3.2.1.75: glucan endo-1,6-beta-glucosidase
This is an abbreviated version!
For detailed information about glucan endo-1,6-beta-glucosidase, go to the full flat file.
Word Map on EC 3.2.1.75
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3.2.1.75
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trichoderma
-
harzianum
-
mycoparasitic
-
beta-1,3-glucanase
-
biocontrol
-
gentiobiose
-
laminarin
-
beta-glucanases
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virens
-
neotyphodium
-
chitinases
-
wall-degrading
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biotechnology
-
rhizoctonia
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analysis
- 3.2.1.75
- trichoderma
- harzianum
-
mycoparasitic
- beta-1,3-glucanase
-
biocontrol
- gentiobiose
- laminarin
- beta-glucanases
- virens
- neotyphodium
- chitinases
-
wall-degrading
- biotechnology
-
rhizoctonia
- analysis
Reaction
Synonyms
acidic beta-1,6-glucanase, beta-(1->6)-glucanase, beta-1, 6-endoglucanase, beta-1,6-endoglucanase, beta-1,6-glucan 6-glucanohydrolase, beta-1,6-glucan hydrolase, beta-1,6-glucanase, beta-1,6-glucanase-pustulanase, beta-1-6-glucan hydrolase, BGN16.3, BT3312, endo-(1-6)-beta-D-glucanase, endo-1,6-beta-D-glucanase gene, endo-1,6-beta-glucanase, endo-beta-(1,6) glucanase, endo-beta-1,6-glucanase, gly30B, neg-1, Neg1, PUL1,6-beta-glucan, Pus30A
ECTree
3.2.1.75 glucan endo-1,6-beta-glucosidaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.2.1.75 - glucan endo-1,6-beta-glucosidase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
beta-(1,6)-glucan + H2O
?
-
highly branched beta-(1,6)-glucan, that contains about 75-80% of linear beta-(1,6)-glucose linked units, preared from yeast cell walls
-
-
?
Eisenia bicyclis laminarin + H2O
?
27% of the activity with pustulan
-
-
?
gentiopentaosylsorbitol + H2O
gentiotetraose + gentiobiose + D-glucose
-
-
-
?
methyl beta-gentiobioside + H2O
methyl beta-D-glucoside + beta-D-glucose
-
low activity
-
-
?
methyl beta-gentiohexaoside + H2O
methyl beta-D-glucoside + 2 beta-D-glucose + gentiobiose
-
the major cleavages occur at bonds 2, 3, and 4 in approximately equal proportion, the nonreducing end glucosyl residues of Gen4-6 beta-OMe are either not or scarcely cleaved by the enzyme
-
-
?
methyl beta-gentiopentaoside + H2O
methyl beta-D-glucoside + 3 beta-D-glucose
-
the major cleavages occur at bonds 2, 3, and 4 in approximately equal proportion
-
-
?
methyl beta-gentiotetraoside + H2O
methyl beta-D-glucoside + 2 beta-D-glucose
-
cleavage at the second (bond 2) and third (bond 3) glucosidic bonds from the methyl group
-
-
?
methyl beta-gentiotrioside + H2O
methyl beta-D-glucoside + gentiobiose
-
the second glucosidic bond (bond 2) from the methyl group is cut preferentially
Glc beta-OMe and Gen2 beta-OMe in the ratio 91:9
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
-
yeast glucans
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
-
yeast glucans
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
-
yeast glucans
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
-
Neurospora crassa cell wall beta-glucan
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
-
yeast glucans
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
Niallia circulans WL-12
-
yeast glucans
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
-
Ecklonia radiata glucan, poor substrate
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
-
Ecklonia radiata glucan, poor substrate
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
-
yeast glucans
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
-
Saccharomyces cerevisiae glucan
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
-
Saccharomyces cerevisiae glucan
-
-
?
gentiohexaose + H2O
gentiobiose + gentiotriose + gentiotetraose
-
-
-
?
gentiopentaose + H2O
D-glucose + gentiotetraose + gentiotriose + gentiobiose
-
reduced
-
?
gentiopentaose + H2O
D-glucose + gentiotetraose + gentiotriose + gentiobiose
-
-
-
-
?
