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Literature summary for 3.2.1.75 extracted from
- BGN16.3, a novel acidic beta-1,6-glucanase from mycoparasitic fungus Trichoderma harzianum CECT 2413 (2005), FEBS J., 272, 3441-3448.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | BGN16.3 is induced rapidly and strongly by fungal cell walls | Trichoderma harzianum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression profile of BGN16.3 | Trichoderma harzianum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secretion of the beta-1,6-glucanase multienzyme complex, BGN16.3 is part of the complex | Trichoderma harzianum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | 30000 | gel filtration | Trichoderma harzianum |
| 46000 | - |
? * 46000, BGN16.3, SDS-PAGE | Trichoderma harzianum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Trichoderma harzianum | BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview | ? | - |
? |  |
| additional information | Trichoderma harzianum CECT 2413 | BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma harzianum | - |
3 isozymes BGN16.1, BGN16.2, and BGN16.3 | - |
| Trichoderma harzianum CECT 2413 | - |
3 isozymes BGN16.1, BGN16.2, and BGN16.3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular BGN16.3 26fold to homogeneity by beta-1,6-glycan affinity chromatography, chromatofocusing, and gel filtration | Trichoderma harzianum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | expression profile of BGN16.3, regulation of BGN16.3 expression | Trichoderma harzianum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 188 | - |
purified enzyme | Trichoderma harzianum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glucan + H2O | substrate from yeast, 18% of maximal activity | Trichoderma harzianum | ? | - |
? | |
| glucan + H2O | substrate from yeast, 18% of maximal activity | Trichoderma harzianum CECT 2413 | ? | - |
? | |
| laminarin + H2O | 8% of maximal activity | Trichoderma harzianum | ? | - |
? | |
| laminarin + H2O | 8% of maximal activity | Trichoderma harzianum CECT 2413 | ? | - |
? | |
| additional information | BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview | Trichoderma harzianum | ? | - |
? | |
| additional information | substrate specificity, BGN16.3 shows endohydrolytic activity against substrates with beta-1,6-glycosidic linkages, no activity with colloidal chitin, pachyman, starch, cellulose, nigeran, and dextran, no activity with the beta-1,6-disaccharide gentiobiose | Trichoderma harzianum | ? | - |
? | |
| additional information | BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview | Trichoderma harzianum CECT 2413 | ? | - |
? | |
| additional information | substrate specificity, BGN16.3 shows endohydrolytic activity against substrates with beta-1,6-glycosidic linkages, no activity with colloidal chitin, pachyman, starch, cellulose, nigeran, and dextran, no activity with the beta-1,6-disaccharide gentiobiose | Trichoderma harzianum CECT 2413 | ? | - |
? | |
| pustulan + H2O | best substrate | Trichoderma harzianum | oligomers of pustulan | product analysis, most abundant products are di-, tri-, and tetra-beta-1,6-glucosides | ? | |
| pustulan + H2O | best substrate | Trichoderma harzianum CECT 2413 | oligomers of pustulan | product analysis, most abundant products are di-, tri-, and tetra-beta-1,6-glucosides | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer or dimer | ? * 46000, BGN16.3, SDS-PAGE | Trichoderma harzianum |
| More | BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex | Trichoderma harzianum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acidic beta-1,6-glucanase | - |
Trichoderma harzianum |
| BGN16.3 | - |
Trichoderma harzianum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Trichoderma harzianum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Trichoderma harzianum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | 20% of maximal activity at pH 4.0 and pH 7.0 | Trichoderma harzianum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Trichoderma harzianum | acidic chromatofocusing | - |
4.1 |
| Trichoderma harzianum | isoelectric focusing | - |
4.5 |
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Release 2023.1 (January 2023)
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