3.2.1.151: xyloglucan-specific endo-beta-1,4-glucanase
This is an abbreviated version!
For detailed information about xyloglucan-specific endo-beta-1,4-glucanase, go to the full flat file.
Word Map on EC 3.2.1.151
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3.2.1.151
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xyloglucans
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tamarind
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microfibrils
-
xxxg
-
expansins
-
wall-modifying
-
hemicellulosic
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2.4.1.207
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mixed-linkage
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tropaeolum
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nasturtium
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agriculture
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synthesis
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myceliophthora
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degradation
- 3.2.1.151
- xyloglucans
- tamarind
-
microfibrils
- xxxg
-
expansins
-
wall-modifying
-
hemicellulosic
-
2.4.1.207
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mixed-linkage
-
tropaeolum
- nasturtium
- agriculture
- synthesis
-
myceliophthora
- degradation
Reaction
Synonyms
AfXEG74, AspGD, AtXTH31, AtXTH32, bacterial glycoside hydrolase family 74 endo-xyloglucanase, BlXG12, CaXTH1, Cel74A, CJA_2477, CjGH74, DkXTH3, DkXTH4, DkXTH5, DkXTH6, DkXTH7, endo-1,4-beta-glucanase/xyloglucanase, endo-beta 1,4 xyloglucan hydrolase, endo-beta-1,4-(xylo)glucan hydrolase, endo-beta-1,4-xyloglucanase, endo-processive xyloglucanase, endo-xyloglucanase, endoxyloglucanase, family GH16 xyloglucan endo-transglycosylase, GH12 xyloglucanase, GH16 xyloglucan hydrolase, GH74 endo-xyloglucanase, GH74 xyloglucanase, Gly74A, GT77, LeXTH1, More, Mt-XTH1, MtXgh74, MYCTH_116384, NXG1, oligoxyloglucan hydrolase, PODO_RS11130, PoGH74, POXEG12A, PoxXEG12A, SaGH74A, SaGH74B, SIXTH5, TmNXG1, TXy, XcXGHA, XEG, Xeg1, XEG12A, XEG5, XEG5A, XEG74, XegA, XEH, XET, XG 25, XG 70, XG A, XG-ase, XG12, XG44, XG5, XGH74, Xgl74A, XH, XTH, XTH1, XTH15, XTH2, XTH3, XTH31, XTH4, XTH6, XTH7, xyloglucan endo-beta-1,4-glucanase, xyloglucan endohydrolase, xyloglucan endotransglucosylase/hydrolase, xyloglucan endotransglucosylase/hydrolase 3, xyloglucan endotransglucosylase/hydrolase protein 31, xyloglucan endotransglucosylase/hydrolase protein 32, xyloglucan endotransglucosylase/hydrolases, xyloglucan endotransglycosylase/hydrolase, xyloglucan hydrolase, xyloglucan-specific beta-(1,4)-glucanase, xyloglucan-specific endo 1,4-beta-glucanase, xyloglucan-specific endo-1,4beta-glucanase, xyloglucan-specific endo-beta-1,4-glucanase, xyloglucan-specific family 12 hydrolase, xyloglucanase, xyloglucanase 1, xyloglucanase A, xyloglucanendohydrolase, xyloglycan hydrolase
ECTree
3.2.1.151 xyloglucan-specific endo-beta-1,4-glucanaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.2.1.151 - xyloglucan-specific endo-beta-1,4-glucanase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
3.8% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
-
?
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
100% activity
-
-
?
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O
?
-
-
-
-
?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + ?
-
1.3% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
-
?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
?
-
-
-
-
?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + ?
-
19.4% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
carboxyethyl cellulose + H2O
carboxyethyl cellulose oligosaccharides
-
-
-
-
?
carboxymethyl curdlan + H2O
carboxymethyl curdlan oligosaccharides
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less than 2% of the activity with xyloglucan
-
?
carboxymethyl pachyman + H2O
carboxymethyl pachyman oligosaccharides
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less than 2% of the activity with xyloglucan
-
?
carob galactomannan + H2O
?
-
4% activity compared to tamarind xyloglucan
-
-
?
