3.2.1.68: isoamylase

This is an abbreviated version!
For detailed information about isoamylase, go to the full flat file.

Reaction

Synonyms

alkaline isoamylase, alpha 1,6-glucan hydrolase, alpha-1,6-glucosidase, ArDBEp, AtISA1, AtISA1/AtISA2 isoamylase, AtISA2, AtISA3, bacterial isoamylase, CrISA1, Cythophaga isoamylase, DBE, DBEI, debranching enzyme, GlgX, glycogen 6-glucanohydrolase, glycogen alpha-1,6-glucanohydrolase, glycogen debranching enzyme, glycogen-6-glucanogydrolase, glycogen-6-glucanohydrolase, glycogen-debranching enzyme, IAM, ISA, ISA1, ISA1-type DBE, ISA2, ISA3, isoamylase, isoamylase 1, isoamylase 2, isoamylase 3, isoamylase II, isoamylase-type debranching enzyme, isoamylase-type starch debranching enzyme 1, isoamylase-type starch debranching enzyme 2, isoamylase-type starch-debranching enzyme, isoamylase1, maize Sugary-1 isoamylase, OsISA1, OsISA2, pancreatic isoamylase isoA, plant isoamylase, potato isoamylase, Pseudomonas isoamylase, PvISA1, PvISA2, PvISA3, rice isoamylase, starch debranching enzyme, SU1 isoamylase, TreX, yeast isoamylase, ZmISA1, ZmISA2

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.68 isoamylase

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Results	in table
2	Activating Compound
349	AA Sequence
36	Application
1	CAS Registry Number
41	Cloned(Commentary)
8	Crystallization (Commentary)
1015	Disease/ Diagnostics
4	Expression
38	General Information
3	General Stability
59	Inhibitors
13	KM Value [mM]
19	Localization
5	Metals/Ions
64	Molecular Weight [Da]
43	Natural Substrates/ Products (Substrates)
104	Organism
8	Pathway
5	PDB ID
19	pH Optimum
6	pH Range
6	pH Stability
27	Protein Variants
37	Purification (Commentary)
1	Reaction
7	Reaction Type
85	Reference
31	Source Tissue
34	Specific Activity [micromol/min/mg]
3	Storage Stability
230	Substrates and Products (Substrate)
47	Subunits
150	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
3	Temperature Range [°C]
16	Temperature Stability [°C]
10	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 3.2.1.68 - isoamylase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

10 - 40

Oryza sativa

-

temperature treatment does not influence the enzyme activity

136815

21

Saccharolobus solfataricus

-

recombinant wild-type and His-tagged enzyme are more stable at room temperature than at 4°C

666093

30 - 70

Bacillus sp. (in: Bacteria)

L0C9D3

purified native enzyme, pH 6.0, 1 h, over 70% activity remaining

732278

4

Saccharolobus solfataricus

-

recombinant wild-type and His-tagged enzyme are more stable at room temperature than at 4°C

666093

40

2 entries

40 - 50

Oryza sativa

O80403

hetero-oligomer, loss of 50% activity

682469

45

2 entries

50

Solanum tuberosum

-

amylopectin, incubated in the absence of substrate enzyme stable only up to 45°C

136800

60

Pseudomonas amyloderamosa

-

activity decreased about 95%

136806

65

Pseudomonas amyloderamosa

-

heating 10 min at 65°C results in complete loss of activity

136806

75

Saccharolobus solfataricus

-

2 h, recombinant wild-type enzyme and His-tagged enzyme are sstable

666093

80

3 entries

40

Bacillus sp. (in: Bacteria)

-

stable up to 40°C, at temperature below 30°C and above 65°C enzyme is almost inactive

136809

40

Oryza sativa

O80403

homooligomer, complete loss of activity

682469

45

Pseudomonas amyloderamosa

-

not stable above

136806

45

Solanum tuberosum

-

-

136800

80

Bacillus sp. (in: Bacteria)

L0C9D3

purified native enzyme, pH 6.0, 1 h, inactivation

732278

80

Lederbergia lentus

-

the wild type enzyme loses 50% of its initial activity after 1 h at 80°C

751254

80

Saccharolobus solfataricus

-

2 h, recombinant wild-type enzyme and His-tagged enzyme are sstable

666093