3.2.1.163: 1,6-alpha-D-mannosidase
This is an abbreviated version!
For detailed information about 1,6-alpha-D-mannosidase, go to the full flat file.
Word Map on EC 3.2.1.163
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3.2.1.163
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caput
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synthesis
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alpha-mannosidases
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meishan
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mannosylation
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exoglycosidases
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1,4-dideoxy-1,4-imino-d-mannitol
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swainsonine
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gnrhr
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phoenicis
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epididymis-specific
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alpha-1,2-mannosidase
- 3.2.1.163
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caput
- synthesis
- alpha-mannosidases
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meishan
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mannosylation
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exoglycosidases
- 1,4-dideoxy-1,4-imino-d-mannitol
- swainsonine
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gnrhr
- phoenicis
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epididymis-specific
- alpha-1,2-mannosidase
Reaction
Hydrolysis of the (1->6)-linked alpha-D-mannose residues in alpha-D-Manp-(1->6)-D-Manp =
Synonyms
alpha 1,6-mannosidase, alpha-1,6-linkage-specific mannosidase, alpha-1,6-mannosidase, alpha-1,6-specific mannosidase, core-specific alpha1,6-mannosidase, core-specific lysosomal alpha (1-6)-mannosidase, core-specific lysosomal alpha 1,6-mannosidase, CpGH125, Epman, GH125 exo-alpha-1,6-mannosidase, lysosomal alpha (1-6)-mannosidase, lysosomal alpha 1,6-mannosidase, MAN2B2, More, SpGH125
ECTree
3.2.1.163 1,6-alpha-D-mannosidaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.2.1.163 - 1,6-alpha-D-mannosidase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranose
4-nitrophenyl alpha-D-mannopyranoside + H2O
4-nitrophenol + alpha-D-mannopyranose
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Epman is highly specific in its cleavage of the alpha-1,6 mannosidic linkage
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?
Man-alpha(1-3)[Man-alpha(l-6)]Man-beta(1-4)GlcNAc-beta(1-4)GlcNAc-beta(1-N)Asn + H2O
?
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i.e. Man3GlcNAc2Asn, degradation in concert with endoglycosidases, low activity, overview
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?
Man-alpha(1-6)Man-alpha(1-4)GlcNAc-beta(1-4)GlcNAc-beta(1-N)Asn + H2O
?
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i.e. Man2GlcNAc2Asn, degradation in concert with endoglycosidases, low activity, overview
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?
4-methylumbelliferyl alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranose
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synthesis of the fluorogenic disaccharide substrate and assay method development, overview
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?
4-methylumbelliferyl alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranose
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synthesis of the fluorogenic disaccharide substrate and assay method development, overview
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?
alpha-D-Manp-(1,6)-D-Manp + H2O
alpha-D-mannose
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the enzyme cleaves specifically (1-6)-linked mannobiose and has no activity towards alpha-D-Manp-1,2-D-Manp or alpha-D-Manp-1, 3-D-Manp
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?
alpha-D-Manp-(1,6)-D-Manp + H2O
alpha-D-mannose
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the enzyme cleaves specifically (1-6)-linked mannobiose and has no activity towards any other linkages
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?
additional information
?
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no activity with p-nitrophenyl-alpha-D-mannopyranoside or baker's yeast mannan
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?
additional information
?
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regioselective synthesis of mannobiose and mannotriose by reverse hydrolysis of commercially available manno-oligosaccharide
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?
additional information
?
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the core-specific lysosomal enzyme is involved in N-glycan catabolism and degradation of Man3GlcNAc2 N-glycan structures, requiring for chitobiase action before alpha1,6-mannosidase activity
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?
additional information
?
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the enzyme is active on the branched trimannosyl core of complex glycans cleaving alphal-6-linked mannose residues from branched Man3Glc-NAc to form Manalphal-3Manbeta1-4GlcNAc, overview, activities of alpha-mannosidases in fibroblasts, overview
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?
additional information
?
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substrate specificity, the enzyme cleaves alphal-6-linked mannose residues from branched Man3Glc-NAc to form Manalphal-3Manbeta1-4GlcNAc, but shows little activity on artificial substrates 4-methylumbelliferyl alpha-mannoside and 4-nitrophenyl alpha-mannoside, overview
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?
additional information
?
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the core-specific enzyme is specific for cleaving only the alpha1-6mannose residue from Man3GlcNAc but not Man3GlcNAc2 or other larger high mannose oligosaccharides, NMR analysis of substrates and products, overview
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?
additional information
?
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the core-specific lysosomal enzyme is involved in N-glycan catabolism, Man-alpha(l-6)linkage hydrolysis depends on the action of endoglycosidases, namely aspartyl-N-acetyl-beta-D-glucosaminidase and endo-N-acetyl-beta-D-glucosaminidase, overview
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?
additional information
?
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the enzyme does not possess the ability to decrease the extent of the O-mannose chains on glycoprotein A expressed in a GFI1.0 glycoengineered Pichia pastoris strain nor the ability to remove the single O-linked mannose residue
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?
additional information
?
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the recombinant enzyme shows no activity with recombinant human granulocyte colony-stimulating factor protein (rhGCSF) expressed in a GFI2.0 glycoengineered Pichia pastoris strain and possessing O-linked glycosylation composed of a single O-linked mannose residue. alpha-1,6-Mannosidase from Xanthomonas manihotis does not possess the ability to decrease the extent of the O-mannose chains
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?