Glcbeta(1-3)Glcbeta(1-3)Glc + H2O
D-glucose + Glcbeta(1-3)Glc
-
-
38% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glc(1-4)Glc + H2O
D-glucose + Glcbeta(1-4)Glc
-
-
32% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glc(1-4)Glcbeta(1-4)Glc + H2O
Glcbeta(1-4)Glc
-
-
76% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glc-2-chloro-4-nitrophenol + H2O
D-glucose + 2-chloro-4-nitrophenyl-beta-D-glucoside
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24% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
-
?
Glcbeta(1-4)Glcbeta(1-3)Glc + H2O
D-glucose + Glcbeta(1-3)Glc
-
-
52% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glcbeta(1-4)Glc(1-4)Glc-2-chloro-4-nitrophenol + H2O
Glcbeta(1-4)Glc(1-4)Glc + 2-chloro-4-nitrophenyl-beta-D-glucoside
-
-
84% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glcbeta(1-4)Glc-2-chloro-4-nitrophenol + H2O
Glcbeta(1-4)Glc + 2-chloro-4-nitrophenyl-beta-D-glucoside
-
-
68% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-3)Glc + H2O
Glcbeta(1-4)Glc + Glcbeta(1-3)Glc
-
-
83% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc + H2O
Glcbeta(1-4)Glcbeta(1-4)Glc + GG
-
-
-
-
?
Glcbeta(1->4)[Xylalpha(1->6)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
?
-
-
-
-
?
hydroxyethyl cellulose + H2O
hydroxyethyl cellulose oligosaccharides
-
-
-
-
?
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc (1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-alpha-D-Xyl-(1->6)-beta-D-Glc-L-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc
tamarind xyloglucan + H2O
tamarind xyloglucooligosaccharides
XTH31 cleaves the substrate from about 186 kDa to about 6 kDa (median sizes) within 40 h. The enzyme does not have a preferred cleavage site close to either terminus
-
-
?
wheat arabinoxylan + H2O
?
-
15% activity compared to tamarind xyloglucan
-
-
?
XXXG + H2O
XX + XG
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X stands for a substituted glucose residue, G for an unsubstituted glucose residue
270% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
XXXGXXXG + H2O
XX + XGXX + XG
-
X stands for a substituted glucose residue, G for an unsubstituted glucose residue
hydrolysis at both chain ends of substrate. 390% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
-
?
Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta + H2O
?
-
-
-
-
?
Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
?
-
-
-
-
?
xyloglucan heptasaccharide + H2O
?
tritium labeled
-
-
?
xyloglucan oligosaccharide + H2O
?
-
diverse substrates, substrate specificity, overview
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
Thermothelomyces thermophilus VKPM F-244
-
-
-
?
barley beta-glucan + H2O
?
-
lower activity compared to xyloglucan
-
-
?
barley beta-glucan + H2O
?
-
87% activity compared to tamarind xyloglucan
-
-
?
barley beta-glucan + H2O
?
4% activity compared to tamarind seed xyloglucan
-
-
?
barley beta-glucan + H2O
?
4% activity compared to tamarind seed xyloglucan
-
-
?
barley beta-glucan + H2O
?
Thermothelomyces thermophilus VKPM F-244
4% activity compared to tamarind seed xyloglucan
-
-
?
barley-beta-glucan + H2O
?
approximately 50fold lower specific activity for the natural mixed-linkage (1->3)/(1->4)-beta-glucan from barley compared to xyloglucan
-
-
?
barley-beta-glucan + H2O
?
approximately 50fold lower specific activity for the natural mixed-linkage (1->3)/(1->4)-beta-glucan from barley compared to xyloglucan
-
-
?
carboxymethyl cellulose + H2O
?
enzyme CjGH74 shows approximately 165fold lower specific activity for the artificial polysaccharide derivative hydroxyethyl cellulose compared to xyloglucan
-
-
?
carboxymethyl cellulose + H2O
?
enzyme CjGH74 shows approximately 165fold lower specific activity for the artificial polysaccharide derivative hydroxyethyl cellulose compared to xyloglucan
-
-
?
carboxymethyl cellulose + H2O
carboxymethyl cellulose oligosaccharides
-
carboxymethyl cellulose-4M
-
-
?
carboxymethyl cellulose + H2O
carboxymethyl cellulose oligosaccharides
-
-
-
-
?
carboxymethyl cellulose + H2O
carboxymethyl cellulose oligosaccharides
-
-
-
-
?
carboxymethylcellulose + H2O
?