gentiopentaose + H2O
D-glucose + gentiotetraose + gentiotriose + gentiobiose
-
-
gentiobiose and gentiotriose only
?
gentiopentaose + H2O
D-glucose + gentiotetraose + gentiotriose + gentiobiose
-
-
gentiobiose and gentiotriose only
?
gentiotetraose + H2O
D-glucose + gentiotriose + gentiobiose
-
reduced
-
?
gentiotetraose + H2O
D-glucose + gentiotriose + gentiobiose
-
-
gentiobiose only
?
gentiotetraose + H2O
D-glucose + gentiotriose + gentiobiose
-
-
gentiobiose only
?
glucan + H2O
?
-
substrate from yeast, 18% of maximal activity
-
-
?
laminarin + H2O
D-glucose + ?
3% activity compared to pustulan
the main product is D-glucose
-
?
laminarin + H2O
D-glucose + ?
3% activity compared to pustulan
the main product is D-glucose
-
?
laminarin + H2O
gentobiose + D-glucose
22.4% activity compared to pustulan
-
-
?
luteose + H2O
gentiobiose + gentiotriose + gentiotetraose
-
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
Flavobacterium M64
-
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
?
pustulan + H2O
?
high substrate specificity, 100% activity
-
-
?
pustulan + H2O
?
-
the enzyme completely hydrolyzes pustulan in three steps: in the initial stage, the enzyme quickly cleaves the glucan with a pattern resembling an endo-hydrolase to produce a short-chain glucan (degree of polymerization DP 45) as an intermediate. In the midterm stage, the resulting short-chain glucan is further cleaved into two fractions corresponding to DP 15-7 and DP 2-4 with great regularity. In the final stage, the lower oligomers corresponding to DP 3 and DP 4 are very slowly hydrolyzed into glucose and gentiobiose
-
-
?
pustulan + H2O
?
-
the enzyme completely hydrolyzes pustulan in three steps: in the initial stage, the enzyme quickly cleaves the glucan with a pattern resembling an endo-hydrolase to produce a short-chain glucan (degree of polymerization DP 45) as an intermediate. In the midterm stage, the resulting short-chain glucan is further cleaved into two fractions corresponding to DP 15-7 and DP 2-4 with great regularity. In the final stage, the lower oligomers corresponding to DP 3 and DP 4 are very slowly hydrolyzed into glucose and gentiobiose
-
-
?
pustulan + H2O
gentobiose + D-glucose
highly specific substrate
-
-
?
pustulan + H2O
gentobiose + D-glucose
highly specific substrate
-
-
?
pustulan + H2O
glucose + gentiobiose
-
-
glucose and oligosaccharides
?
pustulan + H2O
glucose + gentiobiose
-
-
glucose, gentiobiose and gentiotriose
?
pustulan + H2O
glucose + gentiobiose
-
-
also gentiopentaose, gentiotetraose and gentiotriose
?
pustulan + H2O
glucose + gentiobiose
Niallia circulans WL-12
-
-
also gentiopentaose, gentiotetraose and gentiotriose
?
pustulan + H2O
glucose + gentiobiose
-
-
gentiotetraose and longer chains, gentiotriose, gentiotriose and glucose
?
pustulan + H2O
glucose + gentiobiose
-
-
glucose, gentiotetraose, gentiobiose
?
pustulan + H2O
glucose + gentiobiose
-
-
glucose, gentiotetraose, gentiobiose
?
pustulan + H2O
oligomers of pustulan
-
best substrate
product analysis, most abundant products are di-, tri-, and tetra-beta-1,6-glucosides
-
?
pustulan + H2O
oligomers of pustulan
-
best substrate
product analysis, most abundant products are di-, tri-, and tetra-beta-1,6-glucosides
-
?
starch + H2O
?
-
soluble, from corn, from potato, from wheat
-
-
?
starch + H2O
?
-
soluble, from corn, from potato, from wheat
-
-
?
additional information
?
-
-
is active on Candida albicans cell walls, is able to release a GPI-anchored cell wall protein linked to the beta-1,6-glucan polymer through a remnant of its anchor. No activity with beta-1,3-glucan, dextran and amylose
-
-
?
additional information
?
