3.2.1.163: 1,6-alpha-D-mannosidase

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For detailed information about 1,6-alpha-D-mannosidase, go to the full flat file.

Reaction

Hydrolysis of the (1->6)-linked alpha-D-mannose residues in alpha-D-Manp-(1->6)-D-Manp =

Synonyms

alpha 1,6-mannosidase, alpha-1,6-linkage-specific mannosidase, alpha-1,6-mannosidase, alpha-1,6-specific mannosidase, core-specific alpha1,6-mannosidase, core-specific lysosomal alpha (1-6)-mannosidase, core-specific lysosomal alpha 1,6-mannosidase, CpGH125, Epman, GH125 exo-alpha-1,6-mannosidase, lysosomal alpha (1-6)-mannosidase, lysosomal alpha 1,6-mannosidase, MAN2B2, More, SpGH125

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.163 1,6-alpha-D-mannosidase

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Results	in table
3	Application
1	CAS Registry Number
4	Cloned(Commentary)
5	Disease/ Diagnostics
3	General Information
1	General Stability
13	Inhibitors
1	kcat/KM [mM/s]
5	Ki Value [mM]
4	KM Value [mM]
4	Localization
9	Metals/Ions
5	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
11	Organism
8	pH Optimum
3	pH Range
1	pI Value
4	Purification (Commentary)
1	Reaction
11	Reference
3	Source Tissue
3	Specific Activity [micromol/min/mg]
2	Storage Stability
17	Substrates and Products (Substrate)
1	Subunits
17	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
1	Turnover Number [1/s]

Purification

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Purification on EC 3.2.1.163 - 1,6-alpha-D-mannosidase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

Cibacron blue F3GA resin column chromatography, Sepharose column chromatography, and Superdex 200 gel filtration

Homo sapiens

-

706628

native enzyme 370fold by anion-exchange chromatography, chromatofocussing, and gel filtration

Aspergillus phoenicis

-

664527

native enzyme partially by affinity chromatography

Homo sapiens

-

673892

recombinant His- or HA-tagged enzyme

Homo sapiens

-

674494