2% activity compared to tamarind seed xyloglucan
-
-
?
carboxymethylcellulose + H2O
?
2% activity compared to tamarind seed xyloglucan
-
-
?
carboxymethylcellulose + H2O
?
Thermothelomyces thermophilus VKPM F-244
2% activity compared to tamarind seed xyloglucan
-
-
?
hydroxyethyl cellulose + H2O
?
enzyme CjGH74 shows approximately 24fold lower specific activity for the artificial polysaccharide derivative hydroxyethyl cellulose compared to xyloglucan
-
-
?
hydroxyethyl cellulose + H2O
?
enzyme CjGH74 shows approximately 24fold lower specific activity for the artificial polysaccharide derivative hydroxyethyl cellulose compared to xyloglucan
-
-
?
hydroxyethyl cellulose + H2O
?
-
65% activity compared to tamarind xyloglucan
-
-
?
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc
-
-
-
?
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc
-
-
-
?
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc (1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-alpha-D-Xyl-(1->6)-beta-D-Glc-L-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc
-
-
-
?
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc (1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-alpha-D-Xyl-(1->6)-beta-D-Glc-L-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc
Xanthomonas citri LMG 941
-
-
-
?
tamarind seed xyloglucan + H2O
xyloglucooligosaccharides
enzyme CjGH74 acting on tamarind XyG reveals that the catalytic module hydrolyzes the polysaccharide at unbranched backbone glucosyl residues to generate the oligosaccharides XXXG, XLXG, XXLG and XLLG, which differ in their degree of side-chain galactosylation. This is the most common cleavage pattern observed for GH74 endo-xyloglucanases
-
-
?
tamarind seed xyloglucan + H2O
xyloglucooligosaccharides
the enzyme specifically hydrolyzes tamarind xyloglucan in endo-acting mode
-
-
?
tamarind seed xyloglucan + H2O
xyloglucooligosaccharides
Penicillium oxalicum HP7-1
the enzyme specifically hydrolyzes tamarind xyloglucan in endo-acting mode
-
-
?
tamarind seed xyloglucan + H2O
xylooligosaccharides
best substrate, enzyme MtXgh74 hydrolyzes various linkages within the xyloglucan building blocks XXXG, XXLG, and XLXG (except XLLG) producing diverse low molecular weight oligosaccharides
-
-
?
tamarind seed xyloglucan + H2O
xylooligosaccharides
best substrate, enzyme MtXgh74 hydrolyzes various linkages within the xyloglucan building blocks XXXG, XXLG, and XLXG (except XLLG) producing diverse low molecular weight oligosaccharides
-
-
?
tamarind seed xyloglucan + H2O
xylooligosaccharides
Thermothelomyces thermophilus VKPM F-244
best substrate, enzyme MtXgh74 hydrolyzes various linkages within the xyloglucan building blocks XXXG, XXLG, and XLXG (except XLLG) producing diverse low molecular weight oligosaccharides
-
-
?
tamarind xyloglucan + H2O
?
3% tamarind xyloglucan
-
-
?
xyloglucan + H2O
?
-
substrate specificity of fungal Xeg1 towards xyloglucan
-
-
?
xyloglucan + H2O
?
