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2.1.1.85: protein-histidine N-methyltransferase

This is an abbreviated version!
For detailed information about protein-histidine N-methyltransferase, go to the full flat file.

Reaction

S-adenosyl-L-methionine
+
protein L-histidine
=
S-adenosyl-L-homocysteine
+
protein Ntau-methyl-L-histidine

Synonyms

actin His73 nmethyltransferase, actin histidine methyltransferase, actin histidine-N3 methyltransferase, actin-specific histidine methyltransferase, actin-specific histidine N-methyltransferase, actin-specific methyltransferase, methyltransferase, protein (histidine), protein (actin) histidine MTase, protein methylase IV, S-adenosyl methionine:protein-histidine N-methyltransferase, SET domain protein 3, setd3

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.85 protein-histidine N-methyltransferase

Crystallization

Crystallization on EC 2.1.1.85 - protein-histidine N-methyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme in complex with an actin peptide (residues 66-80) that contains His73 in the presence of S-adenosyl-L-homocysteine, hanging drop vapor diffusion method, using either 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000, or 0.2 M ammonium sulfate or 0.2 M sodium chloride, 0.1 M Bis-Tris pH 5.5, 25% (w/v) polyethylene glycol 3350 and 0.1 M Bis-Tris pH 5.5, 25% (w/v) polyethylene glycol 3350
S-adenosyl-L-homocysteine-bound enzyme in complex with either an unmodified beta-actin peptide or its His-methylated variant, sitting drop vapor diffusion method, using 0.1 M sodium cacodylate trihydrate (pH 6.5), 0.2 M magnesium acetate tetrahydrate, and 20% (w/v) polyethylene glycol 8000
sitting drop vapor diffusion method, using 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000