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2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
3.8% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
-
?
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
100% activity
-
-
?
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O
?
-
-
-
-
?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + ?
-
1.3% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
-
?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
?
-
-
-
-
?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + ?
-
19.4% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
arabinoxyloglucan + H2O
arabinoxyloglucan oligosaccharides
-
-
-
-
?
azo-cellulose + H2O
azo-cellulose oligosaccharides
-
-
-
?
barley beta-glucan + H2O
?
barley-beta-glucan + H2O
?
beta-glucan + H2O
beta-glucan oligosaccharides
carboxyethyl cellulose + H2O
carboxyethyl cellulose oligosaccharides
-
-
-
-
?
carboxymethyl cellulose + H2O
?
carboxymethyl cellulose + H2O
carboxymethyl cellulose oligosaccharides
carboxymethyl curdlan + H2O
carboxymethyl curdlan oligosaccharides
-
less than 2% of the activity with xyloglucan
-
?
carboxymethyl pachyman + H2O
carboxymethyl pachyman oligosaccharides
-
less than 2% of the activity with xyloglucan
-
?
carboxymethylcellulose + H2O
?
carob galactomannan + H2O
?
-
4% activity compared to tamarind xyloglucan
-
-
?
cellohexaose + H2O
?
-
-
-
-
?
cellopentaose + H2O
?
-
-
-
-
?
cellotetraose + H2O
?
-
-
-
-
?
fucoxyloglucan + H2O
fucoxyloglucan oligosaccharides
-
-
-
-
?
galactoxyloglucan + H2O
?
Thermomonospora sp.
-
-
-
-
?
GGXXXG + H2O
Glc(beta1-4)Glc + XXXG
-
-
-
-
?
Glcbeta(1-3)Glcbeta(1-3)Glc + H2O
D-glucose + Glcbeta(1-3)Glc
-
-
38% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glc(1-4)Glc + H2O
D-glucose + Glcbeta(1-4)Glc
-
-
32% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glc(1-4)Glcbeta(1-4)Glc + H2O
Glcbeta(1-4)Glc
-
-
76% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glc-2-chloro-4-nitrophenol + H2O
D-glucose + 2-chloro-4-nitrophenyl-beta-D-glucoside
-
24% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
-
?
Glcbeta(1-4)Glcbeta(1-3)Glc + H2O
D-glucose + Glcbeta(1-3)Glc
-
-
52% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glcbeta(1-4)Glc(1-4)Glc-2-chloro-4-nitrophenol + H2O
Glcbeta(1-4)Glc(1-4)Glc + 2-chloro-4-nitrophenyl-beta-D-glucoside
-
-
84% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glcbeta(1-4)Glc-2-chloro-4-nitrophenol + H2O
Glcbeta(1-4)Glc + 2-chloro-4-nitrophenyl-beta-D-glucoside
-
-
68% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-3)Glc + H2O
Glcbeta(1-4)Glc + Glcbeta(1-3)Glc
-
-
83% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc + H2O
Glcbeta(1-4)Glcbeta(1-4)Glc + GG
-
-
-
-
?
Glcbeta(1->4)[Xylalpha(1->6)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
?
-
-
-
-
?
GXGXXXG + H2O
GXG + XXXG
-
-
-
-
?
hydroxyethyl cellulose + H2O
?
hydroxyethyl cellulose + H2O
hydroxyethyl cellulose oligosaccharides
-
-
-
-
?
laminarin + H2O
laminarin oligosaccharides
-
-
-
-
?
lichenan + H2O
lichenan cellulose oligosaccharides
-
-
-
-
?
octodecasaccharide XLLG-XLLG + H2O
?
-
xylogluco-nonasaccharide
-
-
?
pachyman + H2O
?
-
lower activity compared to xyloglucan
-
-
?
phosphoric acid-swollen cellulose + H2O
?
-
-
-
-
?
sulforhodamine-XLLG + H2O
?
tamarind seed xyloglucan + H2O
?
-
-
-
?
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc (1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-alpha-D-Xyl-(1->6)-beta-D-Glc-L-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc
tamarind seed xyloglucan + H2O
xyloglucooligosaccharides
tamarind seed xyloglucan + H2O
xylooligosaccharides
tamarind xyloglucan + H2O
?
tamarind xyloglucan + H2O
tamarind xyloglucooligosaccharides
XTH31 cleaves the substrate from about 186 kDa to about 6 kDa (median sizes) within 40 h. The enzyme does not have a preferred cleavage site close to either terminus
-
-
?
wheat arabinoxylan + H2O
?
-
15% activity compared to tamarind xyloglucan
-
-
?
XGXXXG + H2O
XG + XXXG
-
-
-
-
?
XXGXXXG + H2O
XXG + XXXG
-
-
-
-
?
XXXG + H2O
XX + XG
-
X stands for a substituted glucose residue, G for an unsubstituted glucose residue
270% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
XXXGXX + H2O
XXXG + XX
-
-
-
-
?
XXXGXXX + H2O
XXXG + XX
-
-
-
-
?
XXXGXXXG + H2O
XX + XGXX + XG
-
X stands for a substituted glucose residue, G for an unsubstituted glucose residue
hydrolysis at both chain ends of substrate. 390% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
XXXGXXXG + H2O
XXX + XXXG + GXXXG
XXXXGXXXXG + H2O
XXXXG
-
-
-
-
?
Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta + H2O
?
-
-
-
-
?
Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
?
-
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
xyloglucan + H2O
xyloglucooligosaccharides
xyloglucan heptasaccharide + H2O
?
tritium labeled
-
-
?
xyloglucan oligosaccharide + H2O
?
-
diverse substrates, substrate specificity, overview
-
-
?
xyloglucan oligosaccharide HDP-XGO + H2O
?
-
-
-
?
additional information
?
-
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
-
-
-
?
barley beta-glucan + H2O
?
-
lower activity compared to xyloglucan
-
-
?
barley beta-glucan + H2O
?
-
87% activity compared to tamarind xyloglucan
-
-
?
barley beta-glucan + H2O
?
4% activity compared to tamarind seed xyloglucan
-
-
?
barley beta-glucan + H2O
?
4% activity compared to tamarind seed xyloglucan
-
-
?
barley beta-glucan + H2O
?
4% activity compared to tamarind seed xyloglucan
-
-
?
barley-beta-glucan + H2O
?
approximately 50fold lower specific activity for the natural mixed-linkage (1->3)/(1->4)-beta-glucan from barley compared to xyloglucan
-
-
?
barley-beta-glucan + H2O
?
approximately 50fold lower specific activity for the natural mixed-linkage (1->3)/(1->4)-beta-glucan from barley compared to xyloglucan
-
-
?
beta-glucan + H2O
beta-glucan oligosaccharides
-
-
-
?
beta-glucan + H2O
beta-glucan oligosaccharides
-
-
-
-
?
carboxymethyl cellulose + H2O
?
enzyme CjGH74 shows approximately 165fold lower specific activity for the artificial polysaccharide derivative hydroxyethyl cellulose compared to xyloglucan
-
-
?
carboxymethyl cellulose + H2O
?
enzyme CjGH74 shows approximately 165fold lower specific activity for the artificial polysaccharide derivative hydroxyethyl cellulose compared to xyloglucan
-
-
?
carboxymethyl cellulose + H2O
?
-
-
-
?
carboxymethyl cellulose + H2O
carboxymethyl cellulose oligosaccharides
-
carboxymethyl cellulose-4M
-
-
?
carboxymethyl cellulose + H2O
carboxymethyl cellulose oligosaccharides
-
-
-
-
?
carboxymethyl cellulose + H2O
carboxymethyl cellulose oligosaccharides
-
-
-
-
?
carboxymethylcellulose + H2O
?
-
high activity
-
-
?
carboxymethylcellulose + H2O
?
2% activity compared to tamarind seed xyloglucan
-
-
?
carboxymethylcellulose + H2O
?
2% activity compared to tamarind seed xyloglucan
-
-
?
carboxymethylcellulose + H2O
?
2% activity compared to tamarind seed xyloglucan
-
-
?
hydroxyethyl cellulose + H2O
?
-
very low activity
-
-
?
hydroxyethyl cellulose + H2O
?
enzyme CjGH74 shows approximately 24fold lower specific activity for the artificial polysaccharide derivative hydroxyethyl cellulose compared to xyloglucan
-
-
?
hydroxyethyl cellulose + H2O
?
enzyme CjGH74 shows approximately 24fold lower specific activity for the artificial polysaccharide derivative hydroxyethyl cellulose compared to xyloglucan
-
-
?
hydroxyethyl cellulose + H2O
?
-
65% activity compared to tamarind xyloglucan
-
-
?
sulforhodamine-XLLG + H2O
?
-
-
-
?
sulforhodamine-XLLG + H2O
?
-
-
-
-
?
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc
-
-
-
?
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc
-
-
-
?
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc (1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-alpha-D-Xyl-(1->6)-beta-D-Glc-L-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc
-
-
-
?
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc (1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-alpha-D-Xyl-(1->6)-beta-D-Glc-L-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc
-
-
-
?
tamarind seed xyloglucan + H2O
xyloglucooligosaccharides
enzyme CjGH74 acting on tamarind XyG reveals that the catalytic module hydrolyzes the polysaccharide at unbranched backbone glucosyl residues to generate the oligosaccharides XXXG, XLXG, XXLG and XLLG, which differ in their degree of side-chain galactosylation. This is the most common cleavage pattern observed for GH74 endo-xyloglucanases
-
-
?
tamarind seed xyloglucan + H2O
xyloglucooligosaccharides
the enzyme specifically hydrolyzes tamarind xyloglucan in endo-acting mode
-
-
?
tamarind seed xyloglucan + H2O
xyloglucooligosaccharides
the enzyme specifically hydrolyzes tamarind xyloglucan in endo-acting mode
-
-
?
tamarind seed xyloglucan + H2O
xylooligosaccharides
best substrate, enzyme MtXgh74 hydrolyzes various linkages within the xyloglucan building blocks XXXG, XXLG, and XLXG (except XLLG) producing diverse low molecular weight oligosaccharides
-
-
?
tamarind seed xyloglucan + H2O
xylooligosaccharides
best substrate, enzyme MtXgh74 hydrolyzes various linkages within the xyloglucan building blocks XXXG, XXLG, and XLXG (except XLLG) producing diverse low molecular weight oligosaccharides
-
-
?
tamarind seed xyloglucan + H2O
xylooligosaccharides
best substrate, enzyme MtXgh74 hydrolyzes various linkages within the xyloglucan building blocks XXXG, XXLG, and XLXG (except XLLG) producing diverse low molecular weight oligosaccharides
-
-
?
tamarind xyloglucan + H2O
?
-
-
-
-
?
tamarind xyloglucan + H2O
?
-
-
-
-
?
tamarind xyloglucan + H2O
?
3% tamarind xyloglucan
-
-
?
tamarind xyloglucan + H2O
?
-
highest activity (100%)
-
-
?
tamarind xyloglucan + H2O
?
-
-
-
?
tamarind xyloglucan + H2O
?
-
-
-
?
tamarind xyloglucan + H2O
?
-
-
-
?
tamarind xyloglucan + H2O
?
high specific activity
-
-
?
XXXGXXXG + H2O
2 XXXG
-
-
-
?
XXXGXXXG + H2O
2 XXXG
-
-
-
?
XXXGXXXG + H2O
XXX + XXXG + GXXXG
-
-
-
?
XXXGXXXG + H2O
XXX + XXXG + GXXXG
-
-
-
?
xyloglucan + H2O
?
-
substrate specificity of fungal Xeg1 towards xyloglucan
-
-
?
xyloglucan + H2O
?
-
-
-
-
?
xyloglucan + H2O
?
-
xyloglucan endotransglucosylase/hydrolases are a family of enzymes that mediate the construction and restructuring of xyloglucan cross-links, thereby controlling the extensibility or mechanical properties of the cell wall in a wide variety of plant tissues
-
-
?
xyloglucan + H2O
?
-
-
-
-
?
xyloglucan + H2O
?
-
-
-
-
?
xyloglucan + H2O
?
-
-
-
?
xyloglucan + H2O
?
-
-
-
?
xyloglucan + H2O
?
the GH74 module (PoGH74cat) reveals a highly specific, processive endo-xyloglucanase activity that can hydrolyze the polysaccharide backbone at both branched and unbranched positions
-
-
?
xyloglucan + H2O
?
the GH74 module (PoGH74cat) reveals a highly specific, processive endo-xyloglucanase activity that can hydrolyze the polysaccharide backbone at both branched and unbranched positions
-
-
?
xyloglucan + H2O
?
-
-
-
?
xyloglucan + H2O
?
