3.2.1.67: galacturonan 1,4-alpha-galacturonidase
This is an abbreviated version!
For detailed information about galacturonan 1,4-alpha-galacturonidase, go to the full flat file.
Word Map on EC 3.2.1.67
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3.2.1.67
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pectin
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tomato
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ripening
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cellulase
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ethylene
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aspergillus
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softening
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methylesterase
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pectate
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polygalacturonic
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firmness
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erwinia
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ripe
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pgips
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postharvest
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niger
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pectinolytic
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esculentum
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carotovora
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pectinesterase
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pectinases
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abscission
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lycopersicon
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botrytis
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3.2.1.15
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xylanase
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galacturonic
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cinerea
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wall-degrading
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oligogalacturonides
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ripening-related
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pectinmethylesterase
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climacteric
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chrysanthemi
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banana
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homogalacturonans
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sclerotinia
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rotten
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food industry
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firmer
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1-methylcyclopropene
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cell-wall-degrading
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rhamnogalacturonan
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fruit-specific
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pollen-specific
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methylesterification
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analysis
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soft-rotting
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endopgs
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expansum
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nutrition
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pectin-degrading
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synthesis
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expansins
- 3.2.1.67
- pectin
- tomato
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ripening
- cellulase
- ethylene
- aspergillus
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softening
-
methylesterase
- pectate
- polygalacturonic
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firmness
- erwinia
-
ripe
-
pgips
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postharvest
- niger
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pectinolytic
- esculentum
- carotovora
- pectinesterase
- pectinases
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abscission
- lycopersicon
- botrytis
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3.2.1.15
- xylanase
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galacturonic
- cinerea
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wall-degrading
- oligogalacturonides
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ripening-related
- pectinmethylesterase
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climacteric
- chrysanthemi
- banana
- homogalacturonans
- sclerotinia
-
rotten
- food industry
-
firmer
- 1-methylcyclopropene
-
cell-wall-degrading
- rhamnogalacturonan
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fruit-specific
-
pollen-specific
-
methylesterification
- analysis
-
soft-rotting
- endopgs
- expansum
- nutrition
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pectin-degrading
- synthesis
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expansins
Reaction
Synonyms
alkaline exo-polygalacturonase, carrot exopolygalacturonase, CbPelA, corn pollen polygalacturonase, EPG, exo polygalacturonase, exo-D-galacturonanase, exo-D-galacturonase, Exo-PG, exo-PGase, exo-PGI, exo-PGII, exo-PGU B, exo-poly alpha galactouronosidase, exo-polygalacturonanase, exo-polygalacturonase, exolytic PGase, exoPG, exoPG1, exoPG2, exoPGase, exopoly-D-galacturonase, exopolygalacturonanase, exopolygalacturonase, exopolygalacturonase B, exopolygalacturonase X, Galacturan 1,4-alpha-galacturonidase, GH28, macerating enzyme, More, MpPG1, MpPG3, oligogalacturonate hydrolase, peach exopolygalacturonase, PECI, pectinase, PelB, PG III, PGase, PGase I, PGC2, PGC3, PGI, poly 1,4-alpha-D-galacturonide-galacturonohydrolase, Poly(1,4-alpha-D-galacturonide)galacturonohydrolase, poly(galacturonate)hydrolase, poly-(1-4)-alpha-D-galactosiduronate glycanohydrolase, polygalacturonanase, polygalacturonase, polymethylgalacturonase, RPG15, RPG16, SSXPG1, SSXPG2, TM0437, TmGalU, YeGH2
