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3.2.1.68: isoamylase

This is an abbreviated version!
For detailed information about isoamylase, go to the full flat file.

Word Map on EC 3.2.1.68

Reaction

alpha-Glcp-(1-3)-alpha-Glcp-(1-6)-[alpha-Glcp-(1-3)-alpha-Glcp-(1-6)-alpha-Glcp-(1-3)-alpha-Glcp-(1-3)]-alpha-Glcp-(1-3)-alpha-Glcp
+ 2 H2O = 2 alpha-Glcp-(1-3)-alpha-Glcp +
alpha-Glcp-(1-3)-alpha-Glcp-(1-3)-alpha-Glcp-(1-3)-alpha-Glcp

Synonyms

alkaline isoamylase, alpha 1,6-glucan hydrolase, alpha-1,6-glucosidase, ArDBEp, AtISA1, AtISA1/AtISA2 isoamylase, AtISA2, AtISA3, bacterial isoamylase, CrISA1, Cythophaga isoamylase, DBE, DBEI, debranching enzyme, GlgX, glycogen 6-glucanohydrolase, glycogen alpha-1,6-glucanohydrolase, glycogen debranching enzyme, glycogen-6-glucanogydrolase, glycogen-6-glucanohydrolase, glycogen-debranching enzyme, IAM, ISA, ISA1, ISA1-type DBE, ISA2, ISA3, isoamylase, isoamylase 1, isoamylase 2, isoamylase 3, isoamylase II, isoamylase-type debranching enzyme, isoamylase-type starch debranching enzyme 1, isoamylase-type starch debranching enzyme 2, isoamylase-type starch-debranching enzyme, isoamylase1, maize Sugary-1 isoamylase, OsISA1, OsISA2, pancreatic isoamylase isoA, plant isoamylase, potato isoamylase, Pseudomonas isoamylase, PvISA1, PvISA2, PvISA3, rice isoamylase, starch debranching enzyme, SU1 isoamylase, TreX, yeast isoamylase, ZmISA1, ZmISA2

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.68 isoamylase

Purification

Purification on EC 3.2.1.68 - isoamylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE cellulose column chromatography, CM-cellulose column chromatography, and DEAE-Sephadex gel filtration
-
ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration
-
copurification of native isozymes ISA1 and ISA2 partially from leaves by gel filtration, copurification of TAP-tagged ISA1 and ISA2 from Escherichia coli strain BL21(DE3) by tandem-affinity chromatography on TEV and calmodulin, followed by gel filtration
HiTrap chelating column chromatography
-
HiTrapQ column chromatography and Superdex 200 gel filtration
HitrapQ column chromatography and TSKgel DEAE-5PW column chromatography
HitrapQ column chromatography and TSKgel DEAE-5PW gel filtration
-
native extracellular enzyme 369fold from culture supernatant by ultrafiltration, ammonium sulfate fractionation, gel filtration, two different steps of anion exchange chromatography, and dialysis, to homogeneity
native subunit ISA2 from leaves by anion exchange chromatography, ultrafiltration, and gel filtration, separation from subunit ISA1
B6U0X5
native subunit ISAI from leaves by anion exchange chromatography, ultrafiltration, and gel filtration, separation from subunit ISA2
B6U0X5
Q sepharose column chromatography
raw starch desorption adsorption
recombinant C-terminally His8-tagged single subunit ISA1 or ISA1 in complex with His8-tagged ISA2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant C-terminally His8-tagged single subunit ISA2 or His8-tagged ISA2 in complex with ISA1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged subunit ISA1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant protein by His tag column chromatography
-
recombinant wild-type and His-tagged enzyme
-
recombinant wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography
-
wild type enzyme by HitrapQ HP and TSKgel G3000SWXL column chromatography, recombinant OsISA2 by HitrapQ HP column chromatography
O80403