-
-
-
-
?
xyloglucan + H2O
?
-
-
-
-
?
xyloglucan + H2O
?
-
-
-
-
?
xyloglucan + H2O
?
-
-
-
-
?
xyloglucan + H2O
?
-
-
-
-
?
xyloglucan + H2O
?
-
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
source of substrate tamarind seed
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
xyloglucan endotransglucosylase/hydrolase proteins act at the microfibril-matrix interface during cell elongation
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
high specific activity towards tamarind xyloglucan
XXXG, XXLG and XLLG oligosaccharides
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
best substrate
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
substrate xyloglucan from tamarind seed, cleavage at unbranched glucose in the backbone
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
pea seed xyloglucan
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
tamarind and pea xyloglucan. The enzyme has at least four subsites (-2 to -2) and specifically recognizes xylose branching at the +1 and +2 sites, branching at the -1 site decreases the activity
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
xyloglucan endotransglucosylase/hydrolase proteins act at the microfibril-matrix interface during cell elongation
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
xyloglucan from tamarind seeds, structural analysis of xyloglucan binding structure with PoGH74cat, and structural basis of the bond cleavage pattern of PoGH74cat, overview
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
xyloglucan from tamarind seeds, structural analysis of xyloglucan binding structure with PoGH74cat, and structural basis of the bond cleavage pattern of PoGH74cat, overview
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
XEG5 randomly cleaves the xyloglucan main chain
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
XEG74 has dual endo-mode and exo-mode activities
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
XEG74 has dual endo-mode and exo-mode activities
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
XEG5 randomly cleaves the xyloglucan main chain
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
xyloglucan is a widespread hemicellulose polysaccharide of plant cell walls. It contains mainly D-glucose and D-xylose in the ratio of approximately 4:3, less amount of D-galactose, and also can contain L-fucose and L-arabinose
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
tamarind seed xyloglucan
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
source of substrate: tamarind seed
hydrolysis of unbranched glucose residues. Enzyme initially produces oligosaccharides with a degree of polymerization of 16-18, which are slowly hydrolyzed to final products with a degree of polymerization of 7-9. The ratio of the oligosaccharides with 7-9 to 16-18 glucose units is additionally dependent upon the pH value
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
specifically cleaves the 1,4-beta-glucosyl linkages of the xyloglucan backbone to yield mainly nona- and heptasaccharides
mainly nona- and heptasaccharides
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
xylem powder
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
decreases the weight-average molecular weight of xyloglucans
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
the enzyme depolymerizes xyloglucan more rapidly in the presence than in the absence of xyloglucan oligosaccharides, indicative of a endotransglucosylase activity
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
xyloglucan is a widespread hemicellulose polysaccharide of plant cell walls. It contains mainly D-glucose and D-xylose in the ratio of approximately 4:3, less amount of D-galactose, and also can contain L-fucose and L-arabinose
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
tamarind seed xyloglucan
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
hydrolysis at substituted glucose residues
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
tamarind seed xyloglucan
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
the enzyme catalyzes endohydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
decreases the weight-average molecular weight of xyloglucans
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
enzyme has hardly any effect on xyloglucans of less than 60 kDa
products are fragments of about 50 kDa, no production of any oligo- or monosaccharides
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
enzyme has no endo-type transglycosylation activity
products are fragments of 5 kDa, no production of oligo- or monosaccharides
?
xyloglucan + H2O
xyloglucooligosaccharides
-
-
-
?
xyloglucan + H2O
xyloglucooligosaccharides
the endo-xyloglucanase cleaves beta(1->4)-D-glucosidic linkages in the XyG backbone, high specific activity, strong preference for xyloglucan as a natural substrate
-
-
?
xyloglucan + H2O
xyloglucooligosaccharides
the endo-xyloglucanase cleaves beta(1->4)-D-glucosidic linkages in the XyG backbone, high specific activity
-
-
?
xyloglucan + H2O
xyloglucooligosaccharides
the endo-xyloglucanase cleaves beta(1->4)-D-glucosidic linkages in the XyG backbone, high specific activity, strong preference for xyloglucan as a natural substrate
-
-
?
additional information
?
-
-
enzyme does not cleave at substituted glucosyl moieties
-
-
?
additional information
?
-
-
enzyme is involved in protection of the plant against aphids
-
-
?
additional information
?
-
-
XEG activity affects the cell walls, overview
-
-
?
additional information
?
-
-
assay method development and evaluation for realtime detection and in vivo measurement of enzyme activity in plant tissue, overview. Substrate is a fluorogenic resorufin beta-glycoside of a xylogluco-oligosaccharide, isolated from Tamarindus indica seed xyloglucans and synthesized as a specific substrate for in planta analysis of XEH activity
-
-
?
additional information
?
-
enzyme XTH15, a classical group-I/II xyloglucan endotransglucosylase/hydrolase (XTH), has high xyloglucan endotransglucosylase (XET) and undetectable xyloglucan hydrolase (XEH) activity in vitro
-
-
?
additional information
?
-
enzyme XTH15, a classical group-I/II xyloglucan endotransglucosylase/hydrolase (XTH), has high xyloglucan endotransglucosylase (XET) and undetectable xyloglucan hydrolase (XEH) activity in vitro
-
-
?
additional information
?
-
hydrolysis products formed from [rt-3H]xyloglucan by XTH31 are analysed by GPC
-
-
?
additional information
?
-
hydrolysis products formed from [rt-3H]xyloglucan by XTH31 are analysed by GPC
-
-
?
additional information
?
-
-
no activity with beta-glucan and carboxymethylcellulose
-
-
?
additional information
?
-
no substrate: xylan
-
-
?
additional information
?
-
involved in plant cell wall polysaccharide degradation
-
-
?
additional information
?
-
no substrate: carboxymethyl cellulose-4M, xylan from birch, beech or oat spelt, lichenan, laminarin, pullulan, galactomannan, glucomannan
-
-
?
additional information
?
-
-
no substrate: carboxymethyl cellulose-4M, xylan from birch, beech or oat spelt, lichenan, laminarin, pullulan, galactomannan, glucomannan
-
-
?
additional information
?
-
-
modelling of substrate binding, overview. Substrate fucosylation does not affect the specific activity of this enzyme. AnXEG12A prefers xylogluco-oligosaccharides containing more than six glucose units, and with xylose substitution at the -3 and +1 subsites, branching structures do not significantly affect the hydrolytic efficiency of the enzyme, overview
-
-
?
additional information
?
-
substrate specificity, overview. The enzyme shows no endo-mannanase activity on guar galactomannan and konjac glucomannan, no endoxylanase activity on beechwood xylan and wheat flour arabinoxylan, and no endo-xanthanase on xanthan gum. The recombinant catalytic module indeed demonstrates a strong preference for XyG as a natural substrate. But CjGH74 is unable to release the chromophoric aglycones 2-chloro-4-nitrophenol (CNP) and resorufin from the artificial substrates, XXXG-beta-CNP and XXXG-beta-resorufin. No hydrolysis of the shorter chromogenic substrates CNP-beta-D-cellobioside (GG-beta-CNP) and CNP-beta-D-cellotrioside (GGG-beta-CNP). CjGH74 has an approximately 250 and 970fold higher specificity for xyloglucan compared to the artificial derivative hydroxyethyl cellulose and the mixed-linkage barley-beta-glucan, respectively
-
-
?
additional information
?
-
-
substrate specificity, overview. The enzyme shows no endo-mannanase activity on guar galactomannan and konjac glucomannan, no endoxylanase activity on beechwood xylan and wheat flour arabinoxylan, and no endo-xanthanase on xanthan gum. The recombinant catalytic module indeed demonstrates a strong preference for XyG as a natural substrate. But CjGH74 is unable to release the chromophoric aglycones 2-chloro-4-nitrophenol (CNP) and resorufin from the artificial substrates, XXXG-beta-CNP and XXXG-beta-resorufin. No hydrolysis of the shorter chromogenic substrates CNP-beta-D-cellobioside (GG-beta-CNP) and CNP-beta-D-cellotrioside (GGG-beta-CNP). CjGH74 has an approximately 250 and 970fold higher specificity for xyloglucan compared to the artificial derivative hydroxyethyl cellulose and the mixed-linkage barley-beta-glucan, respectively
-
-
?
additional information
?
-
substrate specificity, overview. The enzyme shows no endo-mannanase activity on guar galactomannan and konjac glucomannan, no endoxylanase activity on beechwood xylan and wheat flour arabinoxylan, and no endo-xanthanase on xanthan gum. The recombinant catalytic module indeed demonstrates a strong preference for XyG as a natural substrate. But CjGH74 is unable to release the chromophoric aglycones 2-chloro-4-nitrophenol (CNP) and resorufin from the artificial substrates, XXXG-beta-CNP and XXXG-beta-resorufin. No hydrolysis of the shorter chromogenic substrates CNP-beta-D-cellobioside (GG-beta-CNP) and CNP-beta-D-cellotrioside (GGG-beta-CNP). CjGH74 has an approximately 250 and 970fold higher specificity for xyloglucan compared to the artificial derivative hydroxyethyl cellulose and the mixed-linkage barley-beta-glucan, respectively
-
-
?
additional information
?
-
-
the enzyme encoded by CaXTH1 is involved in the cell expansion process of Cicer arietinum
-
-
?
additional information
?
-
-
no activity with carboxymethylcellulose, Avicel and barley 1,3-1,4-beta-glucan. NO activity with GXXXG and XXXGG. Low activity with GGGX and XXXGX
-
-
?
additional information
?
-
role in initiation and elongation of cotton fiber development
-
-
?
additional information
?
-
-
role in initiation and elongation of cotton fiber development
-
-
?
additional information
?
-
role in initiation and elongation of cotton fiber development
-
-
?
additional information
?
-
role in initiation and elongation of cotton fiber development
-
-
?
additional information
?
-
role in initiation and elongation of cotton fiber development
-
-
?
additional information
?
-
role in initiation and elongation of cotton fiber development
-
-
?
additional information
?
-
endo-xyloglucanases from GH74 can hydrolyze the regular structure of XXXG-type XyGs at the anomeric center of the unbranched glucosyl (G) unit, although some cleave the backbone at more sterically encumbered positions, e.g. between two X units
-
-
?
additional information
?
-
endo-xyloglucanases from GH74 can hydrolyze the regular structure of XXXG-type XyGs at the anomeric center of the unbranched glucosyl (G) unit, although some cleave the backbone at more sterically encumbered positions, e.g. between two X units
-
-
?
additional information
?
-
-
endo-xyloglucanases from GH74 can hydrolyze the regular structure of XXXG-type XyGs at the anomeric center of the unbranched glucosyl (G) unit, although some cleave the backbone at more sterically encumbered positions, e.g. between two X units
-
-
?
additional information
?
-
residue Gly476 is uniquely responsible for the promiscuous xyloglucan backbone-cleaving activity of the GH74 module PoGH74cat
-
-
?
additional information
?
-
residue Gly476 is uniquely responsible for the promiscuous xyloglucan backbone-cleaving activity of the GH74 module PoGH74cat
-
-
?
additional information
?
-
-
residue Gly476 is uniquely responsible for the promiscuous xyloglucan backbone-cleaving activity of the GH74 module PoGH74cat
-
-
?
additional information
?
-
enzymatic assays are performed on AZCL-xyloglucan, release of soluble dye fragments from cross-linked AZCL-XyG is measured. Enzyme PoGH74 shows essentially exclusive specificity for xyloglucan (XyG), which is typical for all GH74 members
-
-
-
additional information
?
-
enzymatic assays are performed on AZCL-xyloglucan, release of soluble dye fragments from cross-linked AZCL-XyG is measured. Enzyme PoGH74 shows essentially exclusive specificity for xyloglucan (XyG), which is typical for all GH74 members
-
-
-
additional information
?
-
-
enzymatic assays are performed on AZCL-xyloglucan, release of soluble dye fragments from cross-linked AZCL-XyG is measured. Enzyme PoGH74 shows essentially exclusive specificity for xyloglucan (XyG), which is typical for all GH74 members
-
-
-
additional information
?
-
enzymatic assays are performed on AZCL-xyloglucan, release of soluble dye fragments from cross-linked AZCL-XyG is measured. Enzyme PoGH74 shows essentially exclusive specificity for xyloglucan (XyG), which is typical for all GH74 members
-
-
-
additional information
?
-
enzymatic assays are performed on AZCL-xyloglucan, release of soluble dye fragments from cross-linked AZCL-XyG is measured. Enzyme PoGH74 shows essentially exclusive specificity for xyloglucan (XyG), which is typical for all GH74 members
-
-
-
additional information
?
