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3.2.1.168: hesperidin 6-O-alpha-L-rhamnosyl-beta-D-glucosidase

This is an abbreviated version!
For detailed information about hesperidin 6-O-alpha-L-rhamnosyl-beta-D-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.168

Reaction

hesperidin
+
H2O
=
hesperetin
+
rutinose

Synonyms

6-O-alpha-rhamnosyl-beta-glucosidase, alpha-rhamnosyl-beta-glucosidase, alphaRbetaG, AMIS_8220, hesperidin deglycosylase, rutinase, rutinoside transferase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.168 hesperidin 6-O-alpha-L-rhamnosyl-beta-D-glucosidase

Purification

Purification on EC 3.2.1.168 - hesperidin 6-O-alpha-L-rhamnosyl-beta-D-glucosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme from culture supernatant to homogeneity by hydrophobic interaction and anion exchange chromatography
native enzyme from seeds by anion exchange chromatography and dialysis
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 Star (DE3)
to homogeneity, fractionation with ammonium sulfate and two chromatographic steps: butyl-agarose and QAE-Sephadex
-