3.2.1.68: isoamylase
This is an abbreviated version!
For detailed information about isoamylase, go to the full flat file.
Word Map on EC 3.2.1.68
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3.2.1.68
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salivary
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lipase
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amylopectin
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hyperamylasemia
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isoenzymes
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alpha-amylase
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pancreas
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phosphorylase
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amylose
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pullulanase
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exocrine
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abdominal
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endosperm
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dextrin
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glucans
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phytoglycogen
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salivary-type
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nonpancreatic
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amylolytic
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pseudocysts
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s-type
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beta-amylase
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dextrinase
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pancreatic-type
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macroamylasemia
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amylo-1,6-glucosidase
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extrapancreatic
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glucanotransferase
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phadebas
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analysis
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4-alpha-glucanotransferase
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nutrition
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maltooligosaccharides
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glycogenolytic
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maltotriose
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alpha-1,4
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isomaltose
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alpha-glucans
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waxy
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hyperenzymemia
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glycogenin
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granule-bound
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medicine
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food industry
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synthesis
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degradation
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detergent
- 3.2.1.68
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salivary
- lipase
- amylopectin
-
hyperamylasemia
- isoenzymes
- alpha-amylase
- pancreas
- phosphorylase
- amylose
- pullulanase
-
exocrine
- abdominal
- endosperm
- dextrin
- glucans
- phytoglycogen
-
salivary-type
-
nonpancreatic
-
amylolytic
- pseudocysts
-
s-type
- beta-amylase
-
dextrinase
-
pancreatic-type
-
macroamylasemia
- amylo-1,6-glucosidase
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extrapancreatic
-
glucanotransferase
-
phadebas
- analysis
- 4-alpha-glucanotransferase
- nutrition
- maltooligosaccharides
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glycogenolytic
- maltotriose
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alpha-1,4
- isomaltose
- alpha-glucans
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waxy
-
hyperenzymemia
- glycogenin
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granule-bound
- medicine
- food industry
- synthesis
- degradation
- detergent
Reaction
Synonyms
alkaline isoamylase, alpha 1,6-glucan hydrolase, alpha-1,6-glucosidase, ArDBEp, AtISA1, AtISA1/AtISA2 isoamylase, AtISA2, AtISA3, bacterial isoamylase, CrISA1, Cythophaga isoamylase, DBE, DBEI, debranching enzyme, GlgX, glycogen 6-glucanohydrolase, glycogen alpha-1,6-glucanohydrolase, glycogen debranching enzyme, glycogen-6-glucanogydrolase, glycogen-6-glucanohydrolase, glycogen-debranching enzyme, IAM, ISA, ISA1, ISA1-type DBE, ISA2, ISA3, isoamylase, isoamylase 1, isoamylase 2, isoamylase 3, isoamylase II, isoamylase-type debranching enzyme, isoamylase-type starch debranching enzyme 1, isoamylase-type starch debranching enzyme 2, isoamylase-type starch-debranching enzyme, isoamylase1, maize Sugary-1 isoamylase, OsISA1, OsISA2, pancreatic isoamylase isoA, plant isoamylase, potato isoamylase, Pseudomonas isoamylase, PvISA1, PvISA2, PvISA3, rice isoamylase, starch debranching enzyme, SU1 isoamylase, TreX, yeast isoamylase, ZmISA1, ZmISA2
ECTree
3.2.1.68 isoamylaseAdvanced search results
results (
results (Engineering
Engineering on EC 3.2.1.68 - isoamylase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D367A
site-directed mutagenesis of the catalytic nucleophile Asp-367 in ISA1, the mutation abolishes the catalytic activity of the enzyme complex
G608A
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the kcat/Km values and the specific activity of the mutant are decreased as compared to the wild type enzyme
G608K
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the kcat/Km values and the specific activity of the mutant are decreased as compared to the wild type enzyme
G608V
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the kcat/Km values of the mutant are promoted by 49% and the specific activity increases by 33% as compared to the wild type enzyme
N513A
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the kcat/Km values of the mutant are slightly decreased and the specific activity is decreased as compared to the wild type enzyme
N513K
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the kcat/Km values and the specific activity of the mutant are decreased as compared to the wild type enzyme
N513V
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the kcat/Km values and the specific activity of the mutant are strongly decreased as compared to the wild type enzyme
R505A
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the kcat/Km values of the mutant are promoted and the specific activity is decreased as compared to the wild type enzyme
R505E
