Information on EC 3.2.1.75 - glucan endo-1,6-beta-glucosidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.2.1.75
-
RECOMMENDED NAME
GeneOntology No.
glucan endo-1,6-beta-glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Random hydrolysis of (1->6)-linkages in (1->6)-beta-D-glucans
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
6-beta-D-glucan glucanohydrolase
Acts on lutean, pustulan and 1,6-oligo-beta-D-glucosides.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
acidic beta-1,6-glucanase
-
-
acidic beta-1,6-glucanase
Hypocrea lixii CECT 2413
-
-
-
beta-1, 6-endoglucanase
-
-
beta-1,6-glucan 6-glucanohydrolase
-
-
-
-
beta-1,6-glucan hydrolase
-
-
-
-
beta-1,6-glucanase
-
-
beta-1,6-glucanase-pustulanase
-
-
-
-
beta-1-6-glucan hydrolase
-
-
-
-
BGN16.3
-
-
BGN16.3
Hypocrea lixii CECT 2413
-
-
-
endo-(1-6)-beta-D-glucanase
-
-
-
-
endo-1,6-beta-D-glucanase gene
Q7M4T0
-
endo-1,6-beta-glucanase
-
-
-
-
endo-beta-1,6-glucanase
-
-
-
-
endo-beta-1,6-glucanase
F8WPI4
-
endo-beta-1,6-glucanase
Lentinula edodes H 600
F8WPI4
-
-
neg-1
Q7M4T0
-
Pus30A
F8WPI4
-
Pus30A
Lentinula edodes H 600
F8WPI4
-
-
CAS REGISTRY NUMBER
COMMENTARY
37278-39-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
IMI 383068, growth on pustulan as sole carbon source
Swissprot
Manually annotated by BRENDA team
IMI 383068, growth on scleroglucan or pustulan as sole carbon source
-
-
Manually annotated by BRENDA team
4 different species
-
-
Manually annotated by BRENDA team
Bacillus circulans WL-12
WL-12
-
-
Manually annotated by BRENDA team
Flavobacterium M64
-
-
-
Manually annotated by BRENDA team
Fusarium roseum
-
-
-
Manually annotated by BRENDA team
Helminthosporium sesanum
-
-
-
Manually annotated by BRENDA team
CECT 2313
-
-
Manually annotated by BRENDA team
strain CECT 2413, 3 isozymes BGN16.1, BGN16.2, and BGN16.3
-
-
Manually annotated by BRENDA team
Hypocrea lixii CECT 2313
CECT 2313
-
-
Manually annotated by BRENDA team
Hypocrea lixii CECT 2413
strain CECT 2413, 3 isozymes BGN16.1, BGN16.2, and BGN16.3
-
-
Manually annotated by BRENDA team
Lentinula edodes H 600
-
UniProt
Manually annotated by BRENDA team
Neotyphodium sp.
-
SwissProt
Manually annotated by BRENDA team
strain IFO-6068
-
-
Manually annotated by BRENDA team
Neurospora crassa IFO-6068
strain IFO-6068
-
-
Manually annotated by BRENDA team
Penicillium brefeldianum QM 1873
QM 1873
-
-
Manually annotated by BRENDA team
10 different species
-
-
Manually annotated by BRENDA team
Rhizopus microsporus var. chinensis R-69
R-69
-
-
Manually annotated by BRENDA team
Saccharomycopsis fibuligera NCYC 451
NCYC 451
-
-
Manually annotated by BRENDA team
growth medium with lyophilized cells of Candida utilis as mian nutrient source
-
-
Manually annotated by BRENDA team
Trichoderma sp.
several strains: A.11.178, A.9.3., B.1.2, T.16.C, T.2.C
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
F8WPI4
endo-beta-1,6-glucanase Pus30A is responsible for the degradation of the cell wall components during fruiting body autolysis after harvest
physiological function
Lentinula edodes H 600
-
endo-beta-1,6-glucanase Pus30A is responsible for the degradation of the cell wall components during fruiting body autolysis after harvest
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
amylopectin + H2O
?
