General Stability | Organism |
---|---|
Ca2+ enhances the stability of enzyme and its activity by 30% | Penicillium viridicatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 7% inhibition at 10 mM, 13% inhibition at 2 mM | Penicillium viridicatum | |
Al3+ | 33% inhibition at 2 mM, no inhibition at 10 mM | Penicillium viridicatum | |
Ba2+ | 10% inhibition at 2-5 mM | Penicillium viridicatum | |
Co2+ | 7% inhibition at 10 mM, 7% activation at 2 mM | Penicillium viridicatum | |
Cu2+ | 67% inhibition at 2 mM | Penicillium viridicatum | |
Fe3+ | 85% inhibition at 5 mM | Penicillium viridicatum | |
Hg2+ | complete inhibition at 5 mM | Penicillium viridicatum | |
K+ | 37% inhibition at 2 mM, 16% inhibition at 10 mM | Penicillium viridicatum | |
Mg2+ | 73% inhibition at 2 mM | Penicillium viridicatum | |
Mn2+ | 10% inhibition at 10 mM, 10% activation at 2 mM | Penicillium viridicatum | |
Ni2+ | 37% inhibition at 2 mM, 19% inhibition at 10 mM | Penicillium viridicatum | |
Zn2+ | complete inhibition at 5 mM | Penicillium viridicatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics in presence and absence of Ca2+, overview | Penicillium viridicatum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+ enhances the stability of enzyme, and its activity by 10% at 2 mM and 10 mM, and by 30% at 5 mM | Penicillium viridicatum | |
Co2+ | 7% inhibition at 10 mM, 7% activation at 2 mM | Penicillium viridicatum | |
Mn2+ | 10% inhibition at 10 mM, 10% activation at 2 mM | Penicillium viridicatum | |
Na+ | 23% activation at 2 mM, no effect at 10 mM | Penicillium viridicatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
92000 | - |
x * 92000, SDS-PAGE | Penicillium viridicatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | Penicillium viridicatum | the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification | galacturonic acid + ? | - |
? | |
pectin + H2O | Penicillium viridicatum RFC3 | the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification | galacturonic acid + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium viridicatum | - |
isolated from decaying vegetables in Sao Jose do Rio Preto, SP, Brazil | - |
Penicillium viridicatum RFC3 | - |
isolated from decaying vegetables in Sao Jose do Rio Preto, SP, Brazil | - |
Purification (Comment) | Organism |
---|---|
native enzyme 37.7fold from submerged fermentation to homogeneity | Penicillium viridicatum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
submerged culture | - |
Penicillium viridicatum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
346.4 | - |
purified native enzyme | Penicillium viridicatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
apple pectin + H2O | the activity on citrus pectin is appreciably higher than that on apple pectin | Penicillium viridicatum | galacturonic acid + ? | - |
? | |
apple pectin + H2O | the activity on citrus pectin is appreciably higher than that on apple pectin | Penicillium viridicatum RFC3 | galacturonic acid + ? | - |
? | |
citric pectin + H2O | the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification, the substrate shows 92% esterification | Penicillium viridicatum | galacturonic acid + ? | - |
? | |
citric pectin + H2O | the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification, the substrate shows 92% esterification | Penicillium viridicatum RFC3 | galacturonic acid + ? | - |
? | |
pectin + H2O | the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification | Penicillium viridicatum | galacturonic acid + ? | - |
? | |
pectin + H2O | the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification | Penicillium viridicatum RFC3 | galacturonic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 92000, SDS-PAGE | Penicillium viridicatum |
Synonyms | Comment | Organism |
---|---|---|
Exo-PG | - |
Penicillium viridicatum |
exo-polygalacturonase | - |
Penicillium viridicatum |
exopolygalacturonase | - |
Penicillium viridicatum |
PG III | - |
Penicillium viridicatum |
polymethylgalacturonase | - |
Penicillium viridicatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | - |
Penicillium viridicatum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 70 | profile, overview | Penicillium viridicatum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 55 | purified enzyme, absence of substrate, 1 h, 10 mM Ca2+, 80-100% activity remaining | Penicillium viridicatum |
5 | 35 | purified enzyme, absence of substrate, 1 h, 85-100% activity remaining | Penicillium viridicatum |
60 | - |
purified enzyme, loss of 50% activity in absence of Ca2+ after 25 min, in presence of 10 mM Ca2+ after 37 min | Penicillium viridicatum |
70 | - |
purified enzyme, absence of substrate, 1 h, 45% activity remaining, 70% in presence of 10 mM Ca2+ | Penicillium viridicatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Penicillium viridicatum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | 50% of maximal activity at pH 8.0, profile, overview | Penicillium viridicatum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | in presence of Ca2+, stable | Penicillium viridicatum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Penicillium viridicatum | isoelectric focusing | - |
5.4 |