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Literature summary for 3.2.1.67 extracted from
- Production, characterization and application of a thermostable exo-polygalacturonase by Bacillus subtilis CM5 (2010), Food Biotechnol., 24, 37-50.
No PubMed abstract available
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis CM5 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and DEAE cellulose column chromatography | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citric pectin + H2O | - |
Bacillus subtilis | D-galacturonic acid + ? | - |
? |  |
| citric pectin + H2O | - |
Bacillus subtilis CM5 | D-galacturonic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Exo-PG | - |
Bacillus subtilis |
| exo-polygalacturonase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | when the crude enzyme is heated at 60°C for 30 min, 80% of the original activity is lost, and at incubation at 70°C for 30 min., the enzyme activity is abolished | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bacillus subtilis |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Bacillus subtilis | ammonium chloride and urea (1%) inhibit enzyme production | down |
| Bacillus subtilis | the optimum temperature, pH, and incubation period for optimum enzyme production (82.0-83.2 units) are 50°C, 7.0, and 36 h, respectively. Bacillus subtilis produces more exo-PG in culture medium with peptone (1%) than other nitrogen sources such as beef extract, casein, yeast extract, ammonium sulfate, and ammonium acetate | up |
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