Information on EC 3.2.1.151 - xyloglucan-specific endo-beta-1,4-glucanase

Word Map on EC 3.2.1.151
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.151
-
RECOMMENDED NAME
GeneOntology No.
xyloglucan-specific endo-beta-1,4-glucanase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
xyloglucan + H2O = xyloglucan oligosaccharides
show the reaction diagram
endohydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
hydrolysis of O-glycosyl bond
-
-
-
-
transglycosylation
SYSTEMATIC NAME
IUBMB Comments
[(1->6)-alpha-D-xylo]-(1->4)-beta-D-glucan glucanohydrolase
The enzyme from Aspergillus aculeatus is specific for xyloglucan and does not hydrolyse other cell-wall components. The reaction involves endohydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan with retention of the beta-configuration of the glycosyl residues.
CAS REGISTRY NUMBER
COMMENTARY hide
76901-10-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
onion
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene xegA
-
-
Manually annotated by BRENDA team
gene xegA
-
-
Manually annotated by BRENDA team
-
QO5929
UniProt
Manually annotated by BRENDA team
soybean
-
-
Manually annotated by BRENDA team
289 amino acids, GaXTH1, similar to Gossypium hirsutum XTH1-2 gene sequence; var. 79/BH47, diploploid progenitor of Gossypium hirsutum, tree cotton
UniProt
Manually annotated by BRENDA team
Giza7 (long fibers, 1.8fold higher XTH expression than in Gossypium hirsutum Acala SJ2), sea-island cotton/Egyptian cotton
-
-
Manually annotated by BRENDA team
289 amino acids, GrXTH1, similar to Gossypium hirsutum gene sequence GhXTH1-1; var. Ulbrich, diploploid progenitor of Gossypium hirsutum, New World cotton
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in Arabidopsis thaliana
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene xegA encodes isozyme XG A
-
-
Manually annotated by BRENDA team
isoform XGH74A
-
-
Manually annotated by BRENDA team
recombinant enzyme
-
-
Manually annotated by BRENDA team
gene sav_2574
UniProt
Manually annotated by BRENDA team
gene sav_1856
UniProt
Manually annotated by BRENDA team
L. var. Climbing Giants, nasturtium
-
-
Manually annotated by BRENDA team
Thermomonospora sp.
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
from cow's rumen
UniProt
Manually annotated by BRENDA team
pv. mangiferaeindicae
UniProt
Manually annotated by BRENDA team
pv. mangiferaeindicae
UniProt
Manually annotated by BRENDA team
induction by gibberellins
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + Glcbeta(1->4)Glcbeta(1->4)Glcbeta
show the reaction diagram
-
3.8% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
-
?
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
show the reaction diagram
-
100% activity
-
-
?
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O
?
show the reaction diagram
-
-
-
-
?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + ?
show the reaction diagram
-
1.3% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
-
?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
?
show the reaction diagram
-
-
-
-
?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + ?
show the reaction diagram
-
19.4% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
-
?
arabinoxyloglucan + H2O
arabinoxyloglucan oligosaccharides
show the reaction diagram
-
-
-
-
?
azo-cellulose + H2O
azo-cellulose oligosaccharides
show the reaction diagram
-
-
-
?
barley beta-glucan + H2O
?
show the reaction diagram
-
87% activity compared to tamarind xyloglucan
-
-
?
beta-glucan + H2O
beta-glucan oligosaccharides
show the reaction diagram
carboxyethyl cellulose + H2O
carboxyethyl cellulose oligosaccharides
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
?
carboxymethyl cellulose + H2O
carboxymethyl cellulose oligosaccharides
show the reaction diagram
carboxymethyl curdlan + H2O
carboxymethyl curdlan oligosaccharides
show the reaction diagram
-
less than 2% of the activity with xyloglucan
-
?
carboxymethyl pachyman + H2O
carboxymethyl pachyman oligosaccharides
show the reaction diagram
-
less than 2% of the activity with xyloglucan
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
high activity
-
-
?
carob galactomannan + H2O
?
show the reaction diagram
-
4% activity compared to tamarind xyloglucan
-
-
?
cellohexaose + H2O
?
show the reaction diagram
-
-
-
-
?
cellopentaose + H2O
?
