3.2.1.151: xyloglucan-specific endo-beta-1,4-glucanase
This is an abbreviated version!
For detailed information about xyloglucan-specific endo-beta-1,4-glucanase, go to the full flat file.
Word Map on EC 3.2.1.151
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3.2.1.151
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xyloglucans
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tamarind
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microfibrils
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xxxg
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expansins
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wall-modifying
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hemicellulosic
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2.4.1.207
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mixed-linkage
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tropaeolum
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nasturtium
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agriculture
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synthesis
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myceliophthora
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degradation
- 3.2.1.151
- xyloglucans
- tamarind
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microfibrils
- xxxg
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expansins
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wall-modifying
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hemicellulosic
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2.4.1.207
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mixed-linkage
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tropaeolum
- nasturtium
- agriculture
- synthesis
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myceliophthora
- degradation
Reaction
Synonyms
AfXEG74, AspGD, AtXTH31, AtXTH32, bacterial glycoside hydrolase family 74 endo-xyloglucanase, BlXG12, CaXTH1, Cel74A, CJA_2477, CjGH74, DkXTH3, DkXTH4, DkXTH5, DkXTH6, DkXTH7, endo-1,4-beta-glucanase/xyloglucanase, endo-beta 1,4 xyloglucan hydrolase, endo-beta-1,4-(xylo)glucan hydrolase, endo-beta-1,4-xyloglucanase, endo-processive xyloglucanase, endo-xyloglucanase, endoxyloglucanase, family GH16 xyloglucan endo-transglycosylase, GH12 xyloglucanase, GH16 xyloglucan hydrolase, GH74 endo-xyloglucanase, GH74 xyloglucanase, Gly74A, GT77, LeXTH1, More, Mt-XTH1, MtXgh74, MYCTH_116384, NXG1, oligoxyloglucan hydrolase, PODO_RS11130, PoGH74, POXEG12A, PoxXEG12A, SaGH74A, SaGH74B, SIXTH5, TmNXG1, TXy, XcXGHA, XEG, Xeg1, XEG12A, XEG5, XEG5A, XEG74, XegA, XEH, XET, XG 25, XG 70, XG A, XG-ase, XG12, XG44, XG5, XGH74, Xgl74A, XH, XTH, XTH1, XTH15, XTH2, XTH3, XTH31, XTH4, XTH6, XTH7, xyloglucan endo-beta-1,4-glucanase, xyloglucan endohydrolase, xyloglucan endotransglucosylase/hydrolase, xyloglucan endotransglucosylase/hydrolase 3, xyloglucan endotransglucosylase/hydrolase protein 31, xyloglucan endotransglucosylase/hydrolase protein 32, xyloglucan endotransglucosylase/hydrolases, xyloglucan endotransglycosylase/hydrolase, xyloglucan hydrolase, xyloglucan-specific beta-(1,4)-glucanase, xyloglucan-specific endo 1,4-beta-glucanase, xyloglucan-specific endo-1,4beta-glucanase, xyloglucan-specific endo-beta-1,4-glucanase, xyloglucan-specific family 12 hydrolase, xyloglucanase, xyloglucanase 1, xyloglucanase A, xyloglucanendohydrolase, xyloglycan hydrolase
ECTree
3.2.1.151 xyloglucan-specific endo-beta-1,4-glucanaseAdvanced search results
results (
results (Engineering
Engineering on EC 3.2.1.151 - xyloglucan-specific endo-beta-1,4-glucanase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D74A
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crystallization data in complex with oligosaccharide substrate
E155A
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the mutant acts as glucosynthase and can perform the condensation of xyloglucosyl fluorides, albeit at poor rates
D483A
site-directed mutagenesis, a catalytic acid mutant, the mutation causes loss of the enzymatic activity by more than 10 000fold compared to the wild-type enzyme
D70A
site-directed mutagenesis, the mutation causes loss of the enzymatic activity by more than 10 000fold compared to the wild-type enzyme
D483A
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site-directed mutagenesis, a catalytic acid mutant, the mutation causes loss of the enzymatic activity by more than 10 000fold compared to the wild-type enzyme
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D70A
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site-directed mutagenesis, the mutation causes loss of the enzymatic activity by more than 10 000fold compared to the wild-type enzyme
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W13A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
W13A/W28A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
W28A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
Y24A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
D70A
G476Y
W347A
W348A
W406A
Y372A
D70A
G476Y
W347A
Y372A
W318A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
W319A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
W61A
site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme
W64A
site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme
W318A
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site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
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W319A
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site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