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the acidic stability is enhanced and the specific activity of the mutant is increased by 13% compared to the wild type enzyme
R505P
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the thermal stability of the mutant is enhanced compared to the wild type enzyme
G608A
Lederbergia lentus JNU3
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the kcat/Km values and the specific activity of the mutant are decreased as compared to the wild type enzyme
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G608V
Lederbergia lentus JNU3
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the kcat/Km values of the mutant are promoted by 49% and the specific activity increases by 33% as compared to the wild type enzyme
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R505A
Lederbergia lentus JNU3
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the kcat/Km values of the mutant are promoted and the specific activity is decreased as compared to the wild type enzyme
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R505E
Lederbergia lentus JNU3
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the acidic stability is enhanced and the specific activity of the mutant is increased by 13% compared to the wild type enzyme
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R505P
Lederbergia lentus JNU3
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the thermal stability of the mutant is enhanced compared to the wild type enzyme
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additional information
additional information
construction of a single mutant line isa2-2 by T-DNA insertion mutagenesis. The isa1-1 isa2-2 double mutant line is obtained by a cross between the two corresponding single mutants and screening of the progeny by PCR amplification of genomic DNA using a T-DNA end primer and gene-specific primers. The phenotype of the isa1-1 isa2-2 double mutant is complemented but the ZmISA1 activity
additional information
construction of a single mutant line isa2-2 by T-DNA insertion mutagenesis. The isa1-1 isa2-2 double mutant line is obtained by a cross between the two corresponding single mutants and screening of the progeny by PCR amplification of genomic DNA using a T-DNA end primer and gene-specific primers. The phenotype of the isa1-1 isa2-2 double mutant is complemented but the ZmISA1 activity
additional information
construction of single mutant line isa1-1 by T-DNA insertion mutagenesis. The isa1-1 isa2-2 double mutant line is obtained by a cross between the two corresponding single mutants and screening of the progeny by PCR amplification of genomic DNA using a T-DNA end primer and gene-specific primers. The phenotype of the isa1-1 isa2-2 double mutant is complemented but the ZmISA1 activity, Zea mays ISA1 subunit is expressed via transfection with Agrobacterium tumefaciens strain GV3101 in Arabidopsis thaliana lacking endogenous ISA1 or lacking both native ISA1 and ISA2. The maize protein functions in Arabidopsis leaves to support nearly normal starch metabolism in the absence of any native ISA1 or ISA2
additional information
construction of single mutant line isa1-1 by T-DNA insertion mutagenesis. The isa1-1 isa2-2 double mutant line is obtained by a cross between the two corresponding single mutants and screening of the progeny by PCR amplification of genomic DNA using a T-DNA end primer and gene-specific primers. The phenotype of the isa1-1 isa2-2 double mutant is complemented but the ZmISA1 activity, Zea mays ISA1 subunit is expressed via transfection with Agrobacterium tumefaciens strain GV3101 in Arabidopsis thaliana lacking endogenous ISA1 or lacking both native ISA1 and ISA2. The maize protein functions in Arabidopsis leaves to support nearly normal starch metabolism in the absence of any native ISA1 or ISA2
additional information
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construction of single mutant line isa1-1 by T-DNA insertion mutagenesis. The isa1-1 isa2-2 double mutant line is obtained by a cross between the two corresponding single mutants and screening of the progeny by PCR amplification of genomic DNA using a T-DNA end primer and gene-specific primers. The phenotype of the isa1-1 isa2-2 double mutant is complemented but the ZmISA1 activity, Zea mays ISA1 subunit is expressed via transfection with Agrobacterium tumefaciens strain GV3101 in Arabidopsis thaliana lacking endogenous ISA1 or lacking both native ISA1 and ISA2. The maize protein functions in Arabidopsis leaves to support nearly normal starch metabolism in the absence of any native ISA1 or ISA2
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additional information
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construction of a single mutant line isa2-2 by T-DNA insertion mutagenesis. The isa1-1 isa2-2 double mutant line is obtained by a cross between the two corresponding single mutants and screening of the progeny by PCR amplification of genomic DNA using a T-DNA end primer and gene-specific primers. The phenotype of the isa1-1 isa2-2 double mutant is complemented but the ZmISA1 activity
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additional information
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mutant sta8, about 35% residual enzymic activity, loss of two out of three activity bands in zymogram gels, loss of about 50% of mass of the multimeric complex
additional information
functional complementation of sta8 mutant strains BafV13 and BafO6 with ISA2 or ISA2-HA, overview
additional information
functional complementation of sta8 mutant strains BafV13 and BafO6 with ISA2 or ISA2-HA, overview
additional information
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functional complementation of sta8 mutant strains BafV13 and BafO6 with ISA2 or ISA2-HA, overview
additional information
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functional complementation of sta8 mutant strains BafV13 and BafO6 with ISA2 or ISA2-HA, overview
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additional information
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Cassava starch is debranched by treatment with isoamylase and pullulanase and the yield of resistant starch type III, method optimization with respect to starch solids concentration, incubation time, and enzyme concentration using central composite rotatable design
additional information
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mutations in the N-terminal region result in a sharp increase in alpha-1,4-transferase activity and a reduced level of alpha-1,6-glucosidase activity, overview
additional information
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transgenic plants expressing antisense RNA for Stisa1 or Stisa2, accumulate small amounts of soluble glucan and large numbers of tiny starch granules