-
endo-xyloglucanases from GH74 can hydrolyze the regular structure of XXXG-type XyGs at the anomeric center of the unbranched glucosyl (G) unit, although some cleave the backbone at more sterically encumbered positions, e.g. between two X units
-
-
?
additional information
?
-
endo-xyloglucanases from GH74 can hydrolyze the regular structure of XXXG-type XyGs at the anomeric center of the unbranched glucosyl (G) unit, although some cleave the backbone at more sterically encumbered positions, e.g. between two X units
-
-
?
additional information
?
-
residue Gly476 is uniquely responsible for the promiscuous xyloglucan backbone-cleaving activity of the GH74 module PoGH74cat
-
-
?
additional information
?
-
residue Gly476 is uniquely responsible for the promiscuous xyloglucan backbone-cleaving activity of the GH74 module PoGH74cat
-
-
?
additional information
?
-
-
no activity with 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta, cellotriose, and cellobiose
-
-
?
additional information
?
-
-
the enzyme demonstrates a broad substrate specificity for polysaccharides containing beta-1,4 linkages and displays an unusual specificity on defined xyloglucan oligosaccharides, cleaving the XXXG-XXXG repeat into XXX and GXXXG
-
-
?
additional information
?
-
no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan
-
-
?
additional information
?
-
no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan
-
-
?
additional information
?
-
-
no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan
-
-
?
additional information
?
-
analysis of xyloglucan digestion products by gel-filtration chromatography. Modelling of endo-dissociative- and endo-processive-type xyloglucanase structures, overview
-
-
?
additional information
?
-
analysis of xyloglucan digestion products by gel-filtration chromatography. Modelling of endo-dissociative- and endo-processive-type xyloglucanase structures, overview
-
-
?
additional information
?
-
no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan
-
-
?
additional information
?
-
no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan
-
-
?
additional information
?
-
-
the enzyme is organized in an enzyme complex
-
-
?
additional information
?
-
substrate specificity, the enzyme is highly specific for tamarind xyloglucan, it shows no activity against lichenan, methylcellulose, carboxymethyl cellulose, Avicel, xylan from beechwood, xylan from birch wood, barley glucan, laminarin, and galactan as substrates
-
-
?
additional information
?
-
-
substrate specificity, the enzyme is highly specific for tamarind xyloglucan, it shows no activity against lichenan, methylcellulose, carboxymethyl cellulose, Avicel, xylan from beechwood, xylan from birch wood, barley glucan, laminarin, and galactan as substrates
-
-
?
additional information
?
-
substrate specificity, the enzyme is highly specific for tamarind xyloglucan, it shows no activity against lichenan, methylcellulose, carboxymethyl cellulose, Avicel, xylan from beechwood, xylan from birch wood, barley glucan, laminarin, and galactan as substrates
-
-
?
additional information
?
-
-
very low activity against carboxymethyl cellulose, phosphoric acid-swollen cellulose, avicel, glucomannan, galactomannan, and xylan
-
-
?
additional information
?
-
-
no substrate: swollen cellulose
-
-
?
additional information
?
-
-
no substrate: (1--3,1--4)-beta-glucan
-
-
?
additional information
?
-
-
no substrate: carboxymethylcellulose
-
-
?
additional information
?
-
-
enzyme can act on isolated onion epidermis cell wall. Cell wall extension is significantly increased upon addition of enzyme to the isolated epidermis
-
-
?
additional information
?
-
-
involved in tissue softening of ripening fruits
-
-
?
additional information
?
-
-
SIXTH5 has no detectable effect on the mechanical properties of the wall
-
-
?
additional information
?
-
enzyme SaGH74A is endo-processive, hydrolysis pattern and mode of action, overview. The C-terminus of enzyme SaGH74A, which is annotated as a carbohydrate-binding module family 2 (CBM2), binds to beta-1,4-linked glucan-containing soluble polysaccharides such as hydroxyethyl cellulose, barley glucan, and xyloglucan, affinities, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
-
-
?
additional information
?
-
enzyme SaGH74A is endo-processive, hydrolysis pattern and mode of action, overview. The C-terminus of enzyme SaGH74A, which is annotated as a carbohydrate-binding module family 2 (CBM2), binds to beta-1,4-linked glucan-containing soluble polysaccharides such as hydroxyethyl cellulose, barley glucan, and xyloglucan, affinities, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
-
-
?
additional information
?
-
-
enzyme SaGH74A is endo-processive, hydrolysis pattern and mode of action, overview. The C-terminus of enzyme SaGH74A, which is annotated as a carbohydrate-binding module family 2 (CBM2), binds to beta-1,4-linked glucan-containing soluble polysaccharides such as hydroxyethyl cellulose, barley glucan, and xyloglucan, affinities, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
-
-
?
additional information
?
-
enzyme SaGH74B is a typical endo-enzyme, hydrolysis pattern and mode of action, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
-
-
?
additional information
?
-
enzyme SaGH74B is a typical endo-enzyme, hydrolysis pattern and mode of action, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
-
-
?
additional information
?
-
-
enzyme SaGH74B is a typical endo-enzyme, hydrolysis pattern and mode of action, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
-
-
?
additional information
?
-
enzyme SaGH74B is a typical endo-enzyme, hydrolysis pattern and mode of action, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
-
-
?
additional information
?
-
enzyme SaGH74A is endo-processive, hydrolysis pattern and mode of action, overview. The C-terminus of enzyme SaGH74A, which is annotated as a carbohydrate-binding module family 2 (CBM2), binds to beta-1,4-linked glucan-containing soluble polysaccharides such as hydroxyethyl cellulose, barley glucan, and xyloglucan, affinities, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
-
-
?
additional information
?
-
-
XG 25 and XG 70 are organized in an enzyme complex
-
-
?
additional information
?
-
-
no substrate: carboxymethylcellulose
-
-
?
additional information
?
-
enzyme MtXgh74 shows an endoprocessive mode of action. Oligosaccharides XXXG, XXLG, and XLXG are completely hydrolyzed at the end of the reaction, whereas XLLG oligosaccharide is not hydrolyzed
-
-
?
additional information
?
-
-
enzyme MtXgh74 shows an endoprocessive mode of action. Oligosaccharides XXXG, XXLG, and XLXG are completely hydrolyzed at the end of the reaction, whereas XLLG oligosaccharide is not hydrolyzed
-
-
?
additional information
?
-
enzyme MtXgh74 shows an endoprocessive mode of action. Oligosaccharides XXXG, XXLG, and XLXG are completely hydrolyzed at the end of the reaction, whereas XLLG oligosaccharide is not hydrolyzed
-
-
?
additional information
?
-
enzyme MtXgh74 shows an endoprocessive mode of action. Oligosaccharides XXXG, XXLG, and XLXG are completely hydrolyzed at the end of the reaction, whereas XLLG oligosaccharide is not hydrolyzed
-
-
?
additional information
?
-
-
nasturtium xyloglucanase 1, predominant endo-hydrolase of Tropaeolum majus that can also perform xyloglucan endo-transglycosylation at elevated substrate concentrations
-
-
?
additional information
?
-
-
assay method development and evaluation for realtime detection and in vivo measurement of enzyme activity in plant tissue, overview. Substrate is a fluorogenic resorufin beta-glycoside of a xylogluco-oligosaccharide, isolated from Tamarindus indica seed xyloglucans and synthesized as a specific substrate for in planta analysis of XEH activity
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-
?
additional information
?
-
the enzyme is devoid of transglycosylase activities. There is undetectable activity on cellulose, xylan, arbinoxylan, laminarin, or pectin
-
-
?
additional information
?
-
-
no substrate: (1--3,1--4)-beta-glucan
-
-
?
additional information
?
-
-
no substrate: carboxymethylcellulose
-
-
?
additional information
?
-
-
no substrate: hydroxyethyl cellulose
-
-
?
additional information
?
-
-
increases the capacity of the cell wall to extend
-
-
?
additional information
?
-
cleavage pattern of xyloglucan, overview
-
-
?
additional information
?
-
cleavage pattern of xyloglucan, overview
-
-
?
additional information
?
-
recombinant enzyme in vitro shows xyloglucan endotransglucosylase, but not xyloglucan hydrolase activity
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-
?
additional information
?
-
-
recombinant enzyme in vitro shows xyloglucan endotransglucosylase, but not xyloglucan hydrolase activity
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-
?
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0.01
-
substrate: xyloglucan, pH 5.0, 70°C
0.02
-
substrate: xyloglucan, pH 5.0, 50°C
0.1
-
substrate: xyloglucan, pH 5.0, 50°C
0.2
-
substrate: xyloglucan, pH 5.0, 70°C
0.5
-
substrate: xyloglucan, pH 5.0, 50°C
0.7
-
substrate: xyloglucan, pH 5.0, 70°C
0.75
-
substrate: xyloglucan, pH 5.0, 70°C
1.6
-
substrate: xyloglucan, pH 5.0, 50°C
11.9
purified recombinant enzyme, pH 5.5, 50°C, substrate XyGt
113
-
substrate pea seed xyloglucan
172
recombinant enzyme, substrate tamarind xyloglucan, pH 5.0, 60°C
21
-
purified XG A, substrate is tamarind xyloglucan
3 - 8
-
purified XG 70, substrate is tamarind xyloglucan
52
-
purified XG 25, substrate is tamarind xyloglucan
55
crude extracellular recombinant enzyme, pH 6.0, 60°C with 0.5% soluble or 1% insoluble polysaccharide substrate
80
partially purified recombinant enzyme, pH 6.0, 60°C with 0.5% soluble or 1% insoluble polysaccharide substrate
additional information
-
additional information
-
-
additional information
-
comparison with specific activities of other xyloglucanases towards polysaccharide substrates, overview
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH12 (group 2) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH12 (group 2) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH12 (group 2) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH12 (group 2) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH12 (group 2) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH12 (group 2) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH12 (group 2) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH12 (group 2) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH12 (group 2) highly expressed during fruit growth, decrease during ripening
additional information
-
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH12 (group 2) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH3 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH3 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH3 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH3 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH3 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH3 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH3 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH3 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH3 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
-
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH3 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH4 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH4 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH4 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH4 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH4 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH4 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH4 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH4 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH4 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
-
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH4 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH6 (group 3) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH6 (group 3) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH6 (group 3) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH6 (group 3) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH6 (group 3) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH6 (group 3) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH6 (group 3) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH6 (group 3) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH6 (group 3) highly expressed during fruit growth, decrease during ripening
additional information
-
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH6 (group 3) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH7 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH7 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH7 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH7 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH7 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH7 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH7 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH7 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH7 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
-
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH7 (group 1) highly expressed during fruit growth, decrease during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH8 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH8 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH8 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH8 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH8 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH8 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH8 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH8 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH8 (group 3) mainly expressed during ripening
additional information
-
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH8 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH9 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH9 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH9 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH9 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH9 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH9 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH9 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH9 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH9 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
-
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SIXTH9 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH2 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH2 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH2 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH2 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH2 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH2 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH2 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH2 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH2 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
-
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH2 (group 2) with generally low expression and no strong phase-dependent expression pattern
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH5 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH5 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH5 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH5 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH5 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH5 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH5 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH5 (group 3) mainly expressed during ripening
additional information
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH5 (group 3) mainly expressed during ripening
additional information
-
generally, highest transglucosylase activities (gene SIXTH1 - EC 2.4.1.207) during fruit growth, decrease during ripening, accordingly hydrolase activity decrease during ripening as indicated by xyloglucan viscosity decrease, expression of 10 different XTH genes in Solanum lycopersicum: SlXTH5 (group 3) mainly expressed during ripening
additional information
-