show the reaction diagram
-
-
-
-
?
amylose + H2O
?
show the reaction diagram
-
-
-
-
?
amylose + H2O
?
show the reaction diagram
Rhizopus microsporus var. chinensis, Rhizopus microsporus var. chinensis R-69
-
-
-
-
?
amylose + H2O
?
show the reaction diagram
Neurospora crassa IFO-6068
-
-
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
show the reaction diagram
-
Neurospora crassa cell wall beta-glucan
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
show the reaction diagram
-
yeast glucans
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
show the reaction diagram
-
yeast glucans
-
-
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
show the reaction diagram
-
yeast glucans
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
show the reaction diagram
-
yeast glucans
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
show the reaction diagram
-
yeast glucans
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
show the reaction diagram
-
Saccharomyces cerevisiae glucan
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
show the reaction diagram
-
Ecklonia radiata glucan, poor substrate
-
-
-
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
show the reaction diagram
Bacillus circulans WL-12
-
yeast glucans
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
show the reaction diagram
Hypocrea lixii CECT 2313
-
Saccharomyces cerevisiae glucan
-
-
?
beta-glucan + H2O
glucose + laminari-oligosaccharides + gentio-oligosaccharides
show the reaction diagram
Penicillium brefeldianum QM 1873
-
Ecklonia radiata glucan, poor substrate
-
-
-
carboxymethyl cellulose + H2O
?
show the reaction diagram
Flavobacterium M64
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl-pachyman + H2O
?
show the reaction diagram
Penicillium brefeldianum, Penicillium brefeldianum QM 1873
-
poor substrate
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Rhizopus microsporus var. chinensis, Rhizopus microsporus var. chinensis R-69
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Neurospora crassa IFO-6068
-
-
-
-
?
chitosan + H2O
?
show the reaction diagram
-
3% of the activity with pustulan
-
-
?
curdlan + H2O
?
show the reaction diagram
-
0.3% of the activity against pustulan
-
-
?
dextran + H2O
?
show the reaction diagram
-
-
-
-
?
dextran + H2O
?
show the reaction diagram
Rhizopus microsporus var. chinensis, Rhizopus microsporus var. chinensis R-69
-
low molecular weight
-
-
?
Eisenia bicyclis laminarin + H2O
?
show the reaction diagram
P82288
27% of the activity with pustulan
-
-
?
gentiobiose + H2O
D-glucose
show the reaction diagram
Flavobacterium M64
-
-
-
?
gentiobiose + H2O
D-glucose
show the reaction diagram
Penicillium brefeldianum, Penicillium brefeldianum QM 1873
-
poor substrate
-
?
gentiohexaose + H2O
gentiobiose + gentiotriose + gentiotetraose
show the reaction diagram
-
-
-
-
?
gentiohexaose + H2O
gentiobiose + gentiotriose + gentiotetraose
show the reaction diagram
-
-
-
?
gentiopentaose + H2O
D-glucose + gentiotetraose + gentiotriose + gentiobiose
show the reaction diagram
-
-
-
-
?
gentiopentaose + H2O
D-glucose + gentiotetraose + gentiotriose + gentiobiose
show the reaction diagram
-
-
gentiobiose and gentiotriose only
?
gentiopentaose + H2O
D-glucose + gentiotetraose + gentiotriose + gentiobiose
show the reaction diagram
-
-
gentiobiose and gentiotriose only
?
gentiopentaose + H2O
D-glucose + gentiotetraose + gentiotriose + gentiobiose
show the reaction diagram
-
reduced
-
?
gentiopentaosylsorbitol + H2O
gentiotetraose + gentiobiose + D-glucose
show the reaction diagram
-
-
-
?
gentiotetraose + H2O
D-glucose + gentiotriose + gentiobiose
show the reaction diagram
-
-
-
-
?
gentiotetraose + H2O
D-glucose + gentiotriose + gentiobiose
show the reaction diagram
-
-
-
-
?
gentiotetraose + H2O
D-glucose + gentiotriose + gentiobiose
show the reaction diagram
-
-
gentiobiose only
?