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
?
show the reaction diagram
-
-
-
-
?
fucoxyloglucan + H2O
fucoxyloglucan oligosaccharides
show the reaction diagram
-
-
-
-
?
galactoxyloglucan + H2O
?
show the reaction diagram
Thermomonospora sp.
-
-
-
-
?
GGXXXG + H2O
Glc(beta1-4)Glc + XXXG
show the reaction diagram
-
-
-
-
?
Glcbeta(1-3)Glcbeta(1-3)Glc + H2O
D-glucose + Glcbeta(1-3)Glc
show the reaction diagram
-
-
38% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glc(1-4)Glc + H2O
D-glucose + Glcbeta(1-4)Glc
show the reaction diagram
-
-
32% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glc(1-4)Glcbeta(1-4)Glc + H2O
Glcbeta(1-4)Glc
show the reaction diagram
-
-
76% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glc-2-chloro-4-nitrophenol + H2O
D-glucose + 2-chloro-4-nitrophenyl-beta-D-glucoside
show the reaction diagram
-
24% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
-
?
Glcbeta(1-4)Glcbeta(1-3)Glc + H2O
D-glucose + Glcbeta(1-3)Glc
show the reaction diagram
-
-
52% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glcbeta(1-4)Glc(1-4)Glc-2-chloro-4-nitrophenol + H2O
Glcbeta(1-4)Glc(1-4)Glc + 2-chloro-4-nitrophenyl-beta-D-glucoside
show the reaction diagram
-
-
84% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glcbeta(1-4)Glc-2-chloro-4-nitrophenol + H2O
Glcbeta(1-4)Glc + 2-chloro-4-nitrophenyl-beta-D-glucoside
show the reaction diagram
-
-
68% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-3)Glc + H2O
Glcbeta(1-4)Glc + Glcbeta(1-3)Glc
show the reaction diagram
-
-
83% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc + H2O
Glcbeta(1-4)Glcbeta(1-4)Glc + GG
show the reaction diagram
-
-
-
-
?
Glcbeta(1->4)[Xylalpha(1->6)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
?
show the reaction diagram
-
-
-
-
?
GXGXXXG + H2O
GXG + XXXG
show the reaction diagram
-
-
-
-
?
hydroxyethyl cellulose + H2O
?
show the reaction diagram
-
65% activity compared to tamarind xyloglucan
-
-
?
hydroxyethyl cellulose + H2O
hydroxyethyl cellulose oligosaccharides
show the reaction diagram
-
-
-
-
?
laminarin + H2O
laminarin oligosaccharides
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
?
lichenan + H2O
lichenan cellulose oligosaccharides
show the reaction diagram
-
-
-
-
?
octodecasaccharide XLLG-XLLG + H2O
?
show the reaction diagram
-
xylogluco-nonasaccharide
-
-
?
phosphoric acid-swollen cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
sulforhodamine-XLLG + H2O
?
show the reaction diagram
tamarind seed xyloglucan + H2O
?
show the reaction diagram
QO5929
-
-
-
?
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc
show the reaction diagram
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc (1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-alpha-D-Xyl-(1->6)-beta-D-Glc-L-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc
show the reaction diagram
tamarind xyloglucan + H2O
?
show the reaction diagram
wheat arabinoxylan + H2O
?
show the reaction diagram
-
15% activity compared to tamarind xyloglucan
-
-
?
XGXXXG + H2O
XG + XXXG
show the reaction diagram
-
-
-
-
?
XXGXXXG + H2O
XXG + XXXG
show the reaction diagram
-
-
-
-
?
XXXG + H2O
XX + XG
show the reaction diagram
-
X stands for a substituted glucose residue, G for an unsubstituted glucose residue
270% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
XXXGXX + H2O
XXXG + XX
show the reaction diagram
-
-
-
-
?
XXXGXXX + H2O
XXXG + XX
show the reaction diagram
-
-
-
-
?
XXXGXXXG + H2O
XX + XGXX + XG
show the reaction diagram
-
X stands for a substituted glucose residue, G for an unsubstituted glucose residue
hydrolysis at both chain ends of substrate. 390% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
XXXGXXXG + H2O
XXX + XXXG + GXXXG
show the reaction diagram
XXXGXXXG + H2O
XXXG
show the reaction diagram
XXXXGXXXXG + H2O
XXXXG
show the reaction diagram
-
-
-
-
?
Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta + H2O
?
show the reaction diagram
-
-
-
-
?
xyloglucan + H2O
?
show the reaction diagram
xyloglucan + H2O
xyloglucan oligosaccharides
show the reaction diagram
xyloglucan heptasaccharide + H2O
?
show the reaction diagram
tritium labeled
-
-
?
xyloglucan oligosaccharide + H2O
?
show the reaction diagram
-
diverse substrates, substrate specificity, overview
-
-
?
xyloglucan oligosaccharide HDP-XGO + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
xyloglucan + H2O
?
show the reaction diagram
-
xyloglucan endotransglucosylase/hydrolases are a family of enzymes that mediate the construction and restructuring of xyloglucan cross-links, thereby controlling the extensibility or mechanical properties of the cell wall in a wide variety of plant tissues
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
show the reaction diagram
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta-1,4-cellobiosyloxazine
-
competitive inhibitor
-
beta-1,4-glucosyl-noeuromycin
-
competitive inhibitor
-
extracellular dermal glycoprotein
QO5929
a glycoside hydrolase inhibitor protein inhibitor of the enzyme, it closely mimics the XEG-xyloglucan interaction
-
Humulus lupulus xyloglucan-specific endoglucanase inhibitor proteins
-
XEGIPs, the inhibitor proteins act specifically against the members of fungal glycoside hydrolase family 12, three homologues: HlXEGIP1 causes 85% inhibition at 0.03 mg, HlXEGIP2 95%, but HlXEGIP3 is inactive. Physiological function and enzyme interaction of the xyloglucan-specific endoglucanase inhibitor proteins, cloning and phylogenetic as well as expression analysis in hop, overview
-
pepstatin A
Thermomonospora sp.
-
a specific inhibitor towards aspartic proteases, inactivates the enzyme, reversible, competitive, two-step inhibition mechanism
XXXG
the addition of XXXG results in linear noncompetitive inhibition on the hydrolysis of tamarind xyloglucan
xyloglucan-specific endo-beta-1,4-glucanase inhibitor proteins
-
XEGIPs, e.g. from tomato, carrot, or tobacco, inhibhition by formation of tight complexes
-
additional information
-
no inhibition by gamma-conglutin from Lupinus albus, although it exhibits high structural similarity to xyloglucan-specific endo-beta-1,4-glucanase inhibitor proteins, overview
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
epibrassinolide
induction in etiolated seedling
gibberellic acid
-
induction
gibberellin a3
induction in etiolated seedling
iodoacetic acid
induction in green seedling; induction in green seedling; induction in green seedling
oligosaccharides
-
xyloglucan oligosaccharides cause cell wall loosening by enhancing xyloglucan endotransglucosylase/hydrolase activity in azuki bean epicotyls
-
additional information
Thermomonospora sp.
-
the exogenous addition of beta-galactosidase to xyloglucanase improves the overall hydrolysis of galactoxyloglucan by 30%. The rate of hydrolysis of galactoxyloglucan with xyloglucanase is increased by 15-20% in the presence of Tween 80 and toluene or bovine serum albumin
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.361
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
apparent value, at pH 6.0 and 30C
0.029
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
apparent value, at pH 6.0 and 30C
0.12
XXXG
-
37C, pH 5
0.134
Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta
-
apparent value, at pH 6.0 and 30C
0.998
Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta
-
apparent value, at pH 6.0 and 30C
0.08 - 3.6
xyloglucan
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.34
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta
Paenibacillus polymyxa
-
apparent value, at pH 6.0 and 30C
15.93
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
Paenibacillus polymyxa
-
apparent value, at pH 6.0 and 30C
306
beta-Glucan
Trichoderma reesei
-
37C, pH 5
0.22
carboxyethyl cellulose
Ruminiclostridium thermocellum
-
pH 7.0, 50C
-
0.72 - 52
carboxymethyl cellulose
-
138
hydroxyethyl cellulose
Trichoderma reesei
-
37C, pH 5
193
Laminarin
Trichoderma reesei
-
37C, pH 5
0.59
lichenan
Ruminiclostridium thermocellum
-
pH 7.0, 50C
7
phosphoric acid-swollen cellulose
Trichoderma reesei
-
37C, pH 5
-
49.2 - 72
tamarind seed xyloglucan
-
1.24
tamarind xyloglucan
uncultured bacterium
D2K7Z0
at 37C, pH 7.0
-
7.2
XXXG
Trichoderma reesei
-
37C, pH 5
14.01
Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta
Paenibacillus polymyxa
-
apparent value, at pH 6.0 and 30C
6.96
Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta
Paenibacillus polymyxa
-
apparent value, at pH 6.0 and 30C
0.012 - 805
xyloglucan
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20.33
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta
Paenibacillus polymyxa
-
apparent value, at pH 6.0 and 30C
41687
541.7
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
Paenibacillus polymyxa
-
apparent value, at pH 6.0 and 30C
28474
104.7
Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta
Paenibacillus polymyxa
-
apparent value, at pH 6.0 and 30C
41686
6.98
Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta
Paenibacillus polymyxa
-
apparent value, at pH 6.0 and 30C
28473
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
125
pepstatin A
Thermomonospora sp.