-
xyloglucan endotransglucosylase/hydrolases are a family of enzymes that mediate the construction and restructuring of xyloglucan cross-links, thereby controlling the extensibility or mechanical properties of the cell wall in a wide variety of plant tissues
-
-
?
xyloglucan + H2O
?
the GH74 module (PoGH74cat) reveals a highly specific, processive endo-xyloglucanase activity that can hydrolyze the polysaccharide backbone at both branched and unbranched positions
-
-
?
xyloglucan + H2O
?
the GH74 module (PoGH74cat) reveals a highly specific, processive endo-xyloglucanase activity that can hydrolyze the polysaccharide backbone at both branched and unbranched positions
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
source of substrate tamarind seed
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
xyloglucan endotransglucosylase/hydrolase proteins act at the microfibril-matrix interface during cell elongation
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides
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?
xyloglucan + H2O
xyloglucan oligosaccharides
xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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?
xyloglucan + H2O
xyloglucan oligosaccharides
xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides
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?
xyloglucan + H2O
xyloglucan oligosaccharides
Aspergillus fumigatus CBS 101355
xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides
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?
xyloglucan + H2O
xyloglucan oligosaccharides
Aspergillus fumigatus FGSC A1100
xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides
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?
xyloglucan + H2O
xyloglucan oligosaccharides
-
high specific activity towards tamarind xyloglucan
XXXG, XXLG and XLLG oligosaccharides
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?
xyloglucan + H2O
xyloglucan oligosaccharides
substrate xyloglucan from tamarind seed, cleavage at unbranched glucose in the backbone
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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tamarind and pea xyloglucan. The enzyme has at least four subsites (-2 to -2) and specifically recognizes xylose branching at the +1 and +2 sites, branching at the -1 site decreases the activity
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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xyloglucan endotransglucosylase/hydrolase proteins act at the microfibril-matrix interface during cell elongation
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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xyloglucan + H2O
xyloglucan oligosaccharides
xyloglucan from tamarind seeds, structural analysis of xyloglucan binding structure with PoGH74cat, and structural basis of the bond cleavage pattern of PoGH74cat, overview
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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?
xyloglucan + H2O
xyloglucan oligosaccharides
xyloglucan from tamarind seeds, structural analysis of xyloglucan binding structure with PoGH74cat, and structural basis of the bond cleavage pattern of PoGH74cat, overview
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xyloglucan + H2O
xyloglucan oligosaccharides
XEG5 randomly cleaves the xyloglucan main chain
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xyloglucan + H2O
xyloglucan oligosaccharides
XEG74 has dual endo-mode and exo-mode activities
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xyloglucan + H2O
xyloglucan oligosaccharides
XEG74 has dual endo-mode and exo-mode activities
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xyloglucan + H2O
xyloglucan oligosaccharides
XEG5 randomly cleaves the xyloglucan main chain
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xyloglucan + H2O
xyloglucan oligosaccharides
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xyloglucan is a widespread hemicellulose polysaccharide of plant cell walls. It contains mainly D-glucose and D-xylose in the ratio of approximately 4:3, less amount of D-galactose, and also can contain L-fucose and L-arabinose
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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tamarind seed xyloglucan
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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source of substrate: tamarind seed
hydrolysis of unbranched glucose residues. Enzyme initially produces oligosaccharides with a degree of polymerization of 16-18, which are slowly hydrolyzed to final products with a degree of polymerization of 7-9. The ratio of the oligosaccharides with 7-9 to 16-18 glucose units is additionally dependent upon the pH value
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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specifically cleaves the 1,4-beta-glucosyl linkages of the xyloglucan backbone to yield mainly nona- and heptasaccharides
mainly nona- and heptasaccharides
?
xyloglucan + H2O
xyloglucan oligosaccharides
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decreases the weight-average molecular weight of xyloglucans
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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the enzyme depolymerizes xyloglucan more rapidly in the presence than in the absence of xyloglucan oligosaccharides, indicative of a endotransglucosylase activity
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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xyloglucan is a widespread hemicellulose polysaccharide of plant cell walls. It contains mainly D-glucose and D-xylose in the ratio of approximately 4:3, less amount of D-galactose, and also can contain L-fucose and L-arabinose
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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tamarind seed xyloglucan
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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hydrolysis at substituted glucose residues
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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tamarind seed xyloglucan
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?
xyloglucan + H2O
xyloglucan oligosaccharides
the enzyme catalyzes endohydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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decreases the weight-average molecular weight of xyloglucans
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?