gentiotetraose + H2O
D-glucose + gentiotriose + gentiobiose
show the reaction diagram
-
-
gentiobiose only
?
gentiotetraose + H2O
D-glucose + gentiotriose + gentiobiose
show the reaction diagram
-
reduced
-
?
gentiotetraose + H2O
D-glucose + gentiotriose + gentiobiose
show the reaction diagram
Bacillus circulans WL-12
-
-
-
-
?
gentiotriose + H2O
D-glucose + gentiobiose
show the reaction diagram
-
-
-
?
gentiotriosylsorbitol + H2O
?
show the reaction diagram
-
-
-
-
?
glucan + H2O
?
show the reaction diagram
Hypocrea lixii, Hypocrea lixii CECT 2413
-
substrate from yeast, 18% of maximal activity
-
-
?
glycogen + H2O
?
show the reaction diagram
-
-
-
-
?
glycogen + H2O
?
show the reaction diagram
Rhizopus microsporus var. chinensis, Rhizopus microsporus var. chinensis R-69
-
-
-
-
?
glycogen + H2O
?
show the reaction diagram
Neurospora crassa IFO-6068
-
-
-
-
?
gyrophoran + H2O
?
show the reaction diagram
-
-
-
-
?
islandican + H2O
?
show the reaction diagram
-
-
-
-
?
lactose + H2O
D-glucose
show the reaction diagram
-
-
-
?
laminaran + H2O
?
show the reaction diagram
-
-
-
-
?
laminaran + H2O
?
show the reaction diagram
-
-
-
-
?
laminaran + H2O
?
show the reaction diagram
-
from Eisenia bicyclis
-
-
?
laminarin + H2O
?
show the reaction diagram
-
-
-
-
?
laminarin + H2O
?
show the reaction diagram
-
0.5% of the activity against pustulan
-
-
?
laminarin + H2O
?
show the reaction diagram
Neotyphodium sp.
Q8NJN4
12% of the activity with pustulan
-
-
-
laminarin + H2O
?
show the reaction diagram
-
8% of maximal activity
-
-
?
laminarin + H2O
?
show the reaction diagram
-
only 2% of activity at 45C
-
-
?
laminarin + H2O
?
show the reaction diagram
Hypocrea lixii CECT 2413
-
8% of maximal activity
-
-
?
laminarin + H2O
?
show the reaction diagram
Q8NJN4
12% of the activity with pustulan
-
-
-
laminarin + H2O
gentiobiose + D-glucose
show the reaction diagram
-
-
-
?
laminarin + H2O
gentiobiose + D-glucose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
gentiobiose + D-glucose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
gentiobiose + D-glucose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
gentiobiose + D-glucose
show the reaction diagram
-
-
-
-
?
laminarin + H2O
gentiobiose + D-glucose
show the reaction diagram
Saccharomycopsis fibuligera, Saccharomycopsis fibuligera NCYC 451
-
-
-
-
?
laminarin + H2O
gentiobiose + D-glucose
show the reaction diagram
Hypocrea lixii CECT 2313
-
-
-
-
?
laminarin + H2O
D-glucose + ?
show the reaction diagram
Lentinula edodes, Lentinula edodes H 600
F8WPI4
3% activity compared to pustulan
the main product is D-glucose
-
?
lichenan + H2O
?
show the reaction diagram
Neurospora crassa, Neurospora crassa IFO-6068
-
-
-
-
?
lutean + H2O
D-glucose + gentiobiose + gentiotriose
show the reaction diagram
-
-
-
?
lutean + H2O
D-glucose + gentiobiose + gentiotriose
show the reaction diagram
-
-
-
-
?
lutean + H2O
D-glucose + gentiobiose + gentiotriose
show the reaction diagram
-
-
-
?
lutean + H2O
D-glucose + gentiobiose + gentiotriose
show the reaction diagram
-
-
-
-
?
lutean + H2O
D-glucose + gentiobiose + gentiotriose
show the reaction diagram
-
-
-
?
