Cloned (Comment) | Organism |
---|---|
expression of GST-tagged enzyme in Escherichia coli BL21 | Daucus carota |
Crystallization (Comment) | Organism |
---|---|
purified enzyme free or in complex with substrate xyloglucan or inhibitor extracellular dermal glycoprotein, hanging drop vapor diffusion method, from 0.1 M sodium acetate, pH 5.5, and 1.5 M ammonium sulfate, or 0.1 M sodium acetate, pH 4.6, 25% PEG 3000, and 5 mg/ml digested xyloglucan, 20°C, X-ray diffraction structure determination and analysis at 0.95-2.70 A resolution | Daucus carota |
Protein Variants | Comment | Organism |
---|---|---|
W13A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Daucus carota |
W13A/W28A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Daucus carota |
W28A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Daucus carota |
Y24A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Daucus carota |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
extracellular dermal glycoprotein | a glycoside hydrolase inhibitor protein inhibitor of the enzyme, it closely mimics the XEG-xyloglucan interaction | Daucus carota |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota | Q05929 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli BL21 by glutathione affinity chromatography and gel filtration | Daucus carota |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tamarind seed xyloglucan + H2O | - |
Daucus carota | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XEG | - |
Daucus carota |
xyloglucanase | - |
Daucus carota |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Daucus carota |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
assay at | Daucus carota |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of GH family 12, GH12 | Daucus carota |
physiological function | xyloglucan is a major component of the cell wall in most plants, and xyloglucan-specific endo-beta-1,4-glucanase digests the polysaccharide chains of plant cell walls. To protect the cell wall from these enzymes, plants secrete glycoside hydrolase inhibitor proteins | Daucus carota |