3.1.21.8: T4 deoxyribonuclease II

This is an abbreviated version!
For detailed information about T4 deoxyribonuclease II, go to the full flat file.

Word Map on EC 3.1.21.8

3.1.21.8

bacteriophage

nick

phage

strands

single-stranded

endonucleases

coliphage

i-tevi

cytosine-containing

cytosine

hydroxymethylase

dcmp

exonuclease

scissile

dctpase

Reaction

endonucleolytic nicking and cleavage of cytosine-containing double-stranded DNA =

Synonyms

denA, EndoII, endonuclease II, endonuclease IV, GIY-YIG enzyme, T4 endonuclease II

ECTree

3 Hydrolases

3.1 Acting on ester bonds

3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters

3.1.21.8 T4 deoxyribonuclease II

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Results	in table
8	AA Sequence
3	Cloned(Commentary)
2	Crystallization (Commentary)
1	General Information
3	Inhibitors
5	Metals/Ions
2	Natural Substrates/ Products (Substrates)
8	Organism
1	pH Optimum
17	Protein Variants
1	Reaction
8	Reference
6	Substrates and Products (Substrate)
9	Synonyms
1	Temperature Optimum [°C]
1	Temperature Stability [°C]

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Reference on EC 3.1.21.8 - T4 deoxyribonuclease II

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666387

Carlson, K.; Lagerback, P.; Nystrom, A.C.

Bacteriophage T4 endonuclease II: concerted single-strand nicks yield double-strand cleavage

Mol. Microbiol.

1403-1411

2004

Tequatrovirus T4 (P07059)

15165242

715906

Andersson, C.E.; Lagerbaeck, P.; Carlson, K.

Structure of bacteriophage T4 endonuclease II mutant E118A, a tetrameric GIY-YIG enzyme

J. Mol. Biol.

397

1003-1016

2010

Tequatrovirus T4 (P07059)

20156453

725989

Carlson, K.; Kosturko, L.D.; Nystrom, A.C.

Sequence-specific cleavage by bacteriophage T4 endonuclease II in vitro

Mol. Microbiol.

1395-1405

1999

Tequatrovirus T4 (P07059)

10200960

727596

Carlson, K.; Overvatn, A.

Bacteriophage T4 endonucleases II and IV, oppositely affected by dCMP hydroxymethylase activity, have different roles in the degradation and in the RNA polymerase-dependent replication of T4 cytosine-containing DNA

Genetics

114

669-685

1986

Tequatrovirus T4 (P07059)

2431959

727747

Lagerbaeck, P.; Carlson, K.

Amino acid residues in the GIY-YIG endonuclease II of phage T4 affecting sequence recognition and binding as well as catalysis

J. Bacteriol.

190

5533-5544

2008

Tequatrovirus T4 (P07059)

18539732

727809

Krabbe, M.; Carlson, K.

In vivo restriction. Sequence and structure of endonuclease II-dependent cleavage sites in bacteriophage T4 DNA

J. Biol. Chem.

266

23407-23415

1991

Tequatrovirus T4 (P07059)

1744134

727812

Carlson, K.; Krabbe, M.; Nystroem, A.C.; Kosturko, L.D.

DNA determinants of restriction. Bacteriophage T4 endonuclease II-dependent cleavage of plasmid DNA in vivo

J. Biol. Chem.

268

8908-8918

1993

Tequatrovirus T4 (P07059)

8386173

728389

Lagerbaeck, P.; Andersson, E.; Malmberg, C.; Carlson, K.

Bacteriophage T4 endonuclease II, a promiscuous GIY-YIG nuclease, binds as a tetramer to two DNA substrates

Nucleic Acids Res.

6174-6183

2009

Tequatrovirus T4 (P07059)

19666720