-
the enzyme shows no activity on gentiobiose, 1,3-beta-glucan, 1,4-beta-glucan, or 1,6-beta-galactan
-
-
?
additional information
?
-
-
the enzyme shows no activity on gentiobiose, 1,3-beta-glucan, 1,4-beta-glucan, or 1,6-beta-galactan
-
-
?
additional information
?
-
the enzyme shows no activity on gentiobiose, 1,3-beta-glucan, 1,4-beta-glucan, or 1,6-beta-galactan
-
-
?
additional information
?
-
no activity is detected when gentiobiose, lentinan, laminarioligosaccharides, pachyman, CM-pachyman, and lichenan are used as substrates
-
-
?
additional information
?
-
the purified endo-beta-1,6-glucanase Pus30A catalyzes depolymerization of beta-1,6-glucan endolytically and is highly specific toward beta-1,6-glucan polysaccharide. Pus30A shows hydrolyzing activity against the cell wall components of Lentinula edodes fruiting bodies (54.5% activity compared to pustulan)
-
-
?
additional information
?
-
no activity is detected when gentiobiose, lentinan, laminarioligosaccharides, pachyman, CM-pachyman, and lichenan are used as substrates
-
-
?
additional information
?
-
the purified endo-beta-1,6-glucanase Pus30A catalyzes depolymerization of beta-1,6-glucan endolytically and is highly specific toward beta-1,6-glucan polysaccharide. Pus30A shows hydrolyzing activity against the cell wall components of Lentinula edodes fruiting bodies (54.5% activity compared to pustulan)
-
-
?
additional information
?
-
-
the enzyme is a specific beta-(1->6)-glucanase, it shows no activity with soluble starch, carboxymethyl cellulose, curdlan from Alcaligenes faecalis, and 4-nitrophenyl beta-gentiobioside, it shows poor activity with yeast glucan and 4-nitrophenyl beta-gentiotrioside. The cleavage point of 4-nitrophenyl oligosacchride derivatives with DP 4 or higher shifts such that the enzyme keeps away from the 4-nitrophenyl group at the terminus, in all the tested substrates, release of 4-nitrophenol is not observed. Gentiooligosaccharides (DP 3-9) are prepared from the starting substance gentiobiose by the sequential transglycosylation reactions of beta-glucosidase and beta-(1->6)-glucanase
-
-
?
additional information
?
-
-
the enzyme is a specific beta-(1->6)-glucanase, it shows no activity with soluble starch, carboxymethyl cellulose, curdlan from Alcaligenes faecalis, and 4-nitrophenyl beta-gentiobioside, it shows poor activity with yeast glucan and 4-nitrophenyl beta-gentiotrioside. The cleavage point of 4-nitrophenyl oligosacchride derivatives with DP 4 or higher shifts such that the enzyme keeps away from the 4-nitrophenyl group at the terminus, in all the tested substrates, release of 4-nitrophenol is not observed. Gentiooligosaccharides (DP 3-9) are prepared from the starting substance gentiobiose by the sequential transglycosylation reactions of beta-glucosidase and beta-(1->6)-glucanase
-
-
?
additional information
?
-
no activity with curdlan, barley beta-glucan, xylan and carboxymethyl cellulose
-
-
?
additional information
?
-
-
no activity with curdlan, barley beta-glucan, xylan and carboxymethyl cellulose
-
-
?
additional information
?
-
-
little activity against beta-(1-3)- or beta-(1-4)-linkages
-
-
?
additional information
?
-
-
BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview
-
-
?
additional information
?
-
-
substrate specificity, BGN16.3 shows endohydrolytic activity against substrates with beta-1,6-glycosidic linkages, no activity with colloidal chitin, pachyman, starch, cellulose, nigeran, and dextran, no activity with the beta-1,6-disaccharide gentiobiose
-
-
?
additional information
?
-
-
no activity with laminarin, chitin, and mannan
-
-
?
additional information
?
-
-
BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview
-
-
?
additional information
?
-
-
substrate specificity, BGN16.3 shows endohydrolytic activity against substrates with beta-1,6-glycosidic linkages, no activity with colloidal chitin, pachyman, starch, cellulose, nigeran, and dextran, no activity with the beta-1,6-disaccharide gentiobiose
-
-
?
