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Literature summary for 3.2.1.75 extracted from
- An endo-beta-1,6-glucanase involved in Lentinula edodes fruiting body autolysis (2011), Appl. Microbiol. Biotechnol., 91, 1365-1373.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
x * 49000, SDS-PAGE | Lentinula edodes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lentinula edodes | F8WPI4 | - |
- |
| Lentinula edodes H 600 | F8WPI4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Sepharose Q column chromatography, Superdex 75 gel filtration, and DEAE-Toyopearly column chromatography | Lentinula edodes |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit body | - |
Lentinula edodes | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminarin + H2O | 3% activity compared to pustulan | Lentinula edodes | D-glucose + ? | the main product is D-glucose | ? |  |
| laminarin + H2O | 3% activity compared to pustulan | Lentinula edodes H 600 | D-glucose + ? | the main product is D-glucose | ? | |
| additional information | no activity is detected when gentiobiose, lentinan, laminarioligosaccharides, pachyman, CM-pachyman, and lichenan are used as substrates | Lentinula edodes | ? | - |
? | |
| additional information | the purified endo-beta-1,6-glucanase Pus30A catalyzes depolymerization of beta-1,6-glucan endolytically and is highly specific toward beta-1,6-glucan polysaccharide. Pus30A shows hydrolyzing activity against the cell wall components of Lentinula edodes fruiting bodies (54.5% activity compared to pustulan) | Lentinula edodes | ? | - |
? | |
| additional information | no activity is detected when gentiobiose, lentinan, laminarioligosaccharides, pachyman, CM-pachyman, and lichenan are used as substrates | Lentinula edodes H 600 | ? | - |
? | |
| additional information | the purified endo-beta-1,6-glucanase Pus30A catalyzes depolymerization of beta-1,6-glucan endolytically and is highly specific toward beta-1,6-glucan polysaccharide. Pus30A shows hydrolyzing activity against the cell wall components of Lentinula edodes fruiting bodies (54.5% activity compared to pustulan) | Lentinula edodes H 600 | ? | - |
? | |
| pustulan + H2O | high substrate specificity, 100% activity | Lentinula edodes | ? | - |
? | |
| pustulan + H2O | high substrate specificity, 100% activity | Lentinula edodes H 600 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49000, SDS-PAGE | Lentinula edodes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-1,6-glucanase | - |
Lentinula edodes |
| Pus30A | - |
Lentinula edodes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Lentinula edodes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
the enzyme is completely inactivated after incubation at 60°C for 30 min | Lentinula edodes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | - |
Lentinula edodes |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | the enzyme is stable over a broad pH range from pH 4.0 to 8.0 when treated at 4°C for 20 h | Lentinula edodes |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Lentinula edodes | the transcript level of endo-beta-1,6-glucanase Pus30A gene (lepus30a) is significantly increased in fruiting bodies 4 days after harvesting (approximately 18times in gills, 27times in stipes, and 32times in pilei) compared to fresh fruiting bodies | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | endo-beta-1,6-glucanase Pus30A is responsible for the degradation of the cell wall components during fruiting body autolysis after harvest | Lentinula edodes |
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Release 2023.1 (January 2023)
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