comparison with specific activities of other xyloglucanases towards polysaccharide substrates, overview
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evolution
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the enzyme belongs to the glucohydrolase family 12, GH12
evolution
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the enzyme belongs to the glycoside hydrolase family 12, GH12
evolution
the enzyme belongs to the glycoside hydrolase family 74, GH74
evolution
the enzyme is a member of GH family 12, GH12
evolution
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the enzyme belongs to the glycosyl hydrolase family 12, GH12
evolution
evolutionary relationship of persimmon DkXTH6 and DkXTH7 genes among other plant species and phylogenetic tree, overview. DkXTH6 and DkXTH7 possess several functional domains typical in plant XTHs, including the conserved amino acids (DEIDFEFLG) as a putative active site and together with a potential N-linked glycosylation (N-X-S/T) site
evolution
Paenibacillus odorifer produces a single multimodular enzyme containing a glycoside hydrolase (GH) family 74 module (AIQ73809). Endo-xyloglucanases, which catalyze the cleavage of the xyloglucans (XyGs) backbone (EC 3.2.1.151), are currently found in glycoside hydrolase (GH) families GH5, GH9, GH12, GH16, GH44, and GH74 (in CAZy classification). Of these, family GH74 is distinguished by fewer sequence members, an essentially singular specificity for xyloglucans, and a characteristic tertiary structure comprised of two 7-bladed beta-propeller domains that form a large interfacial cleft to accommodate the bulky polysaccharide
evolution
the enzyme belongs to the glycosyl hydrolase family 74, GH74. Myceliophthora thermophila strain VKPM F-244 produces two xyloglucan-degrading proteins with MWs of about 24 and 80 kDa. The 80 kDa protein is subjected to MALDI-TOF mass spectrometry peptide fingerprinting. Analysis of the data reveals maximal homology with the putative GH74 xyloglucanase from Myceliophthora thermophila ATCC 42464. The mtXgh74 gene encoding a GH74 xyloglucanase from strain VKPM F-244 is isolated using primers designed on the base of the MYCTH_116384 gene encoding AEO58927.1. The sequence of mtXgh74 is shown to be identical to MYCTH_116384
evolution
the enzyme belongs to the glycosyl hydrolase family 74, GH74. The CJA_2477 gene product comprises an N-terminal glycoside hydrolase family 74 (GH74) endo-xyloglucanase module in train with two carbohydrate-binding modules (CBMs) from families 10 and 2 (CBM10 and CBM2)
evolution
the XTH isozymes contain the conserved DEIDFEFLG motif identified as the catalytic domain of XTH
evolution
the endo-xyloglucanase belongs to the bacterial glycoside hydrolase family 74, GH74. Endo-xyloglucanases, which catalyze the cleavage of the XyGs backbone (EC 3.2.1.151), are currently found in glycoside hydrolase (GH) families GH5, GH9, GH12, GH16, GH44, and GH74 in the carbohydrate-active enzymes (CAZy) classification. Of these, family GH74 is distinguished by fewer sequence members, an essentially singular specificity for XyGs, and a characteristic tertiary structure comprised of two 7-bladed beta-propeller domains that form a large interfacial cleft to accommodate the bulky polysaccharide. Structure-activity relationships among characterized GH74 members, including determinants of endo versus exo (EC 3.2.1.150) activity, have been reviewed
evolution
the enzyme belongs to the endo-xyloglucanase family 74, GH74, family of enzymes. Phylogenetic diversity and catalytic mechanism of GH74 xyloglucanases from Ascomycete fungi, analysis of genome-wide distribution of GH74-encoding genes in ascomycetes, overview
evolution
-
the enzyme belongs to the glycosyl hydrolase family 74, GH74. Myceliophthora thermophila strain VKPM F-244 produces two xyloglucan-degrading proteins with MWs of about 24 and 80 kDa. The 80 kDa protein is subjected to MALDI-TOF mass spectrometry peptide fingerprinting. Analysis of the data reveals maximal homology with the putative GH74 xyloglucanase from Myceliophthora thermophila ATCC 42464. The mtXgh74 gene encoding a GH74 xyloglucanase from strain VKPM F-244 is isolated using primers designed on the base of the MYCTH_116384 gene encoding AEO58927.1. The sequence of mtXgh74 is shown to be identical to MYCTH_116384
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evolution
-
the enzyme belongs to the glucohydrolase family 12, GH12
-
evolution
-
the enzyme belongs to the endo-xyloglucanase family 74, GH74, family of enzymes. Phylogenetic diversity and catalytic mechanism of GH74 xyloglucanases from Ascomycete fungi, analysis of genome-wide distribution of GH74-encoding genes in ascomycetes, overview
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evolution
-
the enzyme belongs to the glycoside hydrolase family 74, GH74
-
evolution
-
the enzyme belongs to the glycosyl hydrolase family 74, GH74. Myceliophthora thermophila strain VKPM F-244 produces two xyloglucan-degrading proteins with MWs of about 24 and 80 kDa. The 80 kDa protein is subjected to MALDI-TOF mass spectrometry peptide fingerprinting. Analysis of the data reveals maximal homology with the putative GH74 xyloglucanase from Myceliophthora thermophila ATCC 42464. The mtXgh74 gene encoding a GH74 xyloglucanase from strain VKPM F-244 is isolated using primers designed on the base of the MYCTH_116384 gene encoding AEO58927.1. The sequence of mtXgh74 is shown to be identical to MYCTH_116384
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evolution
-
the enzyme belongs to the endo-xyloglucanase family 74, GH74, family of enzymes. Phylogenetic diversity and catalytic mechanism of GH74 xyloglucanases from Ascomycete fungi, analysis of genome-wide distribution of GH74-encoding genes in ascomycetes, overview
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evolution
-
the enzyme belongs to the endo-xyloglucanase family 74, GH74, family of enzymes. Phylogenetic diversity and catalytic mechanism of GH74 xyloglucanases from Ascomycete fungi, analysis of genome-wide distribution of GH74-encoding genes in ascomycetes, overview
-
evolution
-
the enzyme belongs to the endo-xyloglucanase family 74, GH74, family of enzymes. Phylogenetic diversity and catalytic mechanism of GH74 xyloglucanases from Ascomycete fungi, analysis of genome-wide distribution of GH74-encoding genes in ascomycetes, overview
-
evolution
-
the enzyme belongs to the glycoside hydrolase family 74, GH74
-
evolution
-
the endo-xyloglucanase belongs to the bacterial glycoside hydrolase family 74, GH74. Endo-xyloglucanases, which catalyze the cleavage of the XyGs backbone (EC 3.2.1.151), are currently found in glycoside hydrolase (GH) families GH5, GH9, GH12, GH16, GH44, and GH74 in the carbohydrate-active enzymes (CAZy) classification. Of these, family GH74 is distinguished by fewer sequence members, an essentially singular specificity for XyGs, and a characteristic tertiary structure comprised of two 7-bladed beta-propeller domains that form a large interfacial cleft to accommodate the bulky polysaccharide. Structure-activity relationships among characterized GH74 members, including determinants of endo versus exo (EC 3.2.1.150) activity, have been reviewed
-
evolution
-
Paenibacillus odorifer produces a single multimodular enzyme containing a glycoside hydrolase (GH) family 74 module (AIQ73809). Endo-xyloglucanases, which catalyze the cleavage of the xyloglucans (XyGs) backbone (EC 3.2.1.151), are currently found in glycoside hydrolase (GH) families GH5, GH9, GH12, GH16, GH44, and GH74 (in CAZy classification). Of these, family GH74 is distinguished by fewer sequence members, an essentially singular specificity for xyloglucans, and a characteristic tertiary structure comprised of two 7-bladed beta-propeller domains that form a large interfacial cleft to accommodate the bulky polysaccharide
-
evolution
-
the enzyme belongs to the glycosyl hydrolase family 74, GH74. The CJA_2477 gene product comprises an N-terminal glycoside hydrolase family 74 (GH74) endo-xyloglucanase module in train with two carbohydrate-binding modules (CBMs) from families 10 and 2 (CBM10 and CBM2)
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malfunction
the enzyme hydrolytic activity is essentially completely eliminated in AtXTH31/AtXTH32 double knockout lines. Despite the elimination of enzyme hydrolase activity in initiating root hairs in the xth31/xth32 mutant, root hair initiation nonetheless appears to be grossly unaffected
malfunction
replacement of catalytic Gly476 with Tyr, which is conserved in many GH74 members, results in exclusive hydrolysis of xyloglucan at unbranched glucose units. Likewise, systematic replacement of the hydrophobic platform residues constituting the positive subsites indicated their relative contributions to the processive mode of action. Specifically, W347 (+3 subsite) and W348 (+5 subsite) are essential for processivity, while W406 (+2 subsite) and Y372 (+6 subsite) are not strictly essential, but aid processivity
malfunction
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the enzyme hydrolytic activity is essentially completely eliminated in AtXTH31/AtXTH32 double knockout lines. Despite the elimination of enzyme hydrolase activity in initiating root hairs in the xth31/xth32 mutant, root hair initiation nonetheless appears to be grossly unaffected
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malfunction
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replacement of catalytic Gly476 with Tyr, which is conserved in many GH74 members, results in exclusive hydrolysis of xyloglucan at unbranched glucose units. Likewise, systematic replacement of the hydrophobic platform residues constituting the positive subsites indicated their relative contributions to the processive mode of action. Specifically, W347 (+3 subsite) and W348 (+5 subsite) are essential for processivity, while W406 (+2 subsite) and Y372 (+6 subsite) are not strictly essential, but aid processivity
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metabolism
the enzyme from the saprophytic Gram-negative bacterium catalyzes the first step of xyloglucan degradation. The GH74 catalytic domain generates Glc4-based xyloglucooligosaccharide (XyGO) substrates for downstream enzymes through an endo-dissociative mode of action
metabolism
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the enzyme from the saprophytic Gram-negative bacterium catalyzes the first step of xyloglucan degradation. The GH74 catalytic domain generates Glc4-based xyloglucooligosaccharide (XyGO) substrates for downstream enzymes through an endo-dissociative mode of action
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physiological function
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XEG has a dose-dependent effect on seedling growth, morphology, cell wall, and xyloglucan composition
physiological function
isoform XTH2 is associated with petal growth and development during carnation flower opening
physiological function
isoform XTH3 is associated with petal growth and development during carnation flower opening
physiological function
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XTH3 transgenic tomato plants exhibit a markedly increased tolerance to salt and drought stresses. Transgenic tomato plants exposed to a salt stress of 100 mM NaCl retain the chlorophyll in their leaves and shows normal root elongation. They also remain green and unwithered following exposure to 2 weeks of dehydration. Despite this increased stress tolerance, the transgenic tomato plants show no detectable phenotype defects
physiological function
xyloglucan endohydrolysis is a latent activity in the root cell wall remaining from the period of cell elongation (where, in particular, AtXTH31 transcript levels are high), which is not strictly essential for root hair initiation
physiological function
xyloglucan is a major component of the cell wall in most plants, and xyloglucan-specific endo-beta-1,4-glucanase digests the polysaccharide chains of plant cell walls. To protect the cell wall from these enzymes, plants secrete glycoside hydrolase inhibitor proteins
physiological function
a molecule of tamarind xyloglucan has a certain structural regularity: it is composed of 1,4-beta-D-glucotetraose units, where three glucosyl residues modified with a D-xylose via alpha-1,6-glycosidic bonds are followed by an unbranched glucosyl residue. The D-xyloses may be decorated with beta-1,2-D-galactosyl or to small amounts with alpha-1,2-L-arabinosyl residues. Degradation of xyloglucan requires a broad range of hydrolases with different activities and specificities, including extracellular Xgh74 endoxyloglucanase
physiological function
a role for enzyme XTH31 in acid growth. Isozymes XTH15 and XTH31 are both strongly expressed in young, rapidly growing organs, suggesting that they play roles in cell expansion. In vitro, XTH15 has very high transglucanase (endotransglucosylase, XET) but undetectable hydrolytic activity (glucanase, XEH). In contrast, XTH31 has very high XEH activity and only slight XET activity
physiological function
isozymes XTH15 and XTH31 are both strongly expressed in young, rapidly growing organs, suggesting that they play roles in cell expansion. In vitro, XTH15 has very high transglucanase (endotransglucosylase, XET) but undetectable hydrolytic activity (glucanase, XEH). In contrast, XTH31 has very high XEH activity and only slight XET activity
physiological function
several XTH isozymes function in persimmon (Diospyros kaki) fruit development and postharvest softening. Isozymes DkXTH1, DkXTH4, and DkXTH5 expressions peak in immature expanding fruit, and their higher expression is observed along with higher fruit firmness in cold-treated fruit or firmer cultivar fruit during storage. The opposite gene expression patterns are observed in DkXTH2 and DkXTH3, which reach maxima concomitance with pronounced fruit softening. Meanwhile, the xyloglucan endotransglycosylase (XET, EC 2.4.1.207) enzymes play important roles in both the rapid growth and ripening of persimmon fruit. Furthermore, the recombined DkXTH1 and DkXTH2 proteins show significant XET activity without any detected XEH (EC 3.2.1.151) activity
physiological function
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the fungal symbiont provides a single xyloglucanase (Xeg1) to its ant farmers by upregulating the expression of this protein in the inflated hyphal tips (gongylidia) that the ants ingest. Similar to other enzymes ingested this way, also Xeg1 is not digested but vectored to the fresh leaf-fragment pulp at the top of fungus gardens via ant fecal fluid. Xeg1 is 4-5 times more active in fecal fluid when ants ingest their normal fungal food, compared to a sucrose control diet, as expected when they cannot produce Xeg1 themselves. Ingestion of the enzyme by the ants for transfer to the fecal fluid is actively promoted by the fungal symbiont
physiological function
the holoparasitic angiosperm Cuscuta develops haustoria that enable it to feed on other plants, e.g. tomato plants. Cell wall modifications seem to be required in order for the parasite to successfully infect a host, changes to xyloglucan through the activity of xyloglucan endotransglucosylases/hydrolases (XTHs) are essential. On the other hand, XTH expression is also detected in resistant tomato upon an attack by Cuscuta, which suggests that both host and parasite use these enzymes in their arms race. Cell wall-modifying activities of XTHs during parasitization , overview. XTHs might function to make the host's resources accessible to Cuscuta. One of the defense responses of Solanum lycopersicum is the increased expression of LeXTH1, a gene encoding an XTH. The function of LeXTH1 might be to promote the elongation of tomato epidermal cells that takes place at the site of contact with the parasite. Another possibility is that LeXTH1 is deployed to reinforce the tomato cell walls, presumably as a remedy against the cell wall loosening activity of Cuscuta XTHs at the interface. Increased levels of xyloglucan degradation occur in the haustorium of Cuscuta reflexa and in the infected Solanum lycopersicum host plant
physiological function
-