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W61A
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site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme
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W64A
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site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme
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DELTAYNIIG
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loop deletion variant of isoform NXG1, structurally similar to the strict endo-transglycolase from Populus tremula x Populus tremuloides. Mutant has a greatly increased transglycosylation:hydrolysis ratio
E94A
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the GH16 xyloglucan hydrolase mutant of TmNXG1 acting as glycosynthase is capable of synthesizing XLLG-based xyloglucan oligosaccharides at rates feasible for preparative synthesis, thus providing an essential expansion of product ranges
additional information
D70A
site-directed mutagenesis in the PoGH74cat module at the catalytic base
D70A
site-directed mutagenesis, mutation of the catalytic base, the mutant turnover rate is similar to wild-type
G476Y
site-directed mutagenesis in the PoGH74cat module at the -1 subsite
G476Y
site-directed mutagenesis, mutation of the -1 subsite, the mutant turnover rate is similar to wild-type
W347A
site-directed mutagenesis in the PoGH74cat module at the +3 subsite
W347A
site-directed mutagenesis, mutation of the +3 subsite, kcat value is increased by 5fold compared to wild-type
W348A
site-directed mutagenesis in the PoGH74cat module at the +5 subsite
W348A
site-directed mutagenesis, mutation of the +5 subsite, the mutant turnover rate is similar to wild-type
W406A
site-directed mutagenesis in the PoGH74cat module at the +2 subsite
W406A
site-directed mutagenesis, mutation of the +2 subsite, the mutant turnover rate is similar to wild-type
Y372A
site-directed mutagenesis in the PoGH74cat module at the +6 subsite
Y372A
site-directed mutagenesis, mutation of the +6 subsite, the mutant turnover rate is similar to wild-type
D70A
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site-directed mutagenesis, mutation of the catalytic base, the mutant turnover rate is similar to wild-type
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D70A
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site-directed mutagenesis in the PoGH74cat module at the catalytic base
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G476Y
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site-directed mutagenesis, mutation of the -1 subsite, the mutant turnover rate is similar to wild-type
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G476Y
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site-directed mutagenesis in the PoGH74cat module at the -1 subsite
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W347A
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site-directed mutagenesis, mutation of the +3 subsite, kcat value is increased by 5fold compared to wild-type
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W347A
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site-directed mutagenesis in the PoGH74cat module at the +3 subsite
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Y372A
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site-directed mutagenesis, mutation of the +6 subsite, the mutant turnover rate is similar to wild-type
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Y372A
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site-directed mutagenesis in the PoGH74cat module at the +6 subsite
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additional information
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enzyme deletion mutant. Myzus persicae aphids settle preferentially on the mutant rather than on the wild-type. Ectopic expression of enzyme in the phloem is not sufficient to confer protection
additional information
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loss-of-function mutant of isoform XTH21. Compared with wild type, root hairs and root cells of xth21 are shorter and cell shapes are anomalous
additional information
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atxth28: null mutant with insert blocking gene expression of AtXTH28, shorter proximal siliques with fewer seeds, reduced self-pollination, atxth28/AtXTH28: transgenic mutant with insertion of intact AtXTH28 fragment into atxth28 null-mutant, wild type silique growth and self-pollination ability restored, atxth27/atxth28: double-null-mutant, no additional phenotype expression compared to atxth28 null-mutant
additional information
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construction of random XEG T-DNA insertion mutants, dozens of plant cell wall xeg mutants are identified, leading to the identification of 23 genetic loci that affect plant cell walls. One of the identified loci is XEG113, encoding a family 77 glycosyltransferase, GT77. Detailed analysis of the wall of this mutant indicates that its extensins, structural glyocoproteins present in walls, are underarabinosylated. Xeg-113 plants exhibit more elongated hypocotyls than wild-type, mutant cell wall composition and phenotype, overview
additional information
contruction of XTH31 single and double knockout lines with gene XTH32, the enzyme hydrolytic activity is essentially completely eliminated in AtXTH31/AtXTH32 double knockout lines
additional information