xyloglucan + H2O
xyloglucan oligosaccharides
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enzyme has hardly any effect on xyloglucans of less than 60 kDa
products are fragments of about 50 kDa, no production of any oligo- or monosaccharides
?
xyloglucan + H2O
xyloglucan oligosaccharides
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enzyme has no endo-type transglycosylation activity
products are fragments of 5 kDa, no production of oligo- or monosaccharides
?
xyloglucan + H2O
xyloglucooligosaccharides
the endo-xyloglucanase cleaves beta(1->4)-D-glucosidic linkages in the XyG backbone, high specific activity, strong preference for xyloglucan as a natural substrate
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?
xyloglucan + H2O
xyloglucooligosaccharides
the endo-xyloglucanase cleaves beta(1->4)-D-glucosidic linkages in the XyG backbone, high specific activity
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?
xyloglucan + H2O
xyloglucooligosaccharides
the endo-xyloglucanase cleaves beta(1->4)-D-glucosidic linkages in the XyG backbone, high specific activity, strong preference for xyloglucan as a natural substrate
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?
additional information
?
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enzyme does not cleave at substituted glucosyl moieties
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?
additional information
?
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enzyme is involved in protection of the plant against aphids
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?
additional information
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XEG activity affects the cell walls, overview
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additional information
?
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assay method development and evaluation for realtime detection and in vivo measurement of enzyme activity in plant tissue, overview. Substrate is a fluorogenic resorufin beta-glycoside of a xylogluco-oligosaccharide, isolated from Tamarindus indica seed xyloglucans and synthesized as a specific substrate for in planta analysis of XEH activity
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additional information
?
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enzyme XTH15, a classical group-I/II xyloglucan endotransglucosylase/hydrolase (XTH), has high xyloglucan endotransglucosylase (XET) and undetectable xyloglucan hydrolase (XEH) activity in vitro
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additional information
?
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enzyme XTH15, a classical group-I/II xyloglucan endotransglucosylase/hydrolase (XTH), has high xyloglucan endotransglucosylase (XET) and undetectable xyloglucan hydrolase (XEH) activity in vitro
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?
additional information
?
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hydrolysis products formed from [rt-3H]xyloglucan by XTH31 are analysed by GPC
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?
additional information
?
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hydrolysis products formed from [rt-3H]xyloglucan by XTH31 are analysed by GPC
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?
additional information
?
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no activity with beta-glucan and carboxymethylcellulose
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?
additional information
?
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involved in plant cell wall polysaccharide degradation
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?
additional information
?
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no substrate: carboxymethyl cellulose-4M, xylan from birch, beech or oat spelt, lichenan, laminarin, pullulan, galactomannan, glucomannan
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?
additional information
?
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no substrate: carboxymethyl cellulose-4M, xylan from birch, beech or oat spelt, lichenan, laminarin, pullulan, galactomannan, glucomannan
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?
additional information
?
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modelling of substrate binding, overview. Substrate fucosylation does not affect the specific activity of this enzyme. AnXEG12A prefers xylogluco-oligosaccharides containing more than six glucose units, and with xylose substitution at the -3 and +1 subsites, branching structures do not significantly affect the hydrolytic efficiency of the enzyme, overview
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?
additional information
?
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substrate specificity, overview. The enzyme shows no endo-mannanase activity on guar galactomannan and konjac glucomannan, no endoxylanase activity on beechwood xylan and wheat flour arabinoxylan, and no endo-xanthanase on xanthan gum. The recombinant catalytic module indeed demonstrates a strong preference for XyG as a natural substrate. But CjGH74 is unable to release the chromophoric aglycones 2-chloro-4-nitrophenol (CNP) and resorufin from the artificial substrates, XXXG-beta-CNP and XXXG-beta-resorufin. No hydrolysis of the shorter chromogenic substrates CNP-beta-D-cellobioside (GG-beta-CNP) and CNP-beta-D-cellotrioside (GGG-beta-CNP). CjGH74 has an approximately 250 and 970fold higher specificity for xyloglucan compared to the artificial derivative hydroxyethyl cellulose and the mixed-linkage barley-beta-glucan, respectively
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?
additional information
?
