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3.1.21.8: T4 deoxyribonuclease II

This is an abbreviated version!
For detailed information about T4 deoxyribonuclease II, go to the full flat file.

Word Map on EC 3.1.21.8

Reaction

endonucleolytic nicking and cleavage of cytosine-containing double-stranded DNA =

Synonyms

denA, EndoII, endonuclease II, endonuclease IV, GIY-YIG enzyme, T4 endonuclease II

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters
                3.1.21.8 T4 deoxyribonuclease II

Engineering

Engineering on EC 3.1.21.8 - T4 deoxyribonuclease II

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A83L
Tequatrovirus T4
similar to wild-type, cleavage activity is 20 to 70% of the nicking activity
E118A
I24A
Tequatrovirus T4
similar to wild-type, cleavage activity is 20 to 70% of the nicking activity
K12A
Tequatrovirus T4
similar to wild-type, cleavage activity is 20 to 70% of the nicking activity
K76A 
Tequatrovirus T4
much reduced catalytic activity
L16A
Tequatrovirus T4
similar to wild-type, cleavage activity is 20 to 70% of the nicking activity
L84P
Tequatrovirus T4
much reduced catalytic activity
N130A
Tequatrovirus T4
complete loss of cleavage activity
P127L
Tequatrovirus T4
much reduced catalytic activity
S72A
Tequatrovirus T4
similar to wild-type, cleavage activity is 20 to 70% of the nicking activity
V90A
Tequatrovirus T4
similar to wild-type, cleavage activity is 20 to 70% of the nicking activity