-
pH 7.0, 50C
1.46
XXXG
pH and temperature not specified in the publication
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0035
pepstatin A
Thermomonospora sp.
-
pH 7.0, 50C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.01
-
substrate: xyloglucan, pH 5.0, 70C
0.02
-
substrate: xyloglucan, pH 5.0, 50C
0.1
-
substrate: xyloglucan, pH 5.0, 50C
0.2
-
substrate: xyloglucan, pH 5.0, 70C
0.5
-
substrate: xyloglucan, pH 5.0, 50C
0.7
-
substrate: xyloglucan, pH 5.0, 70C
0.75
-
substrate: xyloglucan, pH 5.0, 70C
1.294
-
-
1.6
-
substrate: xyloglucan, pH 5.0, 50C
3 - 8
-
purified XG 70, substrate is tamarind xyloglucan
19
substrate: xyloglucan
21
-
purified XG A, substrate is tamarind xyloglucan
52
-
purified XG 25, substrate is tamarind xyloglucan
113
-
substrate pea seed xyloglucan
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6
QO5929
assay at
4.8
-
both isoforms NXG1 and NXG2
additional information
-
enzyme initially produces oligosaccharides with a degree of polymerization of 16-18, which are slowly hydrolyzed to final products with a degree of polymerization of 7-9. The ratio of the oligosaccharides with 7-9 to 16-18 glucose units is additionally dependent upon the pH value
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 9.5
activity range, profile overview
3.8 - 6.1
-
more than 50% of maximal activity in the pH-range 3.8-6.1
4 - 7.5
-
activity range
4 - 7
activity range, profile overview
4.5 - 8
-
active between pH 4.5 and pH 8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
QO5929
assay at room temperature
37 - 50
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 80
-
activity range
30 - 60
activity range, rapid loss of activity above 60C, profile overview
30 - 75
activity range, profile overview
50 - 70
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.8
-
isoelectrofocusing
3.4
pH gradient 3.5-9.5
4.38
-
sequence calculation
4.5
calculated from amino acid sequence
4.8
-
chromatofocusing
4.96
-
XG A, sequence calculation
8
-
PI over 8
8.1
-
pH gradient 6-9
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
systemical accumulation of xyloglucan endotransglycosylase/hydrolase transcripts following infestation with Myzus persicae. Induction is specific to phloem but not correlated with increase in enzyme activity in phloem
Manually annotated by BRENDA team
-
germinating
Manually annotated by BRENDA team
shoot of etiolated seedling; shoot of etiolated seedling
Manually annotated by BRENDA team
-
several abundant XTH genes are generally up-regulated during tension formation, including XTH16-36, -21, -14 and -27, while others were suppressed such as XTH16-35. In mature gelatinous fibers, enzyme gene products are detected in the gelatinous layers while the enzyme's endo-transglycosylase activity is found in the adjacent S2 wall layer. Enzyme gene products and activity persist in mature gelatinous fibers for several years
Manually annotated by BRENDA team
additional information
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / NCTC 11153)
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372)
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372)
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372)
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372)
Geotrichum sp. (strain M128)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
13000
-
gel filtration
23300
x * 23300, calculated, mature protein, x * 32000, SDS-PAGE
23500
-
x * 23500, about, sequence calculation, the enzyme is a predominantly a monomer at 60C and is more active in its monomeric state
23600
mass spectrometry
26154
-
x * 25000, XG A, SDS-PAGE, x * 26154, XG A, sequence calculation
31000