lutean + H2O
D-glucose + gentiobiose + gentiotriose
show the reaction diagram
Gibberella fujikuroi, Helminthosporium sesanum, Alternaria bataticola
-
-
-
?
lutean + H2O
?
show the reaction diagram
P82288
73% of the activity with pustulan
-
-
?
luteose + H2O
gentiobiose + gentiotriose + gentiotetraose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
Flavobacterium M64
-
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
Bacillus circulans WL-12
-
-
-
?
pachyman + H2O
gentiobiose
show the reaction diagram
-
-
-
-
?
pachyman + H2O
gentiobiose
show the reaction diagram
-
poor substrate
-
-
?
pachyman + H2O
gentiobiose
show the reaction diagram
Bacillus circulans, Bacillus circulans WL-12
-
poor substrate
trace
?
pachyman + H2O
gentiobiose
show the reaction diagram
Penicillium brefeldianum QM 1873
-
poor substrate
-
-
?
pustulan + H2O
?
show the reaction diagram
-
-
-
-
?
pustulan + H2O
?
show the reaction diagram
P82288
-
-
-
?
pustulan + H2O
?
show the reaction diagram
Neotyphodium sp.
Q8NJN4
-
-
-
?
pustulan + H2O
?
show the reaction diagram
Lentinula edodes, Lentinula edodes H 600
F8WPI4
high substrate specificity, 100% activity
-
-
?
pustulan + H2O
?
show the reaction diagram
Q8NJN4
-
-
-
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
-
-
-
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
-
-
-
-
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
-
-
-
-
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
-
-
-
-
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
Trichoderma sp.
-
-
-
-
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
-
-
-
-
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
-
-
also gentiopentaose, gentiotetraose and gentiotriose
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
-
-
glucose, gentiobiose and gentiotriose
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
-
-
gentiotetraose and longer chains, gentiotriose, gentiotriose and glucose
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
-
-
glucose, gentiotetraose, gentiobiose
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
-
-
glucose and oligosaccharides
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
Neurospora crassa IFO-6068
-
-
-
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
Bacillus circulans WL-12
-
-
also gentiopentaose, gentiotetraose and gentiotriose
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
Bacillus circulans WL-12
-
-
-
-
?
pustulan + H2O
glucose + gentiobiose
show the reaction diagram
Hypocrea lixii CECT 2313
-
-
glucose, gentiotetraose, gentiobiose
?
pustulan + H2O
D-glucose + gentobiose + ?
show the reaction diagram
-
-
main products
-
?
pustulan + H2O
gentobiose + D-glucose + ?
show the reaction diagram
-
-
-
-
?
pustulan + H2O
oligomers of pustulan
show the reaction diagram
Hypocrea lixii, Hypocrea lixii CECT 2413
-
best substrate
product analysis, most abundant products are di-, tri-, and tetra-beta-1,6-glucosides
-
?
starch + H2O
?
show the reaction diagram
-
soluble, from corn, from potato, from wheat
-
-
?
starch + H2O
?
show the reaction diagram
-
8% of the activity with pustulan
-
-
?
starch + H2O
?
show the reaction diagram
Rhizopus microsporus var. chinensis R-69
-
soluble, from corn, from potato, from wheat
-
-
?
succinoglycan + H2O
?
show the reaction diagram
Flavobacterium M64
-
-
-
-
?
xylan + H2O
?
show the reaction diagram
-
12% of the activity with pustulan
-
-
?
luteose + H2O
gentiobiose + gentiotriose + gentiotetraose
show the reaction diagram
Flavobacterium M64
-
-
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
little activity against beta-(1-3)- or beta-(1-4)-linkages
-
-
-
additional information
?
-
-
no substrate: laminarin
-
-
-
additional information
?
-
-
BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview, substrate specificity, BGN16.3 shows endohydrolytic activity against substrates with beta-1,6-glycosidic linkages, no activity with colloidal chitin, pachyman, starch, cellulose, nigeran, and dextran, no activity with the beta-1,6-disaccharide gentiobiose
-
-
-
additional information
?
