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Literature summary for 3.2.1.163 extracted from

  • Venkatesan, M.; Kuntz, D.; Rose, D.
    Human lysosomal alpha-mannosidases exhibit different inhibition and metal binding properties (2009), Protein Sci., 18, 2242-2251.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.163

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Drosophila S2 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(2R,3R,4S)-2-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidine-3,4-diol
-
 Homo sapiens
(3R,4R,5R)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)-1-methylpyrrolidin-2-one
-
 Homo sapiens
(3R,4R,5R)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidin-2-one
-
 Homo sapiens
Mannostatin A
-
 Homo sapiens
additional information Epman is not affected by 1 mM EDTA Homo sapiens
swainsonine
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3 4-nitrophenyl alpha-D-mannopyranoside in 100 mM sodium acetate at pH 4.0 and 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Epman is activated 3fold with 1 mM Co2+ Homo sapiens
Mg2+ Epman is stimulated 1.5fold with 1 mM Mg2+ Homo sapiens
Mn2+ Epman is stimulated 1.25fold with 1 mM Mn2+ Homo sapiens
Zn2+ contains Zn2+ in the active site, Epman is stimulated more than 1.5fold with 1 mM Zn2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 SDS-PAGE Homo sapiens
123600
-
 MALDI-TOF mass spectrometry Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Cibacron blue F3GA resin column chromatography, Sepharose column chromatography, and Superdex 200 gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0049
-
 culture supernatant, at pH 4.0 Homo sapiens
0.9
-
 after 182fold purification, at pH 4.0 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-mannopyranoside + H2O Epman is highly specific in its cleavage of the alpha-1,6 mannosidic linkage Homo sapiens 4-nitrophenol + alpha-D-mannopyranose
-
 ?

Synonyms

Synonyms Comment Organism
alpha-1,6-specific mannosidase
-
 Homo sapiens
Epman
-
 Homo sapiens
MAN2B2
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
3.6 5.5
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0006
-
 Mannostatin A in 100 mM sodium acetate at pH 4.0 and 37°C Homo sapiens
0.004
-
 swainsonine in 100 mM sodium acetate at pH 4.0 and 37°C Homo sapiens
0.026
-
 (3R,4R,5R)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)-1-methylpyrrolidin-2-one in 100 mM sodium acetate (pH 4.0), at 37°C Homo sapiens
0.21
-
 (3R,4R,5R)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidin-2-one in 100 mM sodium acetate (pH 4.0), at 37°C Homo sapiens
1.255
-
 (2R,3R,4S)-2-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidine-3,4-diol in 100 mM sodium acetate (pH 4.0), at 37°C Homo sapiens