x * 31000, calculated; x * 31000, calculated; x * 31000, calculated
31800
x * 31800, calculated
32400
x * 32400, calculated
33000
-
SDS-PAGE
40000
x * 40000, SDS-PAGE
45455
x * 45455, calculated from amino acid sequence
77000
-
SDS-PAGE
78000
x * 78000, recombinant enzyme, SDS.PAGE
80500
-
x * 80500, MALDI-TOF
90500
calculated from amino acid sequence
92000
x * 92000, recombinant enzyme, SDS-PAGE
105000
x * 105000, SDS-PAGE
130000
-
x * 80000, SDS-PAGE, enzyme without C-terminal carbohydratebinding module domain, x * 130000, SDS-PAGE, enzyme with C-terminal carbohydratebinding module domain
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer or dimer
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
possibly both N- and O-glycosylation
no glycoprotein
N-glycosylation site at N32 is not glycosylated
proteolytic modification
sequence contains a signal peptide of 15 amino acids
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
unliganded and in complex with xyloglucan oligosaccharide. Enzyme displays an open active center groove grafted upon its beta-jelly roll fold, in which the side chaindecorations of xyloglucan may be accomodated. Binding of xylosyl and pendant galactosyl moieties is tolerated, but the enzyme is similarly competent in the binding of unbranched glucans
-
purified enzyme free or in complex with substrate xyloglucan or inhibitor extracellular dermal glycoprotein, hanging drop vapor diffusion method, from 0.1 M sodium acetate, pH 5.5, and 1.5 M ammonium sulfate, or 0.1 M sodium acetate, pH 4.6, 25% PEG 3000, and 5 mg/ml digested xyloglucan, 20C, X-ray diffraction structure determination and analysis at 0.95-2.70 A resolution
QO5929
unliganded and in complex with xyloglucan oligosaccharide. Enzyme displays an open active center groove grafted upon its (beta/alpha)8 fold, in which the side chaindecorations of xyloglucan may be accomodated. Kinetically productive interactions are made with both xylose and galactose substituents
-
hanging drop vapor diffusion method, using 0.1 M bis-Tris, pH 6.5, 0.2 M Li2SO4, 25% (w/v) PEG 3350
-
unliganded wild-type and mutant D70A in complex with oligosaccharide substrate
-
isoforms NXG1 and NXG2, comparison with data from the strict endo-transglycolase from Populus tremula x Populus tremuloides
-
loop mutant TmNXG1-DELTAYNIIG with an oligosaccharide product bound in the negative active-site subsites, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement, molecular dynamics simulations and structure modelling of wild-type and mutant enzyme, detailed overview; TmNXG1-deltaYNIIG with bound substrate xylogluco-oligosaccharide
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 3.8
optimally stable, stability declines sharply below pH 2.8 and above pH 5
646677
4 - 9
purified enzyme, stable at
731816
4.5 - 9
purified recombinant enzyme, stable at
731175
5
-
40C, stable for at least 24 h
658199
5 - 7.5
stable
667262
5 - 8
at 45C, stable
667262
5
the enzyme is relatively stable at pH above 5.0, where the enzyme retains more than 95% of the activity
713867
5.5 - 6.5
-
45C, stable
668589
5.5 - 8.5
-
25C, stable
668589
6 - 6.5
purified recombinant enzyme, stable at
731175
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 50
purified recombinant enzyme, stable at, rapid loss of activity above 50C, profile overview
50 - 70
the enzyme is stable up to 50C. The enzyme is completely inactivated by incubation at 70C for 10 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
concentration with Centricon devices, cutoff 10 kDa
-
endoI, endoIV and endoV
-
homogeneity
native extracellular enzyme from culture filtrate by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
Thermomonospora sp.