-
-
is active on Candida albicans cell walls, is able to release a GPI-anchored cell wall protein linked to the beta-1,6-glucan polymer through a remnant of its anchor. No activity with beta-1,3-glucan, dextran and amylose
-
-
-
additional information
?
-
F8WPI4
no activity is detected when gentiobiose, lentinan, laminarioligosaccharides, pachyman, CM-pachyman, and lichenan are used as substrates, the purified endo-beta-1,6-glucanase Pus30A catalyzes depolymerization of beta-1,6-glucan endolytically and is highly specific toward beta-1,6-glucan polysaccharide. Pus30A shows hydrolyzing activity against the cell wall components of Lentinula edodes fruiting bodies (54.5% activity compared to pustulan)
-
-
-
additional information
?
-
Lentinula edodes H 600
F8WPI4
no activity is detected when gentiobiose, lentinan, laminarioligosaccharides, pachyman, CM-pachyman, and lichenan are used as substrates, the purified endo-beta-1,6-glucanase Pus30A catalyzes depolymerization of beta-1,6-glucan endolytically and is highly specific toward beta-1,6-glucan polysaccharide. Pus30A shows hydrolyzing activity against the cell wall components of Lentinula edodes fruiting bodies (54.5% activity compared to pustulan)
-
-
-
additional information
?
-
Hypocrea lixii CECT 2413
-
BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview, substrate specificity, BGN16.3 shows endohydrolytic activity against substrates with beta-1,6-glycosidic linkages, no activity with colloidal chitin, pachyman, starch, cellulose, nigeran, and dextran, no activity with the beta-1,6-disaccharide gentiobiose
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
Hypocrea lixii, Hypocrea lixii CECT 2413
-
BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview
-
-
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-Hydroxy-5-nitrobenzyl bromide
-
-
Dicyclohexylcarbodiimide
-
-
Fe2+
-
-
-
Fe3+
-
-
-
glucono-delta-lactone
-
-
N-Acetylimidazole
-
-
N-bromosuccinimide
-
-
N-ethyl-5-phenylisoxazolium 3'-sulfonate
-
-
p-hydroxymercuribenzoate
-
-
additional information
-
ethanol extracts of various marine invertebrates: Geodinella spherastrosa, Spirastrella digitata, Spirastrella vagabunda, Chondrilla australiensis, Phakelia cardius, Desmoxya lunata, Mycale monanchorata, Clathria frondifera, Clathria lendenfeldi, Iotrochota baculifera, Phorbas sp., Callyspongia diffusa, Callyspongia sp., Callyspongia subarmigera, Arenosclera sp., Amphimedon sp., Oceanapia imperfecta, Sigmadocia symbiotica, Dysidera herbacea, Dysidea fragilis, Thorecta cacos, Ircinia sp.
-
additional information
-
no inhibitory: D-glucono-1,5-lactone at 1-20 mM
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
BGN16.3 is induced rapidly and strongly by fungal cell walls
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.014
-
Gentiotetraose
-
-
0.0753
-
pustulan
-
assumed molecular weight for pustulan 17000 Da, or KM of 1.28 mg/ml
-
0.00012
-
lutean
-
assumed molecular weight for lutean 650000 Da
-
additional information
-
additional information
-
pustulan 0.8 mg/ml
-
additional information
-
additional information
-
pustulan 0.38 mg/ml
-
additional information
-
additional information
-
pustulan 2.78 mg/ml
-
additional information
-
additional information
-
islandican 0.173%
-
additional information
-
additional information
-
pustulan 0.29 mg/ml
-
additional information
-
additional information
-
pustulan 0.4-1.3 mg/ml
-
additional information
-
additional information
-
lutean 1.38 mg/ml, laminarin 1.67 mg/ml
-
additional information
-
additional information
-
pustulan 0.8 mg/ml
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.22
-
Flavobacterium M64
-
-
0.48
-
Neotyphodium sp.