the holoparasitic angiosperm Cuscuta develops haustoria that enable it to feed on other plants, e.g. tomato plants. Cell wall modifications seem to be required in order for the parasite to successfully infect a host, changes to xyloglucan through the activity of xyloglucan endotransglucosylases/hydrolases (XTHs) are essential. On the other hand, XTH expression is also detected in resistant tomato upon an attack by Cuscuta, which suggests that both host and parasite use these enzymes in their arms race. Cell wall-modifying activities of XTHs during parasitization, overview. XTHs might function to make the host's resources accessible to Cuscuta. At the onset of haustorium development, the swelling of the parasite stem facing the host plant is facilitated by Cuscuta XTHs that promote expansive cell growth through wall loosening. As the haustorium begins its host-invasive growth, XTHs secreted from the infection organ aid tissue penetration by loosening host cell walls. Upon reaching the vascular bundles of its host, the cell wall loosening activity of Cuscuta XTHs at the host-parasite interface enables parasite feeding through apoplastic sugar transfer and/or by promoting vascular tissue differentiation. To prevent exaggerated cell wall loosening under low turgor pressure, Cuscuta XTHs must also strengthen its own walls. When Cuscuta attempts to invade cultivated tomato by deploying wall loosening XTHs at the interface, the counteractive wall strengthening activity of host-encoded XTHs prevents the haustorium from entering the host plant. Model of the putative function of XTHs in the parasitization strategy of Cuscuta, increased levels of xyloglucan degradation occur in the haustorium of Cuscuta reflexa and in the infected Solanum lycopersicum host plant
physiological function
XTH, an important enzyme involved in xyloglucan metabolism, can function as a xyloglucan endotransglycosylase (XET, EC 2.4.1.207) and/or a xyloglucan endohydrolase (XEH, EC 3.2.1.151), with the former transferring one xyloglucan molecule fragment to another and the latter responsible for hydrolysis of one xyloglucan molecule. DkXTH7 is likely to be involved in cell wall assembly, special roles of isozyme XTH6 and XTH7 in persimmon fruit softening, overvie. The XTH gene family members each play a certain role in plant growth, fruit ripening, and fruit softening. During persimmon fruit postharvest softening,expression of isozymes DkXTH6 and DkXTH7 follows two opposing patterns
physiological function
XTH, an important enzyme involved in xyloglucan metabolism, can function as a xyloglucan endotransglycosylase (XET, EC 2.4.1.207) and/or a xyloglucan endohydrolase (XEH, EC 3.2.1.151), with the former transferring one xyloglucan molecule fragment to another and the latter responsible for hydrolysis of one xyloglucan molecule. Enzyme DkXTH6 might take part in cell wall restructuring, special roles of isozyme XTH6 and XTH7 in persimmon fruit softening, overview. The XTH gene family members each play a certain role in plant growth, fruit ripening, and fruit softening. During persimmon fruit postharvest softening,expression of isozymes DkXTH6 and DkXTH7 follows two opposing patterns
physiological function
endo-xyloglucanases from the GH74 family hydrolyze xyloglucans (XyGs). Enzymatic XyG hydrolysis increases cellulose digestibility, which underscores their structural importance in the plant cell wall. XyGs are also found as storage polysaccharides in some seeds, e.g. tamarind, and therefore represent important agricultural byproducts that have applications in the food, biomaterial and medical sectors. The endo-xyloglucanase can hydrolyze the regular structure of XXXG-type XyGs at the anomeric center of the unbranched glucosyl (G) unit, although some cleave the backbone at more sterically encumbered positions, e.g. between two X units
physiological function
enzyme AfXEG74 has no CBM modul and shows high specificity in xyloglucan hydrolysis. AfXEG74 does not cleave short xyloglucan oligosaccharides
physiological function
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a molecule of tamarind xyloglucan has a certain structural regularity: it is composed of 1,4-beta-D-glucotetraose units, where three glucosyl residues modified with a D-xylose via alpha-1,6-glycosidic bonds are followed by an unbranched glucosyl residue. The D-xyloses may be decorated with beta-1,2-D-galactosyl or to small amounts with alpha-1,2-L-arabinosyl residues. Degradation of xyloglucan requires a broad range of hydrolases with different activities and specificities, including extracellular Xgh74 endoxyloglucanase
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physiological function
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enzyme AfXEG74 has no CBM modul and shows high specificity in xyloglucan hydrolysis. AfXEG74 does not cleave short xyloglucan oligosaccharides
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physiological function
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xyloglucan endohydrolysis is a latent activity in the root cell wall remaining from the period of cell elongation (where, in particular, AtXTH31 transcript levels are high), which is not strictly essential for root hair initiation
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physiological function
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a molecule of tamarind xyloglucan has a certain structural regularity: it is composed of 1,4-beta-D-glucotetraose units, where three glucosyl residues modified with a D-xylose via alpha-1,6-glycosidic bonds are followed by an unbranched glucosyl residue. The D-xyloses may be decorated with beta-1,2-D-galactosyl or to small amounts with alpha-1,2-L-arabinosyl residues. Degradation of xyloglucan requires a broad range of hydrolases with different activities and specificities, including extracellular Xgh74 endoxyloglucanase
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physiological function
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isoform XTH3 is associated with petal growth and development during carnation flower opening
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physiological function
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isoform XTH2 is associated with petal growth and development during carnation flower opening
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physiological function
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enzyme AfXEG74 has no CBM modul and shows high specificity in xyloglucan hydrolysis. AfXEG74 does not cleave short xyloglucan oligosaccharides
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physiological function
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enzyme AfXEG74 has no CBM modul and shows high specificity in xyloglucan hydrolysis. AfXEG74 does not cleave short xyloglucan oligosaccharides
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physiological function
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enzyme AfXEG74 has no CBM modul and shows high specificity in xyloglucan hydrolysis. AfXEG74 does not cleave short xyloglucan oligosaccharides
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physiological function
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endo-xyloglucanases from the GH74 family hydrolyze xyloglucans (XyGs). Enzymatic XyG hydrolysis increases cellulose digestibility, which underscores their structural importance in the plant cell wall. XyGs are also found as storage polysaccharides in some seeds, e.g. tamarind, and therefore represent important agricultural byproducts that have applications in the food, biomaterial and medical sectors. The endo-xyloglucanase can hydrolyze the regular structure of XXXG-type XyGs at the anomeric center of the unbranched glucosyl (G) unit, although some cleave the backbone at more sterically encumbered positions, e.g. between two X units
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additional information
key amino acid residues for the endo-processive activity of GH74 xyloglucanase, an endo-processive xyloglucanase, are W318 and W319, found in the positive subsites. They are essential for processive degradation and are responsible for maintaining binding interactions with xyloglucan polysaccharide through a stacking effect, three-dimensional homology modelling, overview. The enzyme has four characteristic tryptophan residues (W61, W64, W318, and W319) around the active site cleft. Although W61 and W64 are dispensable for endo-processive xyloglucanase activity, these residues contribute slightly to endo-processive activity and/or substrate recognition
additional information
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structure homology modelling using Aspergillus niger endoglucanase structure, PDB ID 1KS5
additional information
three-dimensional structure modelling, overview
additional information
the substrate binding site of CjGH74 lies in an open cleft at the intersection of the N- and C-terminal domains. The catalytic residues, Asp70 (catalytic base) and Asp483 (catalytic acid), are located on opposite sides in the middle of this cleft. Three-dimensional structure of enzyme CjGH74 in complex with xyloglucooligosaccharides, overview
additional information
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the substrate binding site of CjGH74 lies in an open cleft at the intersection of the N- and C-terminal domains. The catalytic residues, Asp70 (catalytic base) and Asp483 (catalytic acid), are located on opposite sides in the middle of this cleft. Three-dimensional structure of enzyme CjGH74 in complex with xyloglucooligosaccharides, overview
additional information
mode of action of enzyme AfXEG74, overview. AfXEG74 hydrolyzes xyloglucan from tamarind (XyGt) by acting mainly on unbranched glucosyl residues (G), either before or after this position. In some cases, XX positions can also be attacked at specific points, for example when this motif is linked to the XG position that occurs in regions deprived of galactosyl branches
additional information
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mode of action of enzyme AfXEG74, overview. AfXEG74 hydrolyzes xyloglucan from tamarind (XyGt) by acting mainly on unbranched glucosyl residues (G), either before or after this position. In some cases, XX positions can also be attacked at specific points, for example when this motif is linked to the XG position that occurs in regions deprived of galactosyl branches
additional information
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structure homology modelling using Aspergillus niger endoglucanase structure, PDB ID 1KS5
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additional information
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three-dimensional structure modelling, overview
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additional information
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mode of action of enzyme AfXEG74, overview. AfXEG74 hydrolyzes xyloglucan from tamarind (XyGt) by acting mainly on unbranched glucosyl residues (G), either before or after this position. In some cases, XX positions can also be attacked at specific points, for example when this motif is linked to the XG position that occurs in regions deprived of galactosyl branches
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additional information
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mode of action of enzyme AfXEG74, overview. AfXEG74 hydrolyzes xyloglucan from tamarind (XyGt) by acting mainly on unbranched glucosyl residues (G), either before or after this position. In some cases, XX positions can also be attacked at specific points, for example when this motif is linked to the XG position that occurs in regions deprived of galactosyl branches
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additional information
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mode of action of enzyme AfXEG74, overview. AfXEG74 hydrolyzes xyloglucan from tamarind (XyGt) by acting mainly on unbranched glucosyl residues (G), either before or after this position. In some cases, XX positions can also be attacked at specific points, for example when this motif is linked to the XG position that occurs in regions deprived of galactosyl branches
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additional information
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mode of action of enzyme AfXEG74, overview. AfXEG74 hydrolyzes xyloglucan from tamarind (XyGt) by acting mainly on unbranched glucosyl residues (G), either before or after this position. In some cases, XX positions can also be attacked at specific points, for example when this motif is linked to the XG position that occurs in regions deprived of galactosyl branches
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additional information
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key amino acid residues for the endo-processive activity of GH74 xyloglucanase, an endo-processive xyloglucanase, are W318 and W319, found in the positive subsites. They are essential for processive degradation and are responsible for maintaining binding interactions with xyloglucan polysaccharide through a stacking effect, three-dimensional homology modelling, overview. The enzyme has four characteristic tryptophan residues (W61, W64, W318, and W319) around the active site cleft. Although W61 and W64 are dispensable for endo-processive xyloglucanase activity, these residues contribute slightly to endo-processive activity and/or substrate recognition
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additional information
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the substrate binding site of CjGH74 lies in an open cleft at the intersection of the N- and C-terminal domains. The catalytic residues, Asp70 (catalytic base) and Asp483 (catalytic acid), are located on opposite sides in the middle of this cleft. Three-dimensional structure of enzyme CjGH74 in complex with xyloglucooligosaccharides, overview
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x * 85000, sequence calculation, x * 100000, recombinant enzyme, SDS-PAGE
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x * 85000, sequence calculation, x * 100000, recombinant enzyme, SDS-PAGE
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x * 85000, sequence calculation, x * 100000, recombinant enzyme, SDS-PAGE
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x * 85000, sequence calculation, x * 100000, recombinant enzyme, SDS-PAGE
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x * 85000, sequence calculation, x * 100000, recombinant enzyme, SDS-PAGE
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x * 23300, calculated, mature protein, x * 32000, SDS-PAGE
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x * 80811, catalytic module, sequence calculation, x * 80812, catalytic module, mass spectrometry
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x * 80811, catalytic module, sequence calculation, x * 80812, catalytic module, mass spectrometry
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x * 30830, sequence calculation
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x * 31930, sequence calculation
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x * 32340, sequence calculation
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x * 33530, sequence calculation
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x * 33760, sequence calculation
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x * 105000, SDS-PAGE
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x * 40000, SDS-PAGE
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x * 25000, XG A, SDS-PAGE, x * 26154, XG A, sequence calculation
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x * 80000, SDS-PAGE, enzyme without C-terminal carbohydratebinding module domain, x * 130000, SDS-PAGE, enzyme with C-terminal carbohydratebinding module domain
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x * 78000, recombinant enzyme, SDS.PAGE
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x * 92000, recombinant enzyme, SDS-PAGE
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x * 78000, recombinant enzyme, SDS.PAGE
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x * 92000, recombinant enzyme, SDS-PAGE
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?