ECTree
3.2.1.67 galacturonan 1,4-alpha-galacturonidaseAdvanced search results
results (
results (Temperature Stability
Temperature Stability on EC 3.2.1.67 - galacturonan 1,4-alpha-galacturonidase
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
10 - 40
the enzyme retains more than 70% activity after 24 h incubation at 10-40
10 - 50
100
20 - 60
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at pH 4.8 the activity of the purified PGase I increases from 20 to 60°C, decreasing rapidly beyond
30
30 - 50
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the purified enzyme is stable between 30 and 50°C, retaining more than 90% of its activity after 4 h, but the thermal stability of the enzyme obviously declines at 60-70°C
30 - 60
30 - 70
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about 55% activity at 30-40°C, about 45% activity at 50°C, about 25% activity at 60°C, about 15% activity at 70°C
37 - 60
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the respective calculated isoform exoPG1 stabilities at pH 4.0 after 2 h incubation at 37, 50, and 60°C are 95%, 79%, and 54%. Under acidic conditions (pH 2.2), the residual activity after 2 h incubation at the same temperatures decreases to 48%, 36%, and 16%, respectively
45
5 - 35
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purified enzyme, absence of substrate, 1 h, 85-100% activity remaining
5 - 55
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purified enzyme, absence of substrate, 1 h, 10 mM Ca2+, 80-100% activity remaining
50
50 - 60
50 - 70
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the immobilized polygalacturonase is most stable at 50°C and least stable at 70°C
55
55 - 80
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phase I of isoform exo-PGI shows half-lives of 6.83 and 2.39 min at 55°C and 80°C, respectively, whereas phase II of the enzyme exhibits a half-life of 63.57 and 22.72 min at 55°C and 80°C, respectively
60
60 - 70
60 - 90
70
80
86.3
Thermotoga thermophilus
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the enzyme is hyperthermostable with 50% of activity remaining at 86.3°C after 1 h incubation
93.9
50% of the initial enzyme activity remains after 1 h incubation at 93.9°C
10 - 50
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the enzyme retains its maximum activity between 10 and 40°C for 30 min. The maximum stability is up to 40°C and the activity declines sharply above this temperature. Approximately 53% of the activity is retained at 50°C
10 - 50
recombinant enzyme retains more than 50% activity at 10-50°C in 100 mM potassium phosphate buffer, pH 5.0, after 2 h incubation
100
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the purified enzyme is heat labile as boiling for 5 min reduces approximately 80% of enzyme activity
30 - 60
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thermal stability between 30 and 60°C with more than 70% activity remaining after 24 h
50
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the enzyme maintains residual activity of greater than 80% at 50°C after 15 h of incubation and after 24 h its activity is over 60% of its maximum activity
50 - 60
purified recombinant His-tagged enzyme, pH 6.0, 5-10 min, stable at 50°C, loss of over 80% activity at 60°C
50 - 60
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purified enzyme, completely stable for 1 h at 50°C. At 55°C and 60°C, the activity decreases by 55% and 90%, respectively. After 50 min inactivation at 60°C
55
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30 min barely inactivates enzyme, one-half of activity destroyed when heated to 65°C, completely destroyed at 80°C
55
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30 min barely inactivates enzyme, one-half of activity destroyed when heated to 65°C, completely destroyed at 80°C
60
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30 min, 75% residual activity, exo-PG1, 25% residual activity, exo-PG2
60
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stable below 60°C, rapidly inactivated above 60°C, 10 min at 65°C, 40% activity lost
60
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purified recombinant proteins, pH 5.0, the wild-type full-length enzyme is completely stable up to, the catalytic domain of polygalacturonase loses 30% within 1 h
60
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purified enzyme, loss of 50% activity in absence of Ca2+ after 25 min, in presence of 10 mM Ca2+ after 37 min
60 - 70
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when the crude enzyme is heated at 60°C for 30 min, 80% of the original activity is lost, and at incubation at 70°C for 30 min., the enzyme activity is abolished
60 - 90
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the enzyme retains 100% activity after 5 h at 60°C, more than 80% activity after 5 h at 70°C, about 80% activity after 2 h at 80°C, more than 50% activity after 5 h at 80°C, and about 80% activity after 2 h at 90°C
60 - 90
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at 60°C, the sodium-alginate-matrix-immobilized enzyme retains more than 90% of its activity after 60 min of heating, while free enzyme does not show any activity at the same temperature. At 90°C, the half-life value of covalently immobilized enzyme is recorded 138.6 min while adsorbed enzyme shows a half-life of 115.5 min, whereas, the free enzyme displays 2.39 min half-life at 80°C
70
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purified enzyme, absence of substrate, 1 h, 45% activity remaining, 70% in presence of 10 mM Ca2+
