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Literature summary for 3.2.1.75 extracted from
- A combined chemical and enzymatic method to determine quantitatively the polysaccharide components in the cell wall of yeasts (2014), FEMS Yeast Res., 14, 933-947.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the enzyme is used for analysis of yeast polysaccharides cell wall composition in a method combines acid and enzymatic hydrolysis, and involves a chitinase from Streptomyces griseus, an endo/exo-beta-(1,3)-glucanase from Trichoderma species, recombinant endo-beta-(1,6)-glucanase from Trichoderma harzianum, and a beta-glucosidase from Aspergillus niger, method development and evaluation, overview | Trichoderma harzianum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression in Pichia pastoris | Trichoderma harzianum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pustulan + H2O | Trichoderma harzianum | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma harzianum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-(1,6)-glucan + H2O | highly branched beta-(1,6)-glucan, that contains about 75-80% of linear beta-(1,6)-glucose linked units, preared from yeast cell walls | Trichoderma harzianum | ? | - |
? | |
| additional information | no activity with laminarin, chitin, and mannan | Trichoderma harzianum | ? | - |
? | |
| pustulan + H2O | - |
Trichoderma harzianum | ? | - |
? | |
| pustulan + H2O | from Umbilicaria pustulata | Trichoderma harzianum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-(1,6) glucanase | - |
Trichoderma harzianum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Trichoderma harzianum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Trichoderma harzianum |
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Release 2023.1 (January 2023)
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