3.2.1.151: xyloglucan-specific endo-beta-1,4-glucanase

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For detailed information about xyloglucan-specific endo-beta-1,4-glucanase, go to the full flat file.

Word Map on EC 3.2.1.151

3.2.1.151

xyloglucans

tamarind

microfibrils

xxxg

expansins

wall-modifying

hemicellulosic

2.4.1.207

mixed-linkage

tropaeolum

nasturtium

agriculture

synthesis

myceliophthora

degradation

Reaction

= 2 xyloglucan oligosaccharides

Synonyms

AfXEG74, AspGD, AtXTH31, AtXTH32, bacterial glycoside hydrolase family 74 endo-xyloglucanase, BlXG12, CaXTH1, Cel74A, CJA_2477, CjGH74, DkXTH3, DkXTH4, DkXTH5, DkXTH6, DkXTH7, endo-1,4-beta-glucanase/xyloglucanase, endo-beta 1,4 xyloglucan hydrolase, endo-beta-1,4-(xylo)glucan hydrolase, endo-beta-1,4-xyloglucanase, endo-processive xyloglucanase, endo-xyloglucanase, endoxyloglucanase, family GH16 xyloglucan endo-transglycosylase, GH12 xyloglucanase, GH16 xyloglucan hydrolase, GH74 endo-xyloglucanase, GH74 xyloglucanase, Gly74A, GT77, LeXTH1, More, Mt-XTH1, MtXgh74, MYCTH_116384, NXG1, oligoxyloglucan hydrolase, PODO_RS11130, PoGH74, POXEG12A, PoxXEG12A, SaGH74A, SaGH74B, SIXTH5, TmNXG1, TXy, XcXGHA, XEG, Xeg1, XEG12A, XEG5, XEG5A, XEG74, XegA, XEH, XET, XG 25, XG 70, XG A, XG-ase, XG12, XG44, XG5, XGH74, Xgl74A, XH, XTH, XTH1, XTH15, XTH2, XTH3, XTH31, XTH4, XTH6, XTH7, xyloglucan endo-beta-1,4-glucanase, xyloglucan endohydrolase, xyloglucan endotransglucosylase/hydrolase, xyloglucan endotransglucosylase/hydrolase 3, xyloglucan endotransglucosylase/hydrolase protein 31, xyloglucan endotransglucosylase/hydrolase protein 32, xyloglucan endotransglucosylase/hydrolases, xyloglucan endotransglycosylase/hydrolase, xyloglucan hydrolase, xyloglucan-specific beta-(1,4)-glucanase, xyloglucan-specific endo 1,4-beta-glucanase, xyloglucan-specific endo-1,4beta-glucanase, xyloglucan-specific endo-beta-1,4-glucanase, xyloglucan-specific family 12 hydrolase, xyloglucanase, xyloglucanase 1, xyloglucanase A, xyloglucanendohydrolase, xyloglycan hydrolase

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.1.151 xyloglucan-specific endo-beta-1,4-glucanase

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Results	in table
7	Activating Compound
125	AA Sequence
7	Application
1	CAS Registry Number
45	Cloned(Commentary)
10	Crystallization (Commentary)
1	Expression
70	General Information
1	IC50 Value
30	Inhibitors
4	kcat/KM [mM/s]
2	Ki Value [mM]
33	KM Value [mM]
30	Localization
2	Metals/Ions
30	Molecular Weight [Da]
51	Natural Substrates/ Products (Substrates)
142	Organism
1	Pathway
71	PDB ID
45	pH Optimum
15	pH Range
12	pH Stability
20	pI Value
13	Posttranslational Modification
63	Protein Variants
23	Purification (Commentary)
1	Reaction
10	Reaction Type
124	Reference
93	Source Tissue
33	Specific Activity [micromol/min/mg]
242	Substrates and Products (Substrate)
45	Subunits
268	Synonyms
1	Systematic Name
29	Temperature Optimum [°C]
11	Temperature Range [°C]
21	Temperature Stability [°C]
43	Turnover Number [1/s]

Purification

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Purification on EC 3.2.1.151 - xyloglucan-specific endo-beta-1,4-glucanase

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5 entries

concentration with Centricon devices, cutoff 10 kDa

Tropaeolum majus

701134

endoI, endoIV and endoV

Trichoderma viride

646842

homogeneity

3 entries

native enzyme by ultrafiltration, dialysis, and gel filtration, recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis

Diospyros kaki

A0A067XSJ2, G5DAC8, G5DAC9

755080

native extracellular enzyme from culture filtrate by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration

Thermomonospora sp.

732248

native extracellular enzyme from culture medium by anion exchange chromatography

Penicillium canescens

707461

native extracellular enzyme from culture medium by anion exchange chromatography to over 95% purity

Talaromyces verruculosus

707461

partially recombinant extracellular enzyme from Pichia pastoris strains GS115 and recombinant enzyme expressed from mt74syn gene in Pichia pastoris strain VKPM Y-4269 by ethanol precipitation and dialysis

Thermothelomyces thermophilus

recombinant

recombinant GST-tagged enzyme from Escherichia coli BL21 by glutathione affinity chromatography and gel filtration

Daucus carota

Q05929

732055

recombinant His-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography

Penicillium oxalicum

A0A0A7RRF2

753871

recombinant His-tagged wild-type and mutant catalytic PoGH74cat enzyme modules from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, dialysis, and gel filtration

Paenibacillus odorifer

A0A1R0YRH0, A0A1R0ZNW0

752723

recombinant His-tagged wild-type enzyme, with a fusion of sfGFP to the C-terminus of carbohydrate binding module CBM10, and enzyme mutants from Escherichia coli strain Rosetta DE3 by nickel affinity chromatography

Cellvibrio japonicus

B3PKK9

753549

recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlus (DE3) RP by nickel affinity chromatography and gel filtration

Paenibacillus sp.

Q3MUH7

731816

recombinant N-terminally His6-tagged wild-type and mutant catalytic domains from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, ultrafiltration, and gel filtration

Paenibacillus odorifer

A0A1R0YRH0, A0A1R0ZNW0

752723