosidase S, endohexosaminidase, EndoS2, ENGase, ENGase A, ENGase CI, ENGase CII, ENGase D, ENGase F, ENGase F1, ENGase F2, ENGase F3, ENGase Fsp, ENGase H, ENGase L, ENGase Mx, ENGase PI, ENGase PII, ENGase St, Engase1p, ENGase85A, ENGase85B, mannosyl-glycoprotein 1,4-N-acetamidodeoxy-beta-D-glycohydrolase, Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase, Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F1, Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F2, Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F3, More, murein hydrolase, NAGase

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.96 mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase

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Results	in table
11	Activating Compound
4085	AA Sequence
30	Application
1	CAS Registry Number
31	Cloned(Commentary)
6	Crystallization (Commentary)
684	Disease/ Diagnostics
8	General Information
13	General Stability
7	IC50 Value
58	Inhibitors
11	kcat/KM [mM/s]
3	Ki Value [mM]
40	KM Value [mM]
28	Localization
4	Metals/Ions
57	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
284	Organism
1	Pathway
48	PDB ID
76	pH Optimum
17	pH Range
14	pH Stability
1	pI Value
4	Posttranslational Modification
115	Protein Variants
61	Purification (Commentary)
2	Reaction
1	Reaction Type
166	Reference
1	Renatured (Commentary)
70	Source Tissue
53	Specific Activity [micromol/min/mg]
13	Storage Stability
415	Substrates and Products (Substrate)
43	Subunits
183	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
1	Temperature Range [°C]
26	Temperature Stability [°C]
16	Turnover Number [1/s]

Activating Compound

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Activating Compound on EC 3.2.1.96 - mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2-mercaptoethanol

Elizabethkingia meningoseptica

-

ovalbumin, fetuin, bromelain, ovomucoid, alpha1-acid glycoprotein, immunoglobulin G and influenza virus hemagglutinin are susceptible only after reduction and alkylation or in presence of 1% 2-mercaptoethanol

171696

choline

Streptococcus pneumoniae

-

0.1%, activates

171673

dithiothreitol

2 entries

L-Cys

Aspergillus oryzae

-

1 mM, 18% stimulation

171684

nisin

Staphylococcus simulans

-

activates hydrolysis of cell walls of Micrococcus luteus, no influence of hydrolysis of soluble peptidoglycan

171672

Nonionic detergent

2 entries

-

Pep 5

Staphylococcus simulans

-

cationic peptide antibiotic, activates hydrolysis of cell walls of Micrococcus luteus, no influence of hydrolysis of soluble peptidoglycan

171672

-

poly(L-Lys)

Staphylococcus simulans

-

activates hydrolysis of cell walls of Micrococcus luteus, no influence of hydrolysis of soluble peptidoglycan

171672

Triton X-100

Streptomyces griseus

-

maximal stimulation at 0.03%, or small amounts of other detergents

171695

dithiothreitol

Aspergillus oryzae

-

1 mM, 18% stimulation

171684

dithiothreitol

Homo sapiens

-

optimal activation at 10 mM

171713

Nonionic detergent

Elizabethkingia meningoseptica

-

glycoproteins of retrovirus, lymphocytic choriomeningitis virus

171696

-

Nonionic detergent

Elizabethkingia meningoseptica

-

Pichinde virus, and HLA-A and HLA-B antigens are cleaved in presence of nonionic detergent

171696

-