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x * 25000, XG 25, SDS-PAGE, x * 70000, XG 70, SDS-PAGE, XG 25 and XG 70 are organized in an enzyme complex
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x * 80000, recombinant glycosylated enzyme, SDS-PAGE, x * 77000, recombinant deglycosylated enzyme, SDS-PAGE
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x * 80000, recombinant glycosylated enzyme, SDS-PAGE, x * 77000, recombinant deglycosylated enzyme, SDS-PAGE
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?
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x * 80000, recombinant glycosylated enzyme, SDS-PAGE, x * 77000, recombinant deglycosylated enzyme, SDS-PAGE
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?
x * 45455, calculated from amino acid sequence
monomer or dimer
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x * 23500, about, sequence calculation, the enzyme is a predominantly a monomer at 60°C and is more active in its monomeric state
monomer or dimer
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x * 23500, about, sequence calculation, the enzyme is a predominantly a monomer at 60°C and is more active in its monomeric state
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additional information
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AnXEG12A structure homology modelling, overview. Catalytic residues are D100, E115, and E201, predicted substrate binding residues are W22, and W119, and residues that are predicted to influence substrate specificity in GH12 are xyloglucanases W7, V94, and G127. The SST insertion sequence is loacted at residues 130-132
additional information
modular architecture of the native enzyme: the full-length gene product is composed of a signal peptide, a GH74 catalytic domain and two carbohydrate binding modules: CBM10 and CBM2. The GH74, CBM10 and CBM2 modules are connected by serine rich linkers. Calculated molecular masses of the recombinant domains CjGH74-CBM10-CBM2, CjGH74-CBM10, CjGH74, CjCBM10-sfGFP, sfGFP-CjCBM2 and sfGFP are 105.9, 91.2, 80.8, 35.6, 40.7, and 27.8 kDa, respectively. The enzyme structure consists of two seven-bladed beta-propeller domains. Structure comparisons
additional information
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modular architecture of the native enzyme: the full-length gene product is composed of a signal peptide, a GH74 catalytic domain and two carbohydrate binding modules: CBM10 and CBM2. The GH74, CBM10 and CBM2 modules are connected by serine rich linkers. Calculated molecular masses of the recombinant domains CjGH74-CBM10-CBM2, CjGH74-CBM10, CjGH74, CjCBM10-sfGFP, sfGFP-CjCBM2 and sfGFP are 105.9, 91.2, 80.8, 35.6, 40.7, and 27.8 kDa, respectively. The enzyme structure consists of two seven-bladed beta-propeller domains. Structure comparisons
additional information
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modular architecture of the native enzyme: the full-length gene product is composed of a signal peptide, a GH74 catalytic domain and two carbohydrate binding modules: CBM10 and CBM2. The GH74, CBM10 and CBM2 modules are connected by serine rich linkers. Calculated molecular masses of the recombinant domains CjGH74-CBM10-CBM2, CjGH74-CBM10, CjGH74, CjCBM10-sfGFP, sfGFP-CjCBM2 and sfGFP are 105.9, 91.2, 80.8, 35.6, 40.7, and 27.8 kDa, respectively. The enzyme structure consists of two seven-bladed beta-propeller domains. Structure comparisons
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additional information
mature PoGH74 is composed of an N-terminal GH74 catalytic module in-train with three modules of unknown function (X2 domain, PFAM03442, and a carbohydrate-binding module (CBM) family 3), modular architecture of the native Paenibacillus odorifer AIQ73809 gene product, and GH74 enzymes structure comparisons, overview
additional information
mature PoGH74 is composed of an N-terminal GH74 catalytic module in-train with three modules of unknown function (X2 domain, PFAM03442, and a carbohydrate-binding module (CBM) family 3), modular architecture of the native Paenibacillus odorifer AIQ73809 gene product, and GH74 enzymes structure comparisons, overview
additional information
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mature PoGH74 is composed of an N-terminal GH74 catalytic module in-train with three modules of unknown function (X2 domain, PFAM03442, and a carbohydrate-binding module (CBM) family 3), modular architecture of the native Paenibacillus odorifer AIQ73809 gene product, and GH74 enzymes structure comparisons, overview
additional information
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mature PoGH74 is composed of an N-terminal GH74 catalytic module in-train with three modules of unknown function (X2 domain, PFAM03442, and a carbohydrate-binding module (CBM) family 3), modular architecture of the native Paenibacillus odorifer AIQ73809 gene product, and GH74 enzymes structure comparisons, overview
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additional information
MALDI-TOF mass spectrometry peptide fingerprinting, enzyme sequences comparisons
additional information
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MALDI-TOF mass spectrometry peptide fingerprinting, enzyme sequences comparisons
additional information
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MALDI-TOF mass spectrometry peptide fingerprinting, enzyme sequences comparisons
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additional information
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MALDI-TOF mass spectrometry peptide fingerprinting, enzyme sequences comparisons
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additional information
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importance of various loops lining the active site. Subtle differences leading to a tighter hydrogen bonding pattern on the negative, glycosyl donor, binding subsites, together with loop flexibility on the positive, glycosyl acceptor, binding subsites appear to favor hydrolysis over transglycosylation in GH16 xyloglucan-active enzymes
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D74A
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crystallization data in complex with oligosaccharide substrate
E155A
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the mutant acts as glucosynthase and can perform the condensation of xyloglucosyl fluorides, albeit at poor rates
D483A
site-directed mutagenesis, a catalytic acid mutant, the mutation causes loss of the enzymatic activity by more than 10 000fold compared to the wild-type enzyme
D70A
site-directed mutagenesis, the mutation causes loss of the enzymatic activity by more than 10 000fold compared to the wild-type enzyme
D483A
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site-directed mutagenesis, a catalytic acid mutant, the mutation causes loss of the enzymatic activity by more than 10 000fold compared to the wild-type enzyme
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D70A
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site-directed mutagenesis, the mutation causes loss of the enzymatic activity by more than 10 000fold compared to the wild-type enzyme
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W13A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
W13A/W28A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
W28A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
Y24A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
E358S
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nucleophile mutant
K129A/R156Y
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increased stability mutant
W318A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
W319A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
W61A
site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme
W64A
site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme
W318A
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site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
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W319A
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site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
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W61A
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site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme
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W64A
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site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme
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DELTAYNIIG
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loop deletion variant of isoform NXG1, structurally similar to the strict endo-transglycolase from Populus tremula x Populus tremuloides. Mutant has a greatly increased transglycosylation:hydrolysis ratio
E94A
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the GH16 xyloglucan hydrolase mutant of TmNXG1 acting as glycosynthase is capable of synthesizing XLLG-based xyloglucan oligosaccharides at rates feasible for preparative synthesis, thus providing an essential expansion of product ranges
D70A
site-directed mutagenesis in the PoGH74cat module at the catalytic base
D70A
site-directed mutagenesis, mutation of the catalytic base, the mutant turnover rate is similar to wild-type
G476Y
site-directed mutagenesis in the PoGH74cat module at the -1 subsite
G476Y
site-directed mutagenesis, mutation of the -1 subsite, the mutant turnover rate is similar to wild-type
W347A
site-directed mutagenesis in the PoGH74cat module at the +3 subsite
W347A
site-directed mutagenesis, mutation of the +3 subsite, kcat value is increased by 5fold compared to wild-type
W348A
site-directed mutagenesis in the PoGH74cat module at the +5 subsite
W348A
site-directed mutagenesis, mutation of the +5 subsite, the mutant turnover rate is similar to wild-type
W406A
site-directed mutagenesis in the PoGH74cat module at the +2 subsite
W406A
site-directed mutagenesis, mutation of the +2 subsite, the mutant turnover rate is similar to wild-type
Y372A
site-directed mutagenesis in the PoGH74cat module at the +6 subsite
Y372A
site-directed mutagenesis, mutation of the +6 subsite, the mutant turnover rate is similar to wild-type
D70A
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site-directed mutagenesis, mutation of the catalytic base, the mutant turnover rate is similar to wild-type
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D70A
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site-directed mutagenesis in the PoGH74cat module at the catalytic base
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G476Y
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site-directed mutagenesis, mutation of the -1 subsite, the mutant turnover rate is similar to wild-type
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G476Y
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site-directed mutagenesis in the PoGH74cat module at the -1 subsite
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W347A
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site-directed mutagenesis, mutation of the +3 subsite, kcat value is increased by 5fold compared to wild-type
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W347A
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site-directed mutagenesis in the PoGH74cat module at the +3 subsite
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Y372A
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site-directed mutagenesis, mutation of the +6 subsite, the mutant turnover rate is similar to wild-type
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Y372A
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site-directed mutagenesis in the PoGH74cat module at the +6 subsite
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additional information
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enzyme deletion mutant. Myzus persicae aphids settle preferentially on the mutant rather than on the wild-type. Ectopic expression of enzyme in the phloem is not sufficient to confer protection
additional information
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loss-of-function mutant of isoform XTH21. Compared with wild type, root hairs and root cells of xth21 are shorter and cell shapes are anomalous
additional information
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atxth28: null mutant with insert blocking gene expression of AtXTH28, shorter proximal siliques with fewer seeds, reduced self-pollination, atxth28/AtXTH28: transgenic mutant with insertion of intact AtXTH28 fragment into atxth28 null-mutant, wild type silique growth and self-pollination ability restored, atxth27/atxth28: double-null-mutant, no additional phenotype expression compared to atxth28 null-mutant
additional information
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construction of random XEG T-DNA insertion mutants, dozens of plant cell wall xeg mutants are identified, leading to the identification of 23 genetic loci that affect plant cell walls. One of the identified loci is XEG113, encoding a family 77 glycosyltransferase, GT77. Detailed analysis of the wall of this mutant indicates that its extensins, structural glyocoproteins present in walls, are underarabinosylated. Xeg-113 plants exhibit more elongated hypocotyls than wild-type, mutant cell wall composition and phenotype, overview
additional information
contruction of XTH31 single and double knockout lines with gene XTH32, the enzyme hydrolytic activity is essentially completely eliminated in AtXTH31/AtXTH32 double knockout lines
additional information
contruction of XTH31 single and double knockout lines with gene XTH32, the enzyme hydrolytic activity is essentially completely eliminated in AtXTH31/AtXTH32 double knockout lines
additional information
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contruction of XTH31 single and double knockout lines with gene XTH32, the enzyme hydrolytic activity is essentially completely eliminated in AtXTH31/AtXTH32 double knockout lines
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additional information
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mutation and engineering of wild-type xyloglucanase to glycosynthases, hydrolytically inactive mutant glycosidases that catalyze glycosylation reactions using glycosyl fluoride donor substrates
additional information
transgenic expression in Arabidopsis thaliana leads to abnormal leaf morphology with twisting and bending along the edges. Leaves show increased numbers of small-sized cells, resulting in disordered, highly populated mesophyll cells in each dorsoventral layer containing a limited amount of starch. Transgenic plants display a markedly improved tolerance to severe water deficit, and to a lesser extent to high salinity in comparison with the wild-type plants
additional information
the full-length CJA_2477 gene product is composed of a signal peptide, a GH74 catalytic domain and two carbohydrate binding modules: CBM10 and CBM2. The GH74, CBM10 and CBM2 modules are connected by serine rich linkers. Construction of truncated enzyme forms and isolated domains and recombinant expression as His- and GFP-tagged proteins in Escherichia coli
additional information
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the full-length CJA_2477 gene product is composed of a signal peptide, a GH74 catalytic domain and two carbohydrate binding modules: CBM10 and CBM2. The GH74, CBM10 and CBM2 modules are connected by serine rich linkers. Construction of truncated enzyme forms and isolated domains and recombinant expression as His- and GFP-tagged proteins in Escherichia coli
additional information
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the full-length CJA_2477 gene product is composed of a signal peptide, a GH74 catalytic domain and two carbohydrate binding modules: CBM10 and CBM2. The GH74, CBM10 and CBM2 modules are connected by serine rich linkers. Construction of truncated enzyme forms and isolated domains and recombinant expression as His- and GFP-tagged proteins in Escherichia coli
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additional information
generation of PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675) truncated mutants
additional information
generation of PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675) truncated mutants
additional information
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generation of PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675) truncated mutants
additional information
construction of truncated mutants, i.e. PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675), the mutant turnover rate is similar to wild-type
additional information
construction of truncated mutants, i.e. PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675), the mutant turnover rate is similar to wild-type
additional information
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construction of truncated mutants, i.e. PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675), the mutant turnover rate is similar to wild-type
additional information
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construction of truncated mutants, i.e. PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675), the mutant turnover rate is similar to wild-type
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additional information
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generation of PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675) truncated mutants
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additional information
selective replacement of the positive subsite residues with alanine mutations reduces the degree of processive activity and resulted in the more endo-dissociative-activity
additional information
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selective replacement of the positive subsite residues with alanine mutations reduces the degree of processive activity and resulted in the more endo-dissociative-activity
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additional information
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overexpression of the enzyme in poplar trees leads to xylem and xyloglucan degradation accelerating cellulose saccharification. The crystalline region of the cellulose microfibrils is highly degraded in the xylem overexpressing xyloglucanase comapred to overexpression of cellulase, xylanase, or galactanase, overview
additional information
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TmNXG1-deltaYNIIG: active site loop deletion mutant, three loops: Asn84-Asp93 (glycosyl donor subsite), Glu117-Gly126, and Trp190-Tyr197 (critical to acceptor substrate binding, inter alia influencing transglycosylation/hydrolysis ratio)
additional information
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construction of a loop mutant TmNXG1-DELTAYNIIG
additional information
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mutation and engineering of wild-type xyloglucanase to glycosynthases, hydrolytically inactive mutant glycosidases that catalyze glycosylation reactions using glycosyl fluoride donor substrates
additional information
Arabidopsis thaliana plants expressing the enzyme show slightly increased xyloglucan endohydrolase activity and alterations in the cell wall structure and composition
additional information
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Arabidopsis thaliana plants expressing the enzyme show slightly increased xyloglucan endohydrolase activity and alterations in the cell wall structure and composition
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Hasper, A.A.; Dekkers, E.; Van Mil, M.; Van de Vondervoort, P.J.I.; De Graaff, L.H.
