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Literature summary for 3.2.1.75 extracted from
- Mode of action of a beta-(1->6)-glucanase from Penicillium multicolor (2013), Carbohydr. Res., 366, 6-16.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Penicillium multicolor | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49909 | - |
x * 51000, SDS-PAGE, x * 49909, mass spectrometry | Penicillium multicolor |
| 51000 | - |
x * 51000, SDS-PAGE, x * 49909, mass spectrometry | Penicillium multicolor |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gentiooctaose + H2O | Penicillium multicolor | - |
? | - |
? |  |
| gentiooctaose + H2O | Penicillium multicolor LAM7153 | - |
? | - |
? | |
| pustulan + H2O | Penicillium multicolor | - |
? | - |
? | |
| pustulan + H2O | Penicillium multicolor LAM7153 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium multicolor | - |
- |
- |
| Penicillium multicolor LAM7153 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 244.3fold from culture supernatant by ammonium sulfate fractionation, cation exchange and affinity chromatography using gentiotetraose as ligand | Penicillium multicolor |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| gentiononaose + 2 H2O = gentiobiose + gentiotriose + gentiotetraose | hydrolytic cooperation of both an endo-type and saccharifying-type reaction by a single enzyme, which plays a bifunctional role, leading to complete hydrolysis of the glucan. the enzyme varies its mode of action depending on the chain length derived from the glucan | Penicillium multicolor |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 164.1 | - |
purified native enzyme, pH 4.0, 50°C | Penicillium multicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-gentiohexaoside + H2O | - |
Penicillium multicolor | ? | - |
? | |
| 4-nitrophenyl beta-gentiohexaoside + H2O | - |
Penicillium multicolor LAM7153 | ? | - |
? | |
| 4-nitrophenyl beta-gentiopentaoside + H2O | - |
Penicillium multicolor | ? | - |
? | |
| 4-nitrophenyl beta-gentiotetraoside + H2O | - |
Penicillium multicolor | ? | - |
? | |
| gentiooctaose + H2O | - |
Penicillium multicolor | ? | - |
? | |
| gentiooctaose + H2O | - |
Penicillium multicolor LAM7153 | ? | - |
? | |
| methyl beta-gentiobioside + H2O | low activity | Penicillium multicolor | methyl beta-D-glucoside + beta-D-glucose | - |
? | |
| methyl beta-gentiohexaoside + H2O | the major cleavages occur at bonds 2, 3, and 4 in approximately equal proportion, the nonreducing end glucosyl residues of Gen4-6 beta-OMe are either not or scarcely cleaved by the enzyme | Penicillium multicolor | methyl beta-D-glucoside + 2 beta-D-glucose + gentiobiose | - |
? | |
| methyl beta-gentiopentaoside + H2O | the major cleavages occur at bonds 2, 3, and 4 in approximately equal proportion | Penicillium multicolor | methyl beta-D-glucoside + 3 beta-D-glucose | - |
? | |
| methyl beta-gentiotetraoside + H2O | cleavage at the second (bond 2) and third (bond 3) glucosidic bonds from the methyl group | Penicillium multicolor | methyl beta-D-glucoside + 2 beta-D-glucose | - |
? | |
| methyl beta-gentiotrioside + H2O | the second glucosidic bond (bond 2) from the methyl group is cut preferentially | Penicillium multicolor | methyl beta-D-glucoside + gentiobiose | Glc beta-OMe and Gen2 beta-OMe in the ratio 91:9 | ? | |
| additional information | the enzyme is a specific beta-(1->6)-glucanase, it shows no activity with soluble starch, carboxymethyl cellulose, curdlan from Alcaligenes faecalis, and 4-nitrophenyl beta-gentiobioside, it shows poor activity with yeast glucan and 4-nitrophenyl beta-gentiotrioside. The cleavage point of 4-nitrophenyl oligosacchride derivatives with DP 4 or higher shifts such that the enzyme keeps away from the 4-nitrophenyl group at the terminus, in all the tested substrates, release of 4-nitrophenol is not observed. Gentiooligosaccharides (DP 3-9) are prepared from the starting substance gentiobiose by the sequential transglycosylation reactions of beta-glucosidase and beta-(1->6)-glucanase | Penicillium multicolor | ? | - |
? | |
| additional information | the enzyme is a specific beta-(1->6)-glucanase, it shows no activity with soluble starch, carboxymethyl cellulose, curdlan from Alcaligenes faecalis, and 4-nitrophenyl beta-gentiobioside, it shows poor activity with yeast glucan and 4-nitrophenyl beta-gentiotrioside. The cleavage point of 4-nitrophenyl oligosacchride derivatives with DP 4 or higher shifts such that the enzyme keeps away from the 4-nitrophenyl group at the terminus, in all the tested substrates, release of 4-nitrophenol is not observed. Gentiooligosaccharides (DP 3-9) are prepared from the starting substance gentiobiose by the sequential transglycosylation reactions of beta-glucosidase and beta-(1->6)-glucanase | Penicillium multicolor LAM7153 | ? | - |
? | |
| pustulan + H2O | - |
Penicillium multicolor | ? | - |
? | |
| pustulan + H2O | the enzyme completely hydrolyzes pustulan in three steps: in the initial stage, the enzyme quickly cleaves the glucan with a pattern resembling an endo-hydrolase to produce a short-chain glucan (degree of polymerization DP 45) as an intermediate. In the midterm stage, the resulting short-chain glucan is further cleaved into two fractions corresponding to DP 15-7 and DP 2-4 with great regularity. In the final stage, the lower oligomers corresponding to DP 3 and DP 4 are very slowly hydrolyzed into glucose and gentiobiose | Penicillium multicolor | ? | - |
? | |
| pustulan + H2O | - |
Penicillium multicolor LAM7153 | ? | - |
? | |
| pustulan + H2O | the enzyme completely hydrolyzes pustulan in three steps: in the initial stage, the enzyme quickly cleaves the glucan with a pattern resembling an endo-hydrolase to produce a short-chain glucan (degree of polymerization DP 45) as an intermediate. In the midterm stage, the resulting short-chain glucan is further cleaved into two fractions corresponding to DP 15-7 and DP 2-4 with great regularity. In the final stage, the lower oligomers corresponding to DP 3 and DP 4 are very slowly hydrolyzed into glucose and gentiobiose | Penicillium multicolor LAM7153 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 51000, SDS-PAGE, x * 49909, mass spectrometry | Penicillium multicolor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-(1->6)-glucanase | - |
Penicillium multicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Penicillium multicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified native enzyme, inactivation above | Penicillium multicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Penicillium multicolor |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 9 | purified native enzyme, 4°C, 24 h, over 90% activity remaining | Penicillium multicolor |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the hydrolytic cooperation of both an endo-type and saccharifying-type reaction by a single enzyme, which plays a bifunctional role, leads to complete hydrolysis of the glucan. Thus, beta-(1->6)-glucanase varies its mode of action depending on the chain length derived from the glucan | Penicillium multicolor |
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