-
native extracellular enzyme from culture medium by anion exchange chromatography
-
native extracellular enzyme from culture medium by anion exchange chromatography to over 95% purity
-
recombinant GST-tagged enzyme from Escherichia coli BL21 by glutathione affinity chromatography and gel filtration
QO5929
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlus (DE3) RP by nickel affinity chromatography and gel filtration
recombinant; recombinant
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Agrobacterium tumefaciens and SYN947-D binary vector for transfection of AtXTH28 fragment into atxth28 null-mutant
-
by gel filtration
-
DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression in Escherichia coli
expressed in Escherichia coli BL21 (DE3)pLysE cells
expressed in Solanum lycopersicum cultivar Dotaerang
-
expression in Arabidopsis thaliana
expression in Aspergillus niger
expression in Escherichia coli
expression in Escherichia coli and Pichia pastoris
expression in Escherichia coli; expression in Escherichia coli
expression in Pichia pastoris
-
expression in Pichia pastoris, with and without the C-terminal carbohydratebinding module domain
-
expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-CodonPlus (DE3) RP
expression of GST-tagged enzyme in Escherichia coli BL21
QO5929
gene sav_1856, DNA and amino acid sequence determination and analysis; gene sav_2574, DNA and amino acid sequence determination and analysis
gene xegA, DNA and amino acid sequence determination and analysis
-
gene XTH31, phylogenetic analysis, Arabidopsis thaliana contains 33 phylogenetically diverse xyloglucan endo-transglycosylase/hydrolase (XTH) gene products, two of which, genes AtXTH31 and AtXTH32, are predominant xyloglucan endohydrolases due to clustering into group III-A, DNA and amino acid sequence determination and analysis; gene XTH32, phylogenetic analysis, Arabidopsis thaliana contains 33 phylogenetically diverse xyloglucan endo-transglycosylase/hydrolase (XTH) gene products, two of which, genes AtXTH31 and AtXTH32, are predominant xyloglucan endohydrolases due to clustering into group III-A, DNA and amino acid sequence determination and analysis
heterologous expression in Pichia pastoris strain GS115
-
overexpression of the enzyme in poplar trees
-
XEG113, DNA and amino acid sequence determination and analysis, genotyping, overview
-
XTH-DNA from leaves in pCR 2.1-TOPO vector
XTH-DNA from leaves in pCR 2.1-TOPO vector; XTH-DNA from leaves in pCR 2.1-TOPO vector; XTH-DNA from leaves in pCR 2.1-TOPO vector, rolling circle amplification RACE
XTH-DNA from leaves in pCR 2.1-TOPO vector; XTH-DNA from leaves in pCR 2.1-TOPO vector; XTH-DNA from leaves in pCR 2.1-TOPO vector; XTH-DNA from leaves in pCR 2.1-TOPO vector; XTH-DNA from leaves in pCR 2.1-TOPO vector; XTH-DNA from leaves in pCR 2.1-TOPO vector; XTH-DNA from leaves in pCR 2.1-TOPO vector; XTH-DNA from leaves in pCR 2.1-TOPO vector; XTH-DNA from leaves in pCR 2.1-TOPO vector
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E155A
-
the mutant acts as glucosynthase and can perform the condensation of xyloglucosyl fluorides, albeit at poor rates
W13A
QO5929
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
W13A/W28A
QO5929
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
W28A
QO5929
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
Y24A
QO5929
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
E358S
-
nucleophile mutant
K129A/R156Y
-
increased stability mutant
W318A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
W319A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
W61A
site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme
W64A
site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme
W318A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
-
W319A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
-
W61A
-
site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme
-
W64A
-
site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme
-
D74A
-
crystallization data in complex with oligosaccharide substrate
DELTAYNIIG
-
loop deletion variant of isoform NXG1, structurally similar to the strict endo-transglycolase from Populus tremula x Populus tremuloides. Mutant has a greatly increased transglycosylation:hydrolysis ratio
E94A
-
the GH16 xyloglucan hydrolase mutant of TmNXG1 acting as glycosynthase is capable of synthesizing XLLG-based xyloglucan oligosaccharides at rates feasible for preparative synthesis, thus providing an essential expansion of product ranges
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
degradation
-
xyloglucan endotransglucosylase can act as a cell wall-loosening enzyme
synthesis