EglC, a new endoglucanase from Aspergillus niger with major activity towards xyloglucan
Appl. Environ. Microbiol.
68
1556-1560
2002
Aspergillus niger (Q8TFP1)
brenda
Matsumoto, T.; Sakai, F.; Hayashi, T.
A xyloglucan-specific endo-1,4-beta-glucanase isolated from auxin-treated pea stems
Plant Physiol.
114
661-667
1997
Pisum sativum
brenda
Kaku, T.; Tabuchi, A.; Wakabayashi, K.; Kamisaka, S.; Hoson, T.
Action of xyloglucan hydrolase within the native cell wall architecture and its effect on cell wall extensibility in azuki bean epicotyls
Plant Cell Physiol.
43
21-26
2002
Vigna angularis
brenda
Tabuchi, A.; Kamisaka, S.; Hoson, T.
Purification of xyloglucan hydrolase/endotransferase from cell walls of azuki bean epicotyls
Plant Cell Physiol.
38
653-658
1997
Vigna angularis
-
brenda
Pauly, M.; Andersen, L.N.; Kauppinen, S.; Kofod, L.V.; York, W.S.; Albersheim, P.; Darvill, A.
A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme
Glycobiology
9
93-100
1999
Aspergillus aculeatus (O94218), Aspergillus aculeatus
brenda
Rejon-Palomares, A.; Garcia-Garrido, J.M.; Ocampo, J.A.; Garcia-Romera, I.
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Aspergillus japonicus
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Vigna angularis
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Medicago truncatula
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Arabidopsis thaliana, Nicotiana tabacum
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The cell wall-modifying xyloglucan endotransglycosylase/hydrolase LeXTH1 is expressed during the defense reaction of tomato against the plant parasite Cuscuta reflexa
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Solanum lycopersicum
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Solanum lycopersicum
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Cicer arietinum
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Populus tremula x Populus tremuloides
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Cho, S.K.; Kim, J.E.; Park, J.A.; Eom, T.J.; Kim, W.T.
Constitutive expression of abiotic stress-inducible hot pepper CaXTH3, which encodes a xyloglucan endotransglucosylase/hydrolase homolog, improves drought and salt tolerance in transgenic Arabidopsis plants
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Capsicum annuum (Q1W4A1)
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Jimenez, T.; Martin, I.; Labrador, E.; Dopico, B.
The immunolocation of a xyloglucan endotransglucosylase/hydrolase specific to elongating tissues in Cicer arietinum suggests a role in the elongation of vascular cells
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Cicer arietinum
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Baumann, M.J.; Ekloef, J.M.; Michel, G.; Kallas, A.M.; Teeri, T.T.; Czjzek, M.; Brumer, H.
Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: Biological implications for cell wall metabolism
Plant Cell
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Tropaeolum majus
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Divol, F.; Vilaine, F.; Thibivilliers, S.; Kusiak, C.; Sauge, M.H.; Dinant, S.
Involvement of the xyloglucan endotransglycosylase/hydrolases encoded by celery XTH1 and Arabidopsis XTH33 in the phloem response to aphids
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Apium graveolens, Arabidopsis thaliana
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Nishikubo, N.; Awano, T.; Banasiak, A.; Bourquin, V.; Ibatullin, F.; Funada, R.; Brumer, H.; Teeri, T.T.; Hayashi, T.; Sundberg, B.; Mellerowicz, E.J.
Xyloglucan endo-transglycosylase (XET) functions in gelatinous layers of tension wood fibers in poplar - A glimpse into the mechanism of the balancing act of trees
Plant Cell Physiol.
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Populus sp.
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Van Sandt, V.S.; Suslov, D.; Verbelen, J.P.; Vissenberg, K.
Xyloglucan endotransglucosylase activity loosens a plant cell wall
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Selaginella kraussiana
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Master, E.R.; Zheng, Y.; Storms, R.; Tsang, A.; Powlowski, J.
A xyloglucan-specific family 12 glycosyl hydrolase from Aspergillus niger: recombinant expression, purification and characterization
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Aspergillus niger (A1XP58), Aspergillus niger
brenda
Desmet, T.; Cantaert, T.; Gualfetti, P.; Nerinckx, W.; Gross, L.; Mitchinson, C.; Piens, K.
An investigation of the substrate specificity of the xyloglucanase Cel74A from Hypocrea jecorina
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Trichoderma reesei
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Ishida, T.; Yaoi, K.; Hiyoshi, A.; Igarashi, K.; Samejima, M.
Substrate recognition by glycoside hydrolase family 74 xyloglucanase from the basidiomycete Phanerochaete chrysosporium
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Phanerodontia chrysosporium
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Liu, Y.; Liu, D.; Zhang, H.; Gao, H.; Guo, X.; Wang, D.; Zhang, X.; Zhang, A.
The alpha- and beta-expansin and xyloglucan endotransglucosylase/hydrolase gene families of wheat: molecular cloning, gene expression, and EST data mining
Genomics
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Triticum aestivum (Q56TP0), Triticum aestivum (Q56TP1), Triticum aestivum (Q56TP2), Triticum aestivum (Q56TP3), Triticum aestivum (Q56TP4), Triticum aestivum
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Miedes, E.; Lorences, E.P.
The implication of xyloglucan endotransglucosylase/hydrolase (XTHs) in tomato fruit infection by Penicillium expansum Link. A
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Solanum lycopersicum
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Martinez-Fleites, C.; Guerreiro, C.I.; Baumann, M.J.; Taylor, E.J.; Prates, J.A.; Ferreira, L.M.; Fontes, C.M.; Brumer, H.; Davies, G.J.
Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A, reveal the structural basis for xyloglucan recognition and degradation
J. Biol. Chem.
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Acetivibrio thermocellus
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Gloster, T.M.; Ibatullin, F.M.; Macauley, K.; Ekloef, J.M.; Roberts, S.; Turkenburg, J.P.; Bjornvad, M.E.; Jorgensen, P.L.; Danielsen, S.; Johansen, K.S.; Borchert, T.V.; Wilson, K.S.; Brumer, H.; Davies, G.J.
Characterization and three-dimensional structures of two distinct bacterial xyloglucanases from families GH5 and GH12
J. Biol. Chem.
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Bacillus licheniformis, Paenibacillus pabuli
brenda
Genovesi, V.; Fornale, S.; Fry, S.C.; Ruel, K.; Ferrer, P.; Encina, A.; Sonbol, F.M.; Bosch, J.; Puigdomenech, P.; Rigau, J.; Caparros-Ruiz, D.
ZmXTH1, a new xyloglucan endotransglucosylase/hydrolase in maize, affects cell wall structure and composition in Arabidopsis thaliana
J. Exp. Bot.
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875-889
2008
Zea mays (Q5JZX2), Zea mays
brenda
Aranda, E.; Sampedro, I.; Diaz, R.; Garcia, M.; Ocampo, J.A.; Garcia-Romera, I.
Xyloglucanases in the interaction between saprobe fungi and the arbuscular mycorrhizal fungus Glomus mosseae
J. Plant Physiol.
164
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2007
Lactuca sativa, Pisum sativum
brenda
Liu, Y.B.; Lu, S.M.; Zhang, J.F.; Liu, S.; Lu, Y.T.
A xyloglucan endotransglucosylase/hydrolase involves in growth of primary root and alters the deposition of cellulose in Arabidopsis
Planta
226
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2007
Arabidopsis thaliana
brenda
Michailidis, G.; Argiriou, A.; Darzentas, N.; Tsaftaris, A.
Analysis of xyloglucan endotransglycosylase/hydrolase (XTH) genes from allotetraploid (Gossypium hirsutum) cotton and its diploid progenitors expressed during fiber elongation
J. Plant Physiol.
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Gossypium barbadense, Gossypium hirsutum (B2KL33), Gossypium hirsutum (B2KL34), Gossypium hirsutum (Q7Y252), Gossypium arboreum (B7SCZ6), Gossypium raimondii (B7SCZ7)
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Miedes, E.; Lorences, E.P.
Xyloglucan endotransglucosylase/hydrolases (XTHs) during tomato fruit growth and ripening
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Solanum lycopersicum (Q6RHX7), Solanum lycopersicum (Q6RHX8), Solanum lycopersicum (Q6RHX9), Solanum lycopersicum (Q6RHY0), Solanum lycopersicum (Q6RHY1), Solanum lycopersicum (Q9FR51), Solanum lycopersicum (Q9FZ05), Solanum lycopersicum (Q9SDX0), Solanum lycopersicum (Q9SLN9), Solanum lycopersicum
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Kurasawa, K.; Matsui, A.; Yokoyama, R.; Kuriyama, T.; Yoshizumi, T.; Matsui, M.; Suwabe, K.; Watanabe, M.; Nishitani, K.
The AtXTH28 gene, a xyloglucan endotransglucosylase/hydrolase, is involved in automatic self-pollination in Arabidopsis thaliana
Plant Cell Physiol.
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2009
Arabidopsis thaliana
brenda
Mark, P.; Baumann, M.J.; Ekloef, J.M.; Gullfot, F.; Michel, G.; Kallas, A.M.; Teeri, T.T.; Brumer, H.; Czjzek, M.
Analysis of nasturtium TmNXG1 complexes by crystallography and molecular dynamics provides detailed insight into substrate recognition by family GH16 xyloglucan endo-transglycosylases and endo-hydrolases
Proteins
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820-836
2008
Tropaeolum majus
brenda
Sinitsyna, O.A.; Fedorova, E.A.; Pravilnikov, A.G.; Rozhkova, A.M.; Skomarovsky, A.A.; Matys, V.Y.; Bubnova, T.M.; Okunev, O.N.; Vinetsky, Y.P.; Sinitsyn, A.P.
Isolation and properties of xyloglucanases of Penicillium sp
Biochemistry (Moscow)
75
41-49
2010
Talaromyces verruculosus, Penicillium canescens
brenda
Gullfot, F.; Ibatullin, F.; Sundqvist, G.; Davies, G.; Brumer, H.
Functional characterization of xyloglucan glycosynthases from GH7, GH12, and GH16 scaffolds
Biomacromolecules
10
1782-1788
2009
Bacillus licheniformis, Tropaeolum majus
brenda
Powlowski, J.; Mahajan, S.; Schapira, M.; Master, E.R.