contruction of XTH31 single and double knockout lines with gene XTH32, the enzyme hydrolytic activity is essentially completely eliminated in AtXTH31/AtXTH32 double knockout lines
additional information
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contruction of XTH31 single and double knockout lines with gene XTH32, the enzyme hydrolytic activity is essentially completely eliminated in AtXTH31/AtXTH32 double knockout lines
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additional information
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mutation and engineering of wild-type xyloglucanase to glycosynthases, hydrolytically inactive mutant glycosidases that catalyze glycosylation reactions using glycosyl fluoride donor substrates
additional information
transgenic expression in Arabidopsis thaliana leads to abnormal leaf morphology with twisting and bending along the edges. Leaves show increased numbers of small-sized cells, resulting in disordered, highly populated mesophyll cells in each dorsoventral layer containing a limited amount of starch. Transgenic plants display a markedly improved tolerance to severe water deficit, and to a lesser extent to high salinity in comparison with the wild-type plants
additional information
the full-length CJA_2477 gene product is composed of a signal peptide, a GH74 catalytic domain and two carbohydrate binding modules: CBM10 and CBM2. The GH74, CBM10 and CBM2 modules are connected by serine rich linkers. Construction of truncated enzyme forms and isolated domains and recombinant expression as His- and GFP-tagged proteins in Escherichia coli
additional information
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the full-length CJA_2477 gene product is composed of a signal peptide, a GH74 catalytic domain and two carbohydrate binding modules: CBM10 and CBM2. The GH74, CBM10 and CBM2 modules are connected by serine rich linkers. Construction of truncated enzyme forms and isolated domains and recombinant expression as His- and GFP-tagged proteins in Escherichia coli
additional information
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the full-length CJA_2477 gene product is composed of a signal peptide, a GH74 catalytic domain and two carbohydrate binding modules: CBM10 and CBM2. The GH74, CBM10 and CBM2 modules are connected by serine rich linkers. Construction of truncated enzyme forms and isolated domains and recombinant expression as His- and GFP-tagged proteins in Escherichia coli
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additional information
generation of PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675) truncated mutants
additional information
generation of PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675) truncated mutants
additional information
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generation of PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675) truncated mutants
additional information
construction of truncated mutants, i.e. PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675), the mutant turnover rate is similar to wild-type
additional information
construction of truncated mutants, i.e. PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675), the mutant turnover rate is similar to wild-type
additional information
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construction of truncated mutants, i.e. PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675), the mutant turnover rate is similar to wild-type
additional information
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construction of truncated mutants, i.e. PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675), the mutant turnover rate is similar to wild-type
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additional information
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generation of PoGH74cat(DELTA642-651) and PoGH74cat(DELTA671-675) truncated mutants
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additional information
selective replacement of the positive subsite residues with alanine mutations reduces the degree of processive activity and resulted in the more endo-dissociative-activity
additional information
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selective replacement of the positive subsite residues with alanine mutations reduces the degree of processive activity and resulted in the more endo-dissociative-activity
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additional information
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overexpression of the enzyme in poplar trees leads to xylem and xyloglucan degradation accelerating cellulose saccharification. The crystalline region of the cellulose microfibrils is highly degraded in the xylem overexpressing xyloglucanase comapred to overexpression of cellulase, xylanase, or galactanase, overview
additional information
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TmNXG1-deltaYNIIG: active site loop deletion mutant, three loops: Asn84-Asp93 (glycosyl donor subsite), Glu117-Gly126, and Trp190-Tyr197 (critical to acceptor substrate binding, inter alia influencing transglycosylation/hydrolysis ratio)
additional information
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mutation and engineering of wild-type xyloglucanase to glycosynthases, hydrolytically inactive mutant glycosidases that catalyze glycosylation reactions using glycosyl fluoride donor substrates
additional information
Arabidopsis thaliana plants expressing the enzyme show slightly increased xyloglucan endohydrolase activity and alterations in the cell wall structure and composition
