Literature summary for 3.2.1.163 extracted from

Deng, L.; Tsybina, P.; Gregg, K.; Mosi, R.; Zandberg, W.; Boraston, A.; Vocadlo, D.
Synthesis of 4-methylumbelliferyl alpha-D-mannopyranosyl-(1-6)-beta-D-mannopyranoside and development of a coupled fluorescent assay for GH125 exo-alpha-1,6-mannosidases (2013), Bioorg. Med. Chem., 21, 4839-4845.

Search for more literature for 3.2.1.163

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.38	-	4-methylumbelliferyl alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranoside	pH 7.4, 37°C	Clostridium perfringens

Organism

Organism	UniProt	Comment	Textmining
Clostridium perfringens	-	-	-
Streptococcus pneumoniae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranoside + H2O	synthesis of the fluorogenic disaccharide substrate and assay method development, overview	Streptococcus pneumoniae	4-methylumbelliferone + alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranose	-	?
4-methylumbelliferyl alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranoside + H2O	synthesis of the fluorogenic disaccharide substrate and assay method development, overview	Clostridium perfringens	4-methylumbelliferone + alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranose	-	?

Synonyms

Synonyms	Comment	Organism
CpGH125	-	Clostridium perfringens
GH125 exo-alpha-1,6-mannosidase	-	Streptococcus pneumoniae
GH125 exo-alpha-1,6-mannosidase	-	Clostridium perfringens
SpGH125	-	Streptococcus pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Clostridium perfringens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.9	-	4-methylumbelliferyl alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranoside	pH 7.4, 37°C	Clostridium perfringens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Clostridium perfringens

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the glycoside hydrolase family 125, GH125	Streptococcus pneumoniae
evolution	the enzyme belongs to the glycoside hydrolase family 125, GH125	Clostridium perfringens
physiological function	the enzyme is the pneumococcal virulence factor in Streptococcus pneumoniae	Streptococcus pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
12.9	-	4-methylumbelliferyl alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranoside	pH 7.4, 37°C	Clostridium perfringens

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Release 2023.1 (January 2023)