3.2.1.168: hesperidin 6-O-alpha-L-rhamnosyl-beta-D-glucosidase

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For detailed information about hesperidin 6-O-alpha-L-rhamnosyl-beta-D-glucosidase, go to the full flat file.

Reaction

Synonyms

6-O-alpha-rhamnosyl-beta-glucosidase, alpha-rhamnosyl-beta-glucosidase, alphaRbetaG, AMIS_8220, hesperidin deglycosylase, rutinase, rutinoside transferase

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.168 hesperidin 6-O-alpha-L-rhamnosyl-beta-D-glucosidase

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Results	in table
2	Activating Compound
5	AA Sequence
5	Application
1	Cloned(Commentary)
9	Expression
18	General Information
5	Inhibitors
2	kcat/KM [mM/s]
7	KM Value [mM]
11	Localization
12	Metals/Ions
2	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
17	Organism
8	pH Optimum
1	pH Range
1	pH Stability
2	pI Value
1	Posttranslational Modification
1	Protein Variants
5	Purification (Commentary)
3	Reaction
1	Reaction Type
17	Reference
9	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
77	Substrates and Products (Substrate)
16	Subunits
25	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
2	Turnover Number [1/s]

Engineering

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Engineering on EC 3.2.1.168 - hesperidin 6-O-alpha-L-rhamnosyl-beta-D-glucosidase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

additional information

Acremonium sp. DSM 24697

A0A286JZ59

immobilization of the enzyme by adsorption and crosslinking onto polyaniline-iron (PI) particles, method, overview. The immobilization yield and efficiency are relatively high, 31.2% and 8.9%, respectively. The heterogeneous preparation shows lower stability in comparison with the soluble form of the enzyme in operational conditions at 60°C due to protein metal-catalyzed oxidation achieved by iron traces supplied from the support. The harmful effect can be overcome by coating of the iron particles with polyethyleneimine (0.84 mg/g) previously to immobilization of the catalyst. The increased stability of the catalyst is correlated with the amount of iron released from the support. Under operational conditions, the uncoated particles lose between 76% and 52% activity after two cycles of reuse, whereas the PEI-coated preparation reduces 45-28% activity after five cycles of reuse in the range of pH 5.0-10, respectively

753040