3.1.21.8: T4 deoxyribonuclease II

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For detailed information about T4 deoxyribonuclease II, go to the full flat file.

Reaction

endonucleolytic nicking and cleavage of cytosine-containing double-stranded DNA =

Synonyms

denA, EndoII, endonuclease II, endonuclease IV, GIY-YIG enzyme, T4 endonuclease II

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters

                3.1.21.8 T4 deoxyribonuclease II

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Results	in table
8	AA Sequence
3	Cloned(Commentary)
2	Crystallization (Commentary)
1	General Information
3	Inhibitors
5	Metals/Ions
2	Natural Substrates/ Products (Substrates)
8	Organism
1	pH Optimum
17	Protein Variants
1	Reaction
8	Reference
6	Substrates and Products (Substrate)
9	Synonyms
1	Temperature Optimum [°C]
1	Temperature Stability [°C]

Metals Ions

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Metals Ions on EC 3.1.21.8 - T4 deoxyribonuclease II

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Mg2+

Tequatrovirus T4

P07059

required

725989, 727747

Mn2+

Tequatrovirus T4

P07059

compared to Mg2+, relative nicking activity is 0.4 with top strand, 0.8 with bottom strand

727747

Ni2+

Tequatrovirus T4

P07059

compared to Mg2+, relative nicking activity is 0.1 with top strand, 0.1 with bottom strand

727747

additional information

Tequatrovirus T4

P07059

ca2+ cannot substitute Mg2+

727747