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Literature summary for 3.2.1.67 extracted from
- Partially purified polygalacturonase from Aspergillus niger (SA6) (2010), Afr. J. Biotechnol., 9, 8944-8954.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| stability studies of the enzyme at different pH values after 24 h of incubation at room temperature give the highest percentage residual activities as follows: 23.01% at pH 4.5 in acetate, 28.32% at pH 7.0 in phosphate, and 22.13% at pH 7.5 in Tris-HCl | Aspergillus niger |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
1 * 40000 + 1 * 35000, dimeric protein consisting of both endo- and exo-polygalacturonase activities, SDS-PAGE | Aspergillus niger |
| 40000 | - |
1 * 40000 + 1 * 35000, dimeric protein consisting of both endo- and exo-polygalacturonase activities, SDS-PAGE | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger SA6 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-75 gel filtration | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26.99 | - |
crude enzyme, at pH 4.5 and 40°C | Aspergillus niger |
| 246 | - |
after 9.1fold purification, at pH 4.5 and 40°C | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citric pectin + H2O | - |
Aspergillus niger | D-galacturonic acid + ? | - |
? |  |
| citric pectin + H2O | - |
Aspergillus niger SA6 | D-galacturonic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 40000 + 1 * 35000, dimeric protein consisting of both endo- and exo-polygalacturonase activities, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exoPG | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | - |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | about 55% activity at 30-40°C, about 45% activity at 50°C, about 25% activity at 60°C, about 15% activity at 70°C | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | - |
Aspergillus niger |
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Release 2023.1 (January 2023)
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