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substrate specificity, overview. The enzyme shows no endo-mannanase activity on guar galactomannan and konjac glucomannan, no endoxylanase activity on beechwood xylan and wheat flour arabinoxylan, and no endo-xanthanase on xanthan gum. The recombinant catalytic module indeed demonstrates a strong preference for XyG as a natural substrate. But CjGH74 is unable to release the chromophoric aglycones 2-chloro-4-nitrophenol (CNP) and resorufin from the artificial substrates, XXXG-beta-CNP and XXXG-beta-resorufin. No hydrolysis of the shorter chromogenic substrates CNP-beta-D-cellobioside (GG-beta-CNP) and CNP-beta-D-cellotrioside (GGG-beta-CNP). CjGH74 has an approximately 250 and 970fold higher specificity for xyloglucan compared to the artificial derivative hydroxyethyl cellulose and the mixed-linkage barley-beta-glucan, respectively
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?
additional information
?
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substrate specificity, overview. The enzyme shows no endo-mannanase activity on guar galactomannan and konjac glucomannan, no endoxylanase activity on beechwood xylan and wheat flour arabinoxylan, and no endo-xanthanase on xanthan gum. The recombinant catalytic module indeed demonstrates a strong preference for XyG as a natural substrate. But CjGH74 is unable to release the chromophoric aglycones 2-chloro-4-nitrophenol (CNP) and resorufin from the artificial substrates, XXXG-beta-CNP and XXXG-beta-resorufin. No hydrolysis of the shorter chromogenic substrates CNP-beta-D-cellobioside (GG-beta-CNP) and CNP-beta-D-cellotrioside (GGG-beta-CNP). CjGH74 has an approximately 250 and 970fold higher specificity for xyloglucan compared to the artificial derivative hydroxyethyl cellulose and the mixed-linkage barley-beta-glucan, respectively
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?
additional information
?
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the enzyme encoded by CaXTH1 is involved in the cell expansion process of Cicer arietinum
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?
additional information
?
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no activity with carboxymethylcellulose, Avicel and barley 1,3-1,4-beta-glucan. NO activity with GXXXG and XXXGG. Low activity with GGGX and XXXGX
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?
additional information
?
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role in initiation and elongation of cotton fiber development
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?
additional information
?
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role in initiation and elongation of cotton fiber development
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additional information
?
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role in initiation and elongation of cotton fiber development
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?
additional information
?
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role in initiation and elongation of cotton fiber development
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?
additional information
?
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role in initiation and elongation of cotton fiber development
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?
additional information
?
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role in initiation and elongation of cotton fiber development
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?
additional information
?
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endo-xyloglucanases from GH74 can hydrolyze the regular structure of XXXG-type XyGs at the anomeric center of the unbranched glucosyl (G) unit, although some cleave the backbone at more sterically encumbered positions, e.g. between two X units
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?
additional information
?
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endo-xyloglucanases from GH74 can hydrolyze the regular structure of XXXG-type XyGs at the anomeric center of the unbranched glucosyl (G) unit, although some cleave the backbone at more sterically encumbered positions, e.g. between two X units
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?
additional information
?
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endo-xyloglucanases from GH74 can hydrolyze the regular structure of XXXG-type XyGs at the anomeric center of the unbranched glucosyl (G) unit, although some cleave the backbone at more sterically encumbered positions, e.g. between two X units
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?
additional information
?
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residue Gly476 is uniquely responsible for the promiscuous xyloglucan backbone-cleaving activity of the GH74 module PoGH74cat
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?
additional information
?
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residue Gly476 is uniquely responsible for the promiscuous xyloglucan backbone-cleaving activity of the GH74 module PoGH74cat
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?
additional information
?
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residue Gly476 is uniquely responsible for the promiscuous xyloglucan backbone-cleaving activity of the GH74 module PoGH74cat
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?
additional information
?
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enzymatic assays are performed on AZCL-xyloglucan, release of soluble dye fragments from cross-linked AZCL-XyG is measured. Enzyme PoGH74 shows essentially exclusive specificity for xyloglucan (XyG), which is typical for all GH74 members
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additional information
?
