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Literature summary for 3.2.1.67 extracted from

  • Gomes, E.; Leite, R.S.; da Silva, R.; Silva, D.
    Purification of an exopolygalacturonase from Penicillium viridicatum RFC3 produced in submerged fermentation (2009), Int. J. Microbiol., 2009, 631942.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
Ca2+ enhances the stability of enzyme and its activity by 30% Penicillium viridicatum

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 7% inhibition at 10 mM, 13% inhibition at 2 mM Penicillium viridicatum
Al3+ 33% inhibition at 2 mM, no inhibition at 10 mM Penicillium viridicatum
Ba2+ 10% inhibition at 2-5 mM Penicillium viridicatum
Co2+ 7% inhibition at 10 mM, 7% activation at 2 mM Penicillium viridicatum
Cu2+ 67% inhibition at 2 mM Penicillium viridicatum
Fe3+ 85% inhibition at 5 mM Penicillium viridicatum
Hg2+ complete inhibition at 5 mM Penicillium viridicatum
K+ 37% inhibition at 2 mM, 16% inhibition at 10 mM Penicillium viridicatum
Mg2+ 73% inhibition at 2 mM Penicillium viridicatum
Mn2+ 10% inhibition at 10 mM, 10% activation at 2 mM Penicillium viridicatum
Ni2+ 37% inhibition at 2 mM, 19% inhibition at 10 mM Penicillium viridicatum
Zn2+ complete inhibition at 5 mM Penicillium viridicatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics in presence and absence of Ca2+, overview Penicillium viridicatum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ enhances the stability of enzyme, and its activity by 10% at 2 mM and 10 mM, and by 30% at 5 mM Penicillium viridicatum
Co2+ 7% inhibition at 10 mM, 7% activation at 2 mM Penicillium viridicatum
Mn2+ 10% inhibition at 10 mM, 10% activation at 2 mM Penicillium viridicatum
Na+ 23% activation at 2 mM, no effect at 10 mM Penicillium viridicatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
x * 92000, SDS-PAGE Penicillium viridicatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pectin + H2O Penicillium viridicatum the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification galacturonic acid + ?
-
?
pectin + H2O Penicillium viridicatum RFC3 the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification galacturonic acid + ?
-
?

Organism

Organism UniProt Comment Textmining
Penicillium viridicatum
-
isolated from decaying vegetables in Sao Jose do Rio Preto, SP, Brazil
-
Penicillium viridicatum RFC3
-
isolated from decaying vegetables in Sao Jose do Rio Preto, SP, Brazil
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 37.7fold from submerged fermentation to homogeneity Penicillium viridicatum

Source Tissue

Source Tissue Comment Organism Textmining
submerged culture
-
Penicillium viridicatum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
346.4
-
purified native enzyme Penicillium viridicatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apple pectin + H2O the activity on citrus pectin is appreciably higher than that on apple pectin Penicillium viridicatum galacturonic acid + ?
-
?
apple pectin + H2O the activity on citrus pectin is appreciably higher than that on apple pectin Penicillium viridicatum RFC3 galacturonic acid + ?
-
?
citric pectin + H2O the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification, the substrate shows 92% esterification Penicillium viridicatum galacturonic acid + ?
-
?
citric pectin + H2O the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification, the substrate shows 92% esterification Penicillium viridicatum RFC3 galacturonic acid + ?
-
?
pectin + H2O the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification Penicillium viridicatum galacturonic acid + ?
-
?
pectin + H2O the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification Penicillium viridicatum RFC3 galacturonic acid + ?
-
?

Subunits

Subunits Comment Organism
? x * 92000, SDS-PAGE Penicillium viridicatum

Synonyms

Synonyms Comment Organism
Exo-PG
-
Penicillium viridicatum
exo-polygalacturonase
-
Penicillium viridicatum
exopolygalacturonase
-
Penicillium viridicatum
PG III
-
Penicillium viridicatum
polymethylgalacturonase
-
Penicillium viridicatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55
-
Penicillium viridicatum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 70 profile, overview Penicillium viridicatum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5 55 purified enzyme, absence of substrate, 1 h, 10 mM Ca2+, 80-100% activity remaining Penicillium viridicatum
5 35 purified enzyme, absence of substrate, 1 h, 85-100% activity remaining Penicillium viridicatum
60
-
purified enzyme, loss of 50% activity in absence of Ca2+ after 25 min, in presence of 10 mM Ca2+ after 37 min Penicillium viridicatum
70
-
purified enzyme, absence of substrate, 1 h, 45% activity remaining, 70% in presence of 10 mM Ca2+ Penicillium viridicatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Penicillium viridicatum

pH Range

pH Minimum pH Maximum Comment Organism
4 8 50% of maximal activity at pH 8.0, profile, overview Penicillium viridicatum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 5 in presence of Ca2+, stable Penicillium viridicatum

pI Value

Organism Comment pI Value Maximum pI Value
Penicillium viridicatum isoelectric focusing
-
5.4