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3.2.1.96: mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase

This is an abbreviated version!
For detailed information about mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase, go to the full flat file.

Word Map on EC 3.2.1.96

Reaction

(Man)3(GlcNAc)2Asn
+
H2O
=
(Man)3GlcNAc
 +
GlcNAc-Asn

Synonyms

acetylglucosaminidase, endo-beta, beta-N-acetylglucosaminidase, CBM32, di-N-acetylchitobiosyl beta-N-acetylglucosaminidase, DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase F1, DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase F2, DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase F3, endo, Endo D, Endo H, Endo M, Endo-A, endo-beta-(1->4)-N-acetylglucosaminidase, endo-beta-1,4-N-acetylglucosamidase, endo-beta-acetylglucosaminidase, endo-beta-GlcNAc-ase, endo-beta-N-acetylglucosaminidase, endo-beta-N-acetylglucosaminidase A, endo-beta-N-acetylglucosaminidase D, endo-beta-N-acetylglucosaminidase F, endo-beta-N-acetylglucosaminidase H, endo-beta-N-acetylglucosaminidase HS, endo-beta-N-acetylglucosaminidase L, endo-beta-N-acetylglucosaminidase M, Endo-BH, endo-BII, Endo-CC1, Endo-CC2, Endo-D, Endo-Fsp, endo-GM, Endo-HO, endo-LE, endo-M, endo-N-acetyl-beta-D-glucosaminidase, endo-N-acetyl-beta-glucosaminidase, Endo-Om, EndoBB, EndoBI-1, EndoBI-2, EndoD, endoE, EndoF1, endoglycosidase F1, endoglycosidase F2, endoglycosidase F3, endoglycosidase S, endohexosaminidase, EndoS2, ENGase, ENGase A, ENGase CI, ENGase CII, ENGase D, ENGase F, ENGase F1, ENGase F2, ENGase F3, ENGase Fsp, ENGase H, ENGase L, ENGase Mx, ENGase PI, ENGase PII, ENGase St, Engase1p, ENGase85A, ENGase85B, mannosyl-glycoprotein 1,4-N-acetamidodeoxy-beta-D-glycohydrolase, Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase, Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F1, Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F2, Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F3, More, murein hydrolase, NAGase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.96 mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase

Cloned

Cloned on EC 3.2.1.96 - mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed from vector pET41-b in Escherichia coli BL21 (DE3)
-
expressed in a silkworm-baculovirus expression system
-
expressed in Candida boidinii
-
expressed in COS-7 cells
expressed in Escherichia coli
expressed in Escherichia coli B834 (DE3) cells
expressed in Escherichia coli BL21 pLysS cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Ogataea minuta
expressed in Saccharomyces cerevisiae png1DELTA cells
-
expression in Escherichia coli
expression in Escherichia coli BL21-CodonPlus (DE3)
expression in Escherichia coli with 150fold improvement in yield
-
expression in Escherichia coli, carbohydrate-binding module fused to the N-terminus of mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase. The fusion protein also carries a hexahistidine tag
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), and of selenomethionine-labeled enzymes in strain B834(DE3)
expression of wild-type Endo-M and mutants in Escherichia coli strain BL21(DE3)
-
into vector pET15b and transformed into Escherichia coli BL21(DE3) cells
overexpression in Escherichia coli TOP10, enzymes with truncation or point mutation
-
pGEX-2T/Endo-A plasmid overexpressed in Escherichia coli
-
silkworm-baculovirus expression system