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enzymatic assays are performed on AZCL-xyloglucan, release of soluble dye fragments from cross-linked AZCL-XyG is measured. Enzyme PoGH74 shows essentially exclusive specificity for xyloglucan (XyG), which is typical for all GH74 members
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additional information
?
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enzymatic assays are performed on AZCL-xyloglucan, release of soluble dye fragments from cross-linked AZCL-XyG is measured. Enzyme PoGH74 shows essentially exclusive specificity for xyloglucan (XyG), which is typical for all GH74 members
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additional information
?
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enzymatic assays are performed on AZCL-xyloglucan, release of soluble dye fragments from cross-linked AZCL-XyG is measured. Enzyme PoGH74 shows essentially exclusive specificity for xyloglucan (XyG), which is typical for all GH74 members
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additional information
?
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enzymatic assays are performed on AZCL-xyloglucan, release of soluble dye fragments from cross-linked AZCL-XyG is measured. Enzyme PoGH74 shows essentially exclusive specificity for xyloglucan (XyG), which is typical for all GH74 members
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additional information
?
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endo-xyloglucanases from GH74 can hydrolyze the regular structure of XXXG-type XyGs at the anomeric center of the unbranched glucosyl (G) unit, although some cleave the backbone at more sterically encumbered positions, e.g. between two X units
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?
additional information
?
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endo-xyloglucanases from GH74 can hydrolyze the regular structure of XXXG-type XyGs at the anomeric center of the unbranched glucosyl (G) unit, although some cleave the backbone at more sterically encumbered positions, e.g. between two X units
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?
additional information
?
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residue Gly476 is uniquely responsible for the promiscuous xyloglucan backbone-cleaving activity of the GH74 module PoGH74cat
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?
additional information
?
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residue Gly476 is uniquely responsible for the promiscuous xyloglucan backbone-cleaving activity of the GH74 module PoGH74cat
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?
additional information
?
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no activity with 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta, cellotriose, and cellobiose
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?
additional information
?
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the enzyme demonstrates a broad substrate specificity for polysaccharides containing beta-1,4 linkages and displays an unusual specificity on defined xyloglucan oligosaccharides, cleaving the XXXG-XXXG repeat into XXX and GXXXG
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?
additional information
?
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no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan
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-
?
additional information
?
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no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan
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-
?
additional information
?
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-
no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan
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-
?
additional information
?
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analysis of xyloglucan digestion products by gel-filtration chromatography. Modelling of endo-dissociative- and endo-processive-type xyloglucanase structures, overview
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?
additional information
?
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analysis of xyloglucan digestion products by gel-filtration chromatography. Modelling of endo-dissociative- and endo-processive-type xyloglucanase structures, overview
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?
additional information
?
-
no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan
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-
?
additional information
?
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no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan
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?
additional information
?
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the enzyme is organized in an enzyme complex
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?
additional information
?
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substrate specificity, the enzyme is highly specific for tamarind xyloglucan, it shows no activity against lichenan, methylcellulose, carboxymethyl cellulose, Avicel, xylan from beechwood, xylan from birch wood, barley glucan, laminarin, and galactan as substrates
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?
additional information
?
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substrate specificity, the enzyme is highly specific for tamarind xyloglucan, it shows no activity against lichenan, methylcellulose, carboxymethyl cellulose, Avicel, xylan from beechwood, xylan from birch wood, barley glucan, laminarin, and galactan as substrates
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?
additional information
?
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Penicillium oxalicum HP7-1
substrate specificity, the enzyme is highly specific for tamarind xyloglucan, it shows no activity against lichenan, methylcellulose, carboxymethyl cellulose, Avicel, xylan from beechwood, xylan from birch wood, barley glucan, laminarin, and galactan as substrates
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?
additional information
?
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very low activity against carboxymethyl cellulose, phosphoric acid-swollen cellulose, avicel, glucomannan, galactomannan, and xylan
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?
additional information
?
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enzyme can act on isolated onion epidermis cell wall. Cell wall extension is significantly increased upon addition of enzyme to the isolated epidermis
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?
additional information
?
