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3.2.1.68: isoamylase

This is an abbreviated version!
For detailed information about isoamylase, go to the full flat file.

Word Map on EC 3.2.1.68

Reaction

alpha-Glcp-(1-3)-alpha-Glcp-(1-6)-[alpha-Glcp-(1-3)-alpha-Glcp-(1-6)-alpha-Glcp-(1-3)-alpha-Glcp-(1-3)]-alpha-Glcp-(1-3)-alpha-Glcp
+ 2 H2O = 2 alpha-Glcp-(1-3)-alpha-Glcp +
alpha-Glcp-(1-3)-alpha-Glcp-(1-3)-alpha-Glcp-(1-3)-alpha-Glcp

Synonyms

alkaline isoamylase, alpha 1,6-glucan hydrolase, alpha-1,6-glucosidase, ArDBEp, AtISA1, AtISA1/AtISA2 isoamylase, AtISA2, AtISA3, bacterial isoamylase, CrISA1, Cythophaga isoamylase, DBE, DBEI, debranching enzyme, GlgX, glycogen 6-glucanohydrolase, glycogen alpha-1,6-glucanohydrolase, glycogen debranching enzyme, glycogen-6-glucanogydrolase, glycogen-6-glucanohydrolase, glycogen-debranching enzyme, IAM, ISA, ISA1, ISA1-type DBE, ISA2, ISA3, isoamylase, isoamylase 1, isoamylase 2, isoamylase 3, isoamylase II, isoamylase-type debranching enzyme, isoamylase-type starch debranching enzyme 1, isoamylase-type starch debranching enzyme 2, isoamylase-type starch-debranching enzyme, isoamylase1, maize Sugary-1 isoamylase, OsISA1, OsISA2, pancreatic isoamylase isoA, plant isoamylase, potato isoamylase, Pseudomonas isoamylase, PvISA1, PvISA2, PvISA3, rice isoamylase, starch debranching enzyme, SU1 isoamylase, TreX, yeast isoamylase, ZmISA1, ZmISA2

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.68 isoamylase

Molecular Weight

Molecular Weight on EC 3.2.1.68 - isoamylase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
gel filtration
101155
1 * 100000, SDS-PAGE, 1 * 101155, sequence calculation
120000
-
-
141000
-
isoamylase II, gel filtration
150000
x * 150000, ISA1 and ISA2 as stable dimer, SDS-PAGE
158000
B6U0X5
ISA2 dimer, gel filtration and analytical ultracentrifugation
178000
-
native enzyme
180000
subunit ISA1, crystal structure analysis and SDS-PAGE
300000
wild type endosperm contains three high molecular mass ISA complexes resolved by gel filtration and native-PAGE. Two complexes of approximately 400 kDa contain both isoform ISA1 and isoform ISA2, and an approximately 300 kDa complex contains isoform ISA1 but not isoform ISA2
340000
-
native enzyme, gel filtration, TSKgel G3000SWxl
370000
gel permeation chromatography
400000
wild type endosperm contains three high molecular mass ISA complexes resolved by gel filtration and native-PAGE. Two complexes of approximately 400 kDa contain both isoform ISA1 and isoform ISA2, and an approximately 300 kDa complex contains isoform ISA1 but not isoform ISA2
420000
O80403
SDS-PAGE, homooligomer, five OsISA1
450000
native ISA1-ISA2 complex, gel filtration
46000
-
2 * 46000, sedimentation equilibrium
490000
-
native enzyme, gel filtration, TSKgel G4000SWxl
50000
-
2 subunits, not linked covalently
510000
O80403
SDS-PAGE, heterooligomer, hetero-hexamer composed of five OsISA1 and one OsISA2
52000
-
2 * 52000, gel filtration
520000
-
gel filtration
60000
1 * 60000, SDS-PAGE
65000
74000
-
x * 74100, deduced from gene sequence, x * 74000, SDS-PAGE
74100
-
x * 74100, deduced from gene sequence, x * 74000, SDS-PAGE
75000
750000
recombinant ISA1-ISA2 complex, gel filtration
79000
80000
80810
-
SB-15, DNA sequence analysis
83000
830000
85000
86000 - 94000
87000
88000
-
calculated by comparison with standard proteins
90000
-
undegraded polypeptide chain
90500
x * 90500, about, sequence calculation
93000
94000
95000
additional information