Literature summary for 3.2.1.163 extracted from

Park, C.; Meng, L.; Stanton, L.H.; Collins, R.E.; Mast, S.W.; Yi, X.; Strachan, H.; Moremen, K.W.
Characterization of a human core-specific lysosomal alpha 1,6-mannosidase involved in N-glycan catabolism (2005), J. Biol. Chem., 280, 37204-37216.

Search for more literature for 3.2.1.163

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as His- or HA-tagged protein	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
1,4-Dideoxy-1,4-imino-D-mannitol	-	Homo sapiens
swainsonine	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
lysosome	-	Homo sapiens	5764	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	stimulates	Homo sapiens
Zn2+	stimulates	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
180000	-	recombinant enzyme, gel filtration	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	the core-specific lysosomal enzyme is involved in N-glycan catabolism and degradation of Man3GlcNAc2 N-glycan structures, requiring for chitobiase action before alpha1,6-mannosidase activity	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	core-specific alpha1,6-mannosidase	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His- or HA-tagged enzyme	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	the enzyme is expressed in all tissues	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the core-specific lysosomal enzyme is involved in N-glycan catabolism and degradation of Man3GlcNAc2 N-glycan structures, requiring for chitobiase action before alpha1,6-mannosidase activity	Homo sapiens	?	-	?
additional information	the core-specific enzyme is specific for cleaving only the alpha1-6mannose residue from Man3GlcNAc but not Man3GlcNAc2 or other larger high mannose oligosaccharides, NMR analysis of substrates and products, overview	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
alpha 1,6-mannosidase	-	Homo sapiens
core-specific alpha1,6-mannosidase	-	Homo sapiens
core-specific lysosomal alpha 1,6-mannosidase	-	Homo sapiens
More	the enzyme belongs to the glycosylhydrolase family 38	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	acidic pH optimum	Homo sapiens

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Release 2023.1 (January 2023)