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involved in tissue softening of ripening fruits
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?
additional information
?
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SIXTH5 has no detectable effect on the mechanical properties of the wall
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?
additional information
?
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enzyme SaGH74A is endo-processive, hydrolysis pattern and mode of action, overview. The C-terminus of enzyme SaGH74A, which is annotated as a carbohydrate-binding module family 2 (CBM2), binds to beta-1,4-linked glucan-containing soluble polysaccharides such as hydroxyethyl cellulose, barley glucan, and xyloglucan, affinities, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
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?
additional information
?
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enzyme SaGH74A is endo-processive, hydrolysis pattern and mode of action, overview. The C-terminus of enzyme SaGH74A, which is annotated as a carbohydrate-binding module family 2 (CBM2), binds to beta-1,4-linked glucan-containing soluble polysaccharides such as hydroxyethyl cellulose, barley glucan, and xyloglucan, affinities, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
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?
additional information
?
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enzyme SaGH74A is endo-processive, hydrolysis pattern and mode of action, overview. The C-terminus of enzyme SaGH74A, which is annotated as a carbohydrate-binding module family 2 (CBM2), binds to beta-1,4-linked glucan-containing soluble polysaccharides such as hydroxyethyl cellulose, barley glucan, and xyloglucan, affinities, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
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?
additional information
?
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enzyme SaGH74B is a typical endo-enzyme, hydrolysis pattern and mode of action, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
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?
additional information
?
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enzyme SaGH74B is a typical endo-enzyme, hydrolysis pattern and mode of action, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
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?
additional information
?
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enzyme SaGH74B is a typical endo-enzyme, hydrolysis pattern and mode of action, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
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?
additional information
?
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enzyme SaGH74B is a typical endo-enzyme, hydrolysis pattern and mode of action, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
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?
additional information
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enzyme SaGH74A is endo-processive, hydrolysis pattern and mode of action, overview. The C-terminus of enzyme SaGH74A, which is annotated as a carbohydrate-binding module family 2 (CBM2), binds to beta-1,4-linked glucan-containing soluble polysaccharides such as hydroxyethyl cellulose, barley glucan, and xyloglucan, affinities, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan)
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XG 25 and XG 70 are organized in an enzyme complex
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enzyme MtXgh74 shows an endoprocessive mode of action. Oligosaccharides XXXG, XXLG, and XLXG are completely hydrolyzed at the end of the reaction, whereas XLLG oligosaccharide is not hydrolyzed
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enzyme MtXgh74 shows an endoprocessive mode of action. Oligosaccharides XXXG, XXLG, and XLXG are completely hydrolyzed at the end of the reaction, whereas XLLG oligosaccharide is not hydrolyzed
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enzyme MtXgh74 shows an endoprocessive mode of action. Oligosaccharides XXXG, XXLG, and XLXG are completely hydrolyzed at the end of the reaction, whereas XLLG oligosaccharide is not hydrolyzed
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Thermothelomyces thermophilus VKPM F-244
enzyme MtXgh74 shows an endoprocessive mode of action. Oligosaccharides XXXG, XXLG, and XLXG are completely hydrolyzed at the end of the reaction, whereas XLLG oligosaccharide is not hydrolyzed
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nasturtium xyloglucanase 1, predominant endo-hydrolase of Tropaeolum majus that can also perform xyloglucan endo-transglycosylation at elevated substrate concentrations
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assay method development and evaluation for realtime detection and in vivo measurement of enzyme activity in plant tissue, overview. Substrate is a fluorogenic resorufin beta-glycoside of a xylogluco-oligosaccharide, isolated from Tamarindus indica seed xyloglucans and synthesized as a specific substrate for in planta analysis of XEH activity
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the enzyme is devoid of transglycosylase activities. There is undetectable activity on cellulose, xylan, arbinoxylan, laminarin, or pectin
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increases the capacity of the cell wall to extend
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cleavage pattern of xyloglucan, overview
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recombinant enzyme in vitro shows xyloglucan endotransglucosylase, but not xyloglucan hydrolase activity
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