Substrate recognition and hydrolysis by a fungal xyloglucan-specific family 12 hydrolase
Carbohydr. Res.
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Aspergillus niger
brenda
Kaida, R.; Kaku, T.; Baba, K.; Oyadomari, M.; Watanabe, T.; Nishida, K.; Kanaya, T.; Shani, Z.; Shoseyov, O.; Hayashi, T.
Loosening xyloglucan accelerates the enzymatic degradation of cellulose in wood
Mol. Plant
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904-909
2009
Populus sp.
brenda
Scarafoni, A.; Ronchi, A.; Duranti, M.
gamma-Conglutin, the Lupinus albus XEGIP-like protein, whose expression is elicited by chitosan, lacks of the typical inhibitory activity against GH12 endo-glucanases
Phytochemistry
71
142-148
2010
Aspergillus aculeatus
brenda
Ibatullin, F.M.; Banasiak, A.; Baumann, M.J.; Greffe, L.; Takahashi, J.; Mellerowicz, E.J.; Brumer, H.
A real-time fluorogenic assay for the visualization of glycoside hydrolase activity in planta
Plant Physiol.
151
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Arabidopsis thaliana, Tropaeolum majus
brenda
Gille, S.; Haensel, U.; Ziemann, M.; Pauly, M.
Identification of plant cell wall mutants by means of a forward chemical genetic approach using hydrolases
Proc. Natl. Acad. Sci. USA
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Arabidopsis thaliana
brenda
Wong, D.; Chan, V.; McCormack, A.; Batt, S.
A novel xyloglucan-specific endo-beta-1,4-glucanase: Biochemical properties and inhibition studies
Appl. Microbiol. Biotechnol.
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uncultured bacterium (D2K7Z0)
brenda
Ariza, A.; Ekloef, J.M.; Spadiut, O.; Offen, W.A.; Roberts, S.M.; Besenmatter, W.; Friis, E.P.; Skjot, M.; Wilson, K.S.; Brumer, H.; Davies, G.
Structure and activity of Paenibacillus polymyxa xyloglucanase from glycoside hydrolase family 44
J. Biol. Chem.
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33890-33900
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Paenibacillus polymyxa
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Menon, V.; Prakash, G.; Rao, M.
Enzymatic hydrolysis and ethanol production using xyloglucanase and Debaromyces hansenii from tamarind kernel powder: galactoxyloglucan predominant hemicellulose
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Thermomonospora sp.
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Maris, A.; Kaewthai, N.; Ekloef, J.M.; Miller, J.G.; Brumer, H.; Fry, S.C.; Verbelen, J.P.; Vissenberg, K.
Differences in enzymic properties of five recombinant xyloglucan endotransglucosylase/hydrolase (XTH) proteins of Arabidopsis thaliana
J. Exp. Bot.
62
261-271
2011
no activity in Arabidopsis thaliana
brenda
Harada, T.; Torii, Y.; Morita, S.; Onodera, R.; Hara, Y.; Yokoyama, R.; Nishitani, K.; Satoh, S.
Cloning, characterization, and expression of xyloglucan endotransglucosylase/hydrolase and expansin genes associated with petal growth and development during carnation flower opening
J. Exp. Bot.
62
815-823
2011
Dianthus caryophyllus (D7URZ0), Dianthus caryophyllus (D7URZ1), Dianthus caryophyllus (D7US89), Dianthus caryophyllus (Q1PCS6)
brenda
Choi, J.Y.; Seo, Y.S.; Kim, S.J.; Kim, W.T.; Shin, J.S.
Constitutive expression of CaXTH3, a hot pepper xyloglucan endotransglucosylase/hydrolase, enhanced tolerance to salt and drought stresses without phenotypic defects in tomato plants (Solanum lycopersicum cv. Dotaerang)
Plant Cell Rep.
30
867-877
2011
Capsicum annuum
brenda
Ichinose, H.; Araki, Y.; Michikawa, M.; Harazono, K.; Yaoi, K.; Karita, S.; Kaneko, S.
Characterization of an endo-processive-type xyloglucanase having a beta-1,4-glucan-binding module and an endo-type xyloglucanase from Streptomyces avermitilis
Appl. Environ. Microbiol.
78
7939-7945
2012
Streptomyces avermitilis (Q82K30), Streptomyces avermitilis (Q82M04), Streptomyces avermitilis, Streptomyces avermitilis NBRC 14893 (Q82M04), Streptomyces avermitilis DSM 46492 (Q82K30)
brenda
Feng, T.; Yan, K.P.; Mikkelsen, M.D.; Meyer, A.S.; Schols, H.A.; Westereng, B.; Mikkelsen, J.D.
Characterisation of a novel endo-xyloglucanase (XcXGHA) from Xanthomonas that accommodates a xylosyl-substituted glucose at subsite -1
Appl. Microbiol. Biotechnol.
98
9667-9679
2014
Xanthomonas citri (H8F9Y0), Xanthomonas citri LMG 941 (H8F9Y0)
brenda
Damasio, A.R.; Ribeiro, L.F.; Ribeiro, L.F.; Furtado, G.P.; Segato, F.; Almeida, F.B.; Crivellari, A.C.; Buckeridge, M.S.; Souza, T.A.; Murakami, M.T.; Ward, R.J.; Prade, R.A.; Polizeli, M.L.
Functional characterization and oligomerization of a recombinant xyloglucan-specific endo-beta-1,4-glucanase (GH12) from Aspergillus niveus
Biochim. Biophys. Acta
1824
461-467
2012
Aspergillus niveus, Aspergillus niveus A773
brenda
Matsuzawa, T.; Saito, Y.; Yaoi, K.
Key amino acid residues for the endo-processive activity of GH74 xyloglucanase
FEBS Lett.
588
1731-1738
2014
Paenibacillus sp. (Q3MUH7), Paenibacillus sp. KM21 (Q3MUH7)
brenda
Yoshizawa, T.; Shimizu, T.; Hirano, H.; Sato, M.; Hashimoto, H.
Structural basis for inhibition of xyloglucan-specific endo-beta-1,4-glucanase (XEG) by XEG-protein inhibitor
J. Biol. Chem.
287
18710-18716
2012
Daucus carota (Q05929)
brenda
Menon, V.; Rao, M.
Mechanistic insights into the inhibition of endo-beta 1,4 xyloglucan hydrolase by a classical aspartic protease inhibitor
J. Fluoresc.
23
311-321
2013
Thermomonospora sp.
brenda
Habrylo, O.; Forster, A.; Jeltsch, J.M.; Phalip, V.
The characterisation of xyloglucanase inhibitors from Humulus lupulus
Phytochemistry
90
70-77
2013
Aspergillus aculeatus
brenda
Kaewthai, N.; Gendre, D.; Ekloef, J.M.; Ibatullin, F.M.; Ezcurra, I.; Bhalerao, R.P.; Brumer, H.
Group III-A XTH genes of Arabidopsis encode predominant xyloglucan endohydrolases that are dispensable for normal growth
Plant Physiol.
161
440-454
2013
Arabidopsis thaliana (P93046), Arabidopsis thaliana (Q9SJL9), Arabidopsis thaliana Col-0 (P93046), Arabidopsis thaliana Col-0 (Q9SJL9)
brenda
van den Brink, J.; van Muiswinkel, G.C.; Theelen, B.; Hinz, S.W.; de Vries, R.P.
Efficient plant biomass degradation by thermophilic fungus Myceliophthora heterothallica
Appl. Environ. Microbiol.
79
1316-1324
2013
Aspergillus niger, Thermothelomyces heterothallicus, Trichoderma reesei, Thermothelomyces thermophilus, Thermothelomyces thermophilus ATCC 424674, Thermothelomyces heterothallicus CBS 202.75, Thermothelomyces heterothallicus CBS 663.74
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Berezina, O.V.; Herlet, J.; Rykov, S.V.; Kornberger, P.; Zavyalov, A.; Kozlov, D.; Sakhibgaraeva, L.; Krestyanova, I.; Schwarz, W.H.; Zverlov, V.V.; Liebl, W.; Yarotsky, S.V.
Thermostable multifunctional GH74 xyloglucanase from Myceliophthora thermophila high-level expression in Pichia pastoris and characterization of the recombinant protein
Appl. Microbiol. Biotechnol.
101
5653-5666
2017
Thermothelomyces thermophilus (G2QHR7), Thermothelomyces thermophilus, Thermothelomyces thermophilus ATCC 42464 (G2QHR7), Thermothelomyces thermophilus VKPM F-244 (G2QHR7)
brenda
Arnal, G.; Stogios, P.J.; Asohan, J.; Skarina, T.; Savchenko, A.; Brumer, H.
Structural enzymology reveals the molecular basis of substrate regiospecificity and processivity of an exemplar bacterial glycoside hydrolase family 74 endo-xyloglucanase
Biochem. J.
475
3963-3978
2018
Paenibacillus odorifer (A0A1R0YRH0), Paenibacillus odorifer (A0A1R0ZNW0), Paenibacillus odorifer, Paenibacillus odorifer DSM 15391 (A0A1R0YRH0), Paenibacillus odorifer DSM 15391 (A0A1R0ZNW0)
brenda
Kooij, P.W.; Pullens, J.W.; Boomsma, J.J.; Schiott, M.
Ant mediated redistribution of a xyloglucanase enzyme in fungus gardens of Acromyrmex echinatior
BMC Microbiol.
16
81
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Acromyrmex echinatior
brenda
Attia, M.; Stepper, J.; Davies, G.J.; Brumer, H.
Functional and structural characterization of a potent GH74 endo-xyloglucanase from the soil saprophyte Cellvibrio japonicus unravels the first step of xyloglucan degradation
FEBS J.
283
1701-1719
2016
Cellvibrio japonicus (B3PKK9), Cellvibrio japonicus, Cellvibrio japonicus Ueda107 (B3PKK9)
brenda
Han, Y.; Ban, Q.; Hou, Y.; Meng, K.; Suo, J.; Rao, J.
Isolation and characterization of two persimmon xyloglucan endotransglycosylase/hydrolase (XTH) genes that have divergent functions in cell wall modification and fruit postharvest softening
Front. Plant Sci.
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624
2016
Diospyros kaki (A0A067XR63), Diospyros kaki (A0A067XRK9)
brenda
Xian, L.; Wang, F.; Yin, X.; Feng, J.X.
Identification and characterization of an acidic and acid-stable endoxyloglucanase from Penicillium oxalicum
Int. J. Biol. Macromol.
86
512-518
2016
Penicillium oxalicum (A0A0A7RRF2), Penicillium oxalicum, Penicillium oxalicum HP7-1 (A0A0A7RRF2)
brenda
Shi, Y.; Zhu, X.; Miller, J.; Gregson, T.; Zheng, S.; Fry, S.
Distinct catalytic capacities of two aluminium-repressed Arabidopsis thaliana xyloglucan endotransglucosylase/hydrolases, XTH15 and XTH31, heterologously produced in Pichia
Phytochemistry
112
160-169
2015
no activity in Pichia pastoris, Arabidopsis thaliana (P93046), Arabidopsis thaliana (Q38911)
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brenda
Olsen, S.; Popper, Z.; Krause, K.
Two sides of the same coin xyloglucan endotransglucosylases/hydrolases in host infection by the parasitic plant Cuscuta
Plant Signal. Behav.
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e1145336
2016
Cuscuta reflexa, Solanum lycopersicum (Q40144)
brenda
Han, Y.; Zhu, Q.; Zhang, Z.; Meng, K.; Hou, Y.; Ban, Q.; Suo, J.; Rao, J.
Analysis of xyloglucan endotransglycosylase/hydrolase (XTH) genes and diverse roles of isoenzymes during persimmon fruit development and postharvest softening
PLoS ONE
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e0123668
2015
Diospyros kaki (A0A067XSJ2), Diospyros kaki (G5DAC8), Diospyros kaki (G5DAC9), Diospyros kaki
brenda
Damasio, A.R.; Rubio, M.V.; Goncalves, T.A.; Persinoti, G.F.; Segato, F.; Prade, R.A.; Contesini, F.J.; de Souza, A.P.; Buckeridge, M.S.; Squina, F.M.
Xyloglucan breakdown by endo-xyloglucanase family 74 from Aspergillus fumigatus
Appl. Microbiol. Biotechnol.
101
2893-2903
2017
Aspergillus fumigatus (Q4WB93), Aspergillus fumigatus, Aspergillus fumigatus CBS 101355 (Q4WB93), Aspergillus fumigatus ATCC MYA-4609 (Q4WB93), Aspergillus fumigatus FGSC A1100 (Q4WB93), Aspergillus fumigatus Af293 (Q4WB93)
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