Q8NJN4
-
1.654
-
-
-
5
-
-
intracellular enzyme
5.18
-
-
-
75.4
-
-
extracellular enzyme
83.3
-
-
pH 5.0, 30C
188
-
-
purified enzyme
additional information
-
-
224.7 U/mg, one U being 1 mg of liberated reducing sugar from pustulan after 2 h incubation
additional information
-
-
34.3 reducing sugars corresponding to 4.43 micro mol of glucose/min/mg
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.2
4.6
-
-
4.8
-
-
assay at
5
-
P82288
at 50C
6
-
-
in Tris/succinate buffer
6.5
-
-
in sodium phosphate buffer
6.5
-
P82288
at 40C
additional information
-
-
pH 4 +/- 0.4 for six species
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
5
F8WPI4
-
4
7
-
20% of maximal activity at pH 4.0 and pH 7.0
4.5
6.5
-
at least 80% of maximum activity in this range
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
-
P82288
at pH 6.5
45
-
-
assay at
50
60
F8WPI4
-
50
-
P82288
at pH 6.5
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.1
-
-
acidic chromatofocusing
4.5
-
P82288
isoelectric focusing
4.5
-
-
isoelectric focusing
5.5
-
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Trichoderma sp.
-
-
Manually annotated by BRENDA team
Bacillus circulans WL-12
-
;
-
Manually annotated by BRENDA team
Hypocrea lixii CECT 2313, Neurospora crassa IFO-6068, Penicillium brefeldianum QM 1873, Rhizopus microsporus var. chinensis R-69, Saccharomycopsis fibuligera NCYC 451
-
-
-
Manually annotated by BRENDA team
Lentinula edodes H 600
-
-
-
Manually annotated by BRENDA team
additional information
-
expression profile of BGN16.3, regulation of BGN16.3 expression
Manually annotated by BRENDA team
additional information
Hypocrea lixii CECT 2413
-
expression profile of BGN16.3, regulation of BGN16.3 expression
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Neotyphodium sp.
Q8NJN4
-
Manually annotated by BRENDA team
Trichoderma sp.
-
-
-
Manually annotated by BRENDA team
Neotyphodium sp.
Q8NJN4
-
-
Manually annotated by BRENDA team
-
secretion of the beta-1,6-glucanase multienzyme complex, BGN16.3 is part of the complex
-
Manually annotated by BRENDA team
-
the enzyme is secreted
-
Manually annotated by BRENDA team
Bacillus circulans WL-12
-
;
-
-
Manually annotated by BRENDA team
Hypocrea lixii CECT 2313
-
-
-
-
Manually annotated by BRENDA team
Hypocrea lixii CECT 2413
-
secretion of the beta-1,6-glucanase multienzyme complex, BGN16.3 is part of the complex
-
-
Manually annotated by BRENDA team
Neotyphodium sp. FCB-2002, Neurospora crassa IFO-6068, Penicillium brefeldianum QM 1873, Rhizopus microsporus var. chinensis R-69, Saccharomycopsis fibuligera NCYC 451
-
-
-
-
Manually annotated by BRENDA team
Saccharomycopsis fibuligera NCYC 451
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20000
25000
-
gel filtration
20000
-
-
gel filtration
25000
30000
-
gel filtration
30000
-
-
gel filtration
30500
-
-
sedimentation equilibrium
37000
-
-
gel filtration
43000
-
-
extracellular enzyme, gel filtration
44300
-
-
gel filtration
50000
-
-
gel filtration
51000
-
-
intracellular enzyme, gel filtration
54000
-
-
SDS-PAGE, gel filtration
55000
-
-
PAGE
65000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 44000, SDS-PAGE
?
-
x * 42700, SDS-PAGE
?
-
x * 51000, SDS-PAGE
?
-
x * 43000, SDS-PAGE
?
-
x * 60000, SDS-PAGE
?
P82288
x * 41200, SDS-PAGE
?
Neotyphodium sp.
Q8NJN4
x * 47000, SDS-PAGE
?
F8WPI4
x * 49000, SDS-PAGE
?
Hypocrea lixii CECT 2313
-
x * 51000, SDS-PAGE
-
?
Lentinula edodes H 600
-
x * 49000, SDS-PAGE
-
?
-
x * 47000, SDS-PAGE
-
?
