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3.2.1.96: mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase

This is an abbreviated version!
For detailed information about mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase, go to the full flat file.

Word Map on EC 3.2.1.96

Reaction

(Man)3(GlcNAc)2Asn
+
H2O
=
(Man)3GlcNAc
+
GlcNAc-Asn

Synonyms

acetylglucosaminidase, endo-beta, beta-N-acetylglucosaminidase, CBM32, di-N-acetylchitobiosyl beta-N-acetylglucosaminidase, DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase F1, DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase F2, DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase F3, endo, Endo D, Endo H, Endo M, Endo-A, endo-beta-(1->4)-N-acetylglucosaminidase, endo-beta-1,4-N-acetylglucosamidase, endo-beta-acetylglucosaminidase, endo-beta-GlcNAc-ase, endo-beta-N-acetylglucosaminidase, endo-beta-N-acetylglucosaminidase A, endo-beta-N-acetylglucosaminidase D, endo-beta-N-acetylglucosaminidase F, endo-beta-N-acetylglucosaminidase H, endo-beta-N-acetylglucosaminidase HS, endo-beta-N-acetylglucosaminidase L, endo-beta-N-acetylglucosaminidase M, Endo-BH, endo-BII, Endo-CC1, Endo-CC2, Endo-D, Endo-Fsp, endo-GM, Endo-HO, endo-LE, endo-M, endo-N-acetyl-beta-D-glucosaminidase, endo-N-acetyl-beta-glucosaminidase, Endo-Om, EndoBB, EndoBI-1, EndoBI-2, EndoD, endoE, EndoF1, endoglycosidase F1, endoglycosidase F2, endoglycosidase F3, endoglycosidase S, endohexosaminidase, EndoS2, ENGase, ENGase A, ENGase CI, ENGase CII, ENGase D, ENGase F, ENGase F1, ENGase F2, ENGase F3, ENGase Fsp, ENGase H, ENGase L, ENGase Mx, ENGase PI, ENGase PII, ENGase St, Engase1p, ENGase85A, ENGase85B, mannosyl-glycoprotein 1,4-N-acetamidodeoxy-beta-D-glycohydrolase, Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase, Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F1, Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F2, Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F3, More, murein hydrolase, NAGase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.96 mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase

General Stability

General Stability on EC 3.2.1.96 - mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
20-30% loss of initial activity is lost when the protein concentration is decreased to less than 0.02-0.03 mg/ml
-
activity decreases on repeated freezing
-
pronase, trypsin, and chymotrypsin at 2.5 mg per ml, when incubated with 0.005-0.010 mg of enzyme per ml, has little or no effect after 5 h at 37°C. 5 mg/ml pronase inactivates slowly during a 12 h period
-
retains nearly 30% of its activity in 1% SDS
-
stable to freezing at -70°C
-
stable to incubation with 250fold excess of either Pronase, chymotrypsin, or trypsin w/w. Slow inactivation by 1000fold excess of Pronase during 12 h incubation
-
stable to lyophilization
stable to repeated freezing and thawing
-
stable to repeated freezing and thawing or lyophilization
-
trypsin, 0.01 mg/ml, pH 7.2, 50 mM Tris/HCl, 70% inactivation after 15 min
-