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Literature summary for 3.2.1.151 extracted from

  • Han, Y.; Zhu, Q.; Zhang, Z.; Meng, K.; Hou, Y.; Ban, Q.; Suo, J.; Rao, J.
    Analysis of xyloglucan endotransglycosylase/hydrolase (XTH) genes and diverse roles of isoenzymes during persimmon fruit development and postharvest softening (2015), PLoS ONE, 10, e0123668 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.151

Cloned(Commentary)

Cloned (Comment) Organism
gene XTH3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR expession analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Diospyros kaki
gene XTH4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR expession analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Diospyros kaki
gene XTH5, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR expession analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Diospyros kaki

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Diospyros kaki 5618
-
additional information the enzyme contains a signal peptide Diospyros kaki
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xyloglucan + H2O Diospyros kaki
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Diospyros kaki A0A067XSJ2 cv. Fupingjianshi, i.e. Diospyros chinensis
-
Diospyros kaki G5DAC8 cv. Fuping Jianshi, i.e. Diospyros chinensis
-
Diospyros kaki G5DAC9 cv. Fupingjianshi, i.e. Diospyros chinensis
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme contains a potential N-linked glycosylation (N-X-S/T) site Diospyros kaki

Purification (Commentary)

Purification (Comment) Organism
native enzyme by ultrafiltration, dialysis, and gel filtration, recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis Diospyros kaki

Source Tissue

Source Tissue Comment Organism Textmining
fruit expression analysis of isozymes DkXTH1-5 during Fuping Jianshi fruit development, expression patterns, overview Diospyros kaki
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tamarind xyloglucan + H2O 3% tamarind xyloglucan Diospyros kaki ?
-
 ?
xyloglucan + H2O
-
 Diospyros kaki ?
-
 ?

Subunits

Subunits Comment Organism
? x * 31930, sequence calculation Diospyros kaki
? x * 32340, sequence calculation Diospyros kaki
? x * 33530, sequence calculation Diospyros kaki

Synonyms

Synonyms Comment Organism
DkXTH3
-
 Diospyros kaki
DkXTH4
-
 Diospyros kaki
DkXTH5
-
 Diospyros kaki
More cf. EC 2.4.1.207 Diospyros kaki
XEH
-
 Diospyros kaki
XTH
-
 Diospyros kaki
xyloglucan endohydrolase
-
 Diospyros kaki
xyloglucan endotransglycosylase/hydrolase
-
 Diospyros kaki

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Diospyros kaki

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Diospyros kaki

pI Value

Organism Comment pI Value Maximum pI Value
Diospyros kaki sequence calculation
-
 5.36
Diospyros kaki sequence calculation
-
 6.45
Diospyros kaki sequence calculation
-
 8.94

General Information

General Information Comment Organism
evolution the XTH isozymes contain the conserved DEIDFEFLG motif identified as the catalytic domain of XTH Diospyros kaki
physiological function several XTH isozymes function in persimmon (Diospyros kaki) fruit development and postharvest softening. Isozymes DkXTH1, DkXTH4, and DkXTH5 expressions peak in immature expanding fruit, and their higher expression is observed along with higher fruit firmness in cold-treated fruit or firmer cultivar fruit during storage. The opposite gene expression patterns are observed in DkXTH2 and DkXTH3, which reach maxima concomitance with pronounced fruit softening. Meanwhile, the xyloglucan endotransglycosylase (XET, EC 2.4.1.207) enzymes play important roles in both the rapid growth and ripening of persimmon fruit. Furthermore, the recombined DkXTH1 and DkXTH2 proteins show significant XET activity without any detected XEH (EC 3.2.1.151) activity Diospyros kaki