Penicillium brefeldianum QM 1873
-
x * 44000, SDS-PAGE
-
monomer
-
1 * 65000, SDS-PAGE
monomer or dimer
-
? * 46000, BGN16.3, SDS-PAGE
monomer or dimer
Hypocrea lixii CECT 2413
-
? * 46000, BGN16.3, SDS-PAGE
-
additional information
-
N-terminal amino acid sequence
additional information
-
BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex
additional information
Hypocrea lixii CECT 2413
-
BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
no glycoprotein
P82288
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
8
F8WPI4
the enzyme is stable over a broad pH range from pH 4.0 to 8.0 when treated at 4C for 20 h
4.9
5.5
-
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
stable for a long time at 30C, activity decrease at 40C
45
-
-
unstable above, presence of pustulan stabilizes
50
-
-
stable blow 50C
50
-
-
short-term stability up to 50C
50
-
-
stable up to
50
-
P82288
30 min, stable
60
-
F8WPI4
the enzyme is completely inactivated after incubation at 60C for 30 min
70
-
-
inactivated in less than 10 min at 70C
75
-
P82288
30 min, about 20% residual activity
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, 4 months, no loss of activity, -70C, 12 months, no loss of activity
-
-25C, 1 month, less than 10% loss of activity
-
2C, 1 week, 20% loss of activity
-
4C, 6 months, no loss of activity
-
-20C, pH6, 2 months, no loss of activity
-
-20C, repeated freezing and thwaing severely damages enzyme activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
FLAG-tagged Neg1 protein purified by batch adsorption
-
486.5fold
-
140fold
Flavobacterium M64
-
154fold, 68fold expressed in Pichia pastoris
-
native extracellular BGN16.3 26fold to homogeneity by beta-1,6-glycan affinity chromatography, chromatofocusing, and gel filtration
-
ammonium sulfate precipitation, Sepharose Q column chromatography, Superdex 75 gel filtration, and DEAE-Toyopearly column chromatography
F8WPI4
partial
Neotyphodium sp.
Q8NJN4
intracellular enzyme 100fold, extracellular enzyme 75fold
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
into expression/secretion vector pINA1296+FLAG, expressed in Yarrowia lipolytica strain Po1g: MatA, leu2-270, ura3-302::pBR322-URA3, xpr2-322, axp-2, which contains an integrated pBR322 plasmid as a docking platform
-
expression in Pichia pastoris
-
expression in Saccharomyces cerevisiae
-
expression profile of BGN16.3
-
functional expression from yeast expression vector pYESH2 in Saccharomyces cerevisiae strain INVSc1. The recombinant enzyme does not show antifungal activity in yeast
-
-
Neotyphodium sp.
Q8NJN4
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the transcript level of endo-beta-1,6-glucanase Pus30A gene (lepus30a) is significantly increased in fruiting bodies 4 days after harvesting (approximately 18times in gills, 27times in stipes, and 32times in pilei) compared to fresh fruiting bodies
F8WPI4
the transcript level of endo-beta-1,6-glucanase Pus30A gene (lepus30a) is significantly increased in fruiting bodies 4 days after harvesting (approximately 18times in gills, 27times in stipes, and 32times in pilei) compared to fresh fruiting bodies
Lentinula edodes H 600
-
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
Q7M4T0
the 1,6-beta-D-glucanase gene (Neg1) is disrupted by repeat-induced point mutations. 9 nucleotides within the coding region of the gene change from GC to AT. No apparent phenotypic changes are observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide, which affect fungal cell walls or membranes, markedly inhibit the hyphal growth of the mutant at a concentration that does not inhibi growth of the wild-type
additional information
Q7ZA48, -
gene disruption mutant, shows reduced virulence against Agaricus bisporus and diminished ability to use chitin as a carbon source
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
biotechnology
-
conditions for synthesis of enzyme in batch culture
biotechnology
-
maximal overproduction of enzyme is achieved in buffered medium where pH-induced aspartyl proteases are absent or when nitrogen sources such as yeast extract are substrate for these proteases
biotechnology
-
use of enzyme in analysis of yeast cell wall protein