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Literature summary for 3.2.1.163 extracted from
- The production, purification and characterization of two novel alpha-D-mannosidases from Aspergillus phoenicis (2005), Carbohydr. Res., 340, 609-617.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the organism is employed in regioselective synthesis of manno-oligosaccharides involving the enzyme | Aspergillus phoenicis |
General Stability
| General Stability | Organism |
|---|---|
| freeze and thaw cycles and freeze drying have a detrimental effect on the purified enzyme with loss of 60% activity | Aspergillus phoenicis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Aspergillus phoenicis |  |
| Mn2+ | strong inhibition | Aspergillus phoenicis | |
| additional information | no inhibition by EDTA at 10 mM | Aspergillus phoenicis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14 | - |
alpha-D-Manp-(1-6)-D-Manp | pH 4.0, 60°C | Aspergillus phoenicis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Ca2+ is not required for activity | Aspergillus phoenicis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
1 * 74000, SDS-PAGE | Aspergillus phoenicis |
| 81000 | - |
gel filtration | Aspergillus phoenicis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus phoenicis | - |
ATCC 14332, syn. Aspergillus saitoi | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 370fold by anion-exchange chromatography, chromatofocussing, and gel filtration | Aspergillus phoenicis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 48 | - |
purified enzyme | Aspergillus phoenicis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 23°C, room temperature, purified enzyme, over 95% remaining activity after 7 days | Aspergillus phoenicis |
| 4°C, purified enzyme, over 95% remaining activity after 3 months | Aspergillus phoenicis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-Manp-(1,6)-D-Manp + H2O | the enzyme cleaves specifically (1-6)-linked mannobiose and has no activity towards any other linkages | Aspergillus phoenicis | alpha-D-mannose | - |
? | |
| additional information | no activity with p-nitrophenyl-alpha-D-mannopyranoside or baker's yeast mannan | Aspergillus phoenicis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 74000, SDS-PAGE | Aspergillus phoenicis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Aspergillus phoenicis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
purified enzyme, stable for 24 h, rapid inactivation above | Aspergillus phoenicis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 4.5 | - |
Aspergillus phoenicis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 5 | 36% of maximal activity at pH 3.0, rapid inactivation above pH 5.0 | Aspergillus phoenicis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus phoenicis | isoelectric focusing | - |
4.6 |
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