Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
S-adenosyl-L-homocysteine-bound enzyme in complex with either an unmodified beta-actin peptide or its His-methylated variant, sitting drop vapor diffusion method, using 0.1 M sodium cacodylate trihydrate (pH 6.5), 0.2 M magnesium acetate tetrahydrate, and 20% (w/v) polyethylene glycol 8000 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
N256A | the mutant shows severely reduced activity compared to the wild type enzyme | Homo sapiens |
N256D | the mutant shows severely reduced activity compared to the wild type enzyme | Homo sapiens |
N256Q | the mutant shows about 50% activity compared to the wild type enzyme | Homo sapiens |
N278A | the mutant shows severely reduced activity compared to the wild type enzyme | Homo sapiens |
R215A | the mutant shows severely reduced activity compared to the wild type enzyme | Homo sapiens |
R316A | the mutant shows severely reduced activity compared to the wild type enzyme | Homo sapiens |
R75A | the mutant shows severely reduced activity compared to the wild type enzyme | Homo sapiens |
Y313F | the mutant shows severely reduced activity compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000047 | - |
beta-actin L-histidine73 | mutant enzyme N278A, at pH 7.2 and 37°C | Homo sapiens | |
0.000103 | - |
S-adenosyl-L-methionine | mutant enzyme N256Q, at pH 7.2 and 37°C | Homo sapiens | |
0.000111 | - |
S-adenosyl-L-methionine | wild type enzyme, at pH 7.2 and 37°C | Homo sapiens | |
0.000149 | - |
S-adenosyl-L-methionine | mutant enzyme R316A, at pH 7.2 and 37°C | Homo sapiens | |
0.000165 | - |
S-adenosyl-L-methionine | mutant enzyme N256D, at pH 7.2 and 37°C | Homo sapiens | |
0.000165 | - |
S-adenosyl-L-methionine | mutant enzyme R215A, at pH 7.2 and 37°C | Homo sapiens | |
0.000305 | - |
beta-actin L-histidine73 | mutant enzyme N256D, at pH 7.2 and 37°C | Homo sapiens | |
0.00035 | - |
S-adenosyl-L-methionine | mutant enzyme N278A, at pH 7.2 and 37°C | Homo sapiens | |
0.000449 | - |
beta-actin L-histidine73 | mutant enzyme N256A, at pH 7.2 and 37°C | Homo sapiens | |
0.000502 | - |
beta-actin L-histidine73 | wild type enzyme, at pH 7.2 and 37°C | Homo sapiens | |
0.000565 | - |
beta-actin L-histidine73 | mutant enzyme R75A, at pH 7.2 and 37°C | Homo sapiens | |
0.000581 | - |
beta-actin L-histidine73 | mutant enzyme Y313F, at pH 7.2 and 37°C | Homo sapiens | |
0.000686 | - |
S-adenosyl-L-methionine | mutant enzyme N256A, at pH 7.2 and 37°C | Homo sapiens | |
0.000784 | - |
beta-actin L-histidine73 | mutant enzyme N256Q, at pH 7.2 and 37°C | Homo sapiens | |
0.001087 | - |
beta-actin L-histidine73 | mutant enzyme R215A, at pH 7.2 and 37°C | Homo sapiens | |
0.001115 | - |
beta-actin L-histidine73 | mutant enzyme R316A, at pH 7.2 and 37°C | Homo sapiens | |
0.003601 | - |
S-adenosyl-L-methionine | mutant enzyme R75A, at pH 7.2 and 37°C | Homo sapiens | |
0.006543 | - |
S-adenosyl-L-methionine | mutant enzyme Y313F, at pH 7.2 and 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + beta-actin L-histidine73 | Homo sapiens | - |
S-adenosyl-L-homocysteine + beta-actin Ntau-methyl-L-histidine73 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q86TU7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and HisTrap column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with histone H3 L-lysine4 (1-23), histone H3 L-lysine36 (25-47), and beta-actin (66-88) H73A mutant | Homo sapiens | ? | - |
- |
|
S-adenosyl-L-methionine + beta-actin L-histidine73 | - |
Homo sapiens | S-adenosyl-L-homocysteine + beta-actin Ntau-methyl-L-histidine73 | - |
? | |
S-adenosyl-L-methionine + synthetic peptide corresponding to residue 66-88 of beta-actin | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
actin-specific methyltransferase | - |
Homo sapiens |
setd3 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000017 | - |
S-adenosyl-L-methionine | mutant enzyme N278A, at pH 7.2 and 37°C | Homo sapiens | |
0.000017 | - |
beta-actin L-histidine73 | mutant enzyme N278A, at pH 7.2 and 37°C | Homo sapiens | |
0.000033 | - |
beta-actin L-histidine73 | mutant enzyme R75A, at pH 7.2 and 37°C | Homo sapiens | |
0.00027 | - |
S-adenosyl-L-methionine | mutant enzyme R75A, at pH 7.2 and 37°C | Homo sapiens | |
0.00037 | - |
beta-actin L-histidine73 | mutant enzyme Y313F, at pH 7.2 and 37°C | Homo sapiens | |
0.00052 | - |
beta-actin L-histidine73 | mutant enzyme N256A, at pH 7.2 and 37°C | Homo sapiens | |
0.00067 | - |
S-adenosyl-L-methionine | mutant enzyme N256D, at pH 7.2 and 37°C | Homo sapiens | |
0.00097 | - |
beta-actin L-histidine73 | mutant enzyme N256D, at pH 7.2 and 37°C | Homo sapiens | |
0.0011 | - |
S-adenosyl-L-methionine | mutant enzyme N256A, at pH 7.2 and 37°C | Homo sapiens | |
0.0016 | - |
S-adenosyl-L-methionine | mutant enzyme Y313F, at pH 7.2 and 37°C | Homo sapiens | |
0.0029 | - |
beta-actin L-histidine73 | mutant enzyme R316A, at pH 7.2 and 37°C | Homo sapiens | |
0.0035 | - |
beta-actin L-histidine73 | mutant enzyme R215A, at pH 7.2 and 37°C | Homo sapiens | |
0.00365 | - |
S-adenosyl-L-methionine | mutant enzyme R215A, at pH 7.2 and 37°C | Homo sapiens | |
0.0041 | - |
S-adenosyl-L-methionine | mutant enzyme R316A, at pH 7.2 and 37°C | Homo sapiens | |
0.0063 | - |
S-adenosyl-L-methionine | mutant enzyme N256Q, at pH 7.2 and 37°C | Homo sapiens | |
0.011 | - |
beta-actin L-histidine73 | mutant enzyme N256Q, at pH 7.2 and 37°C | Homo sapiens | |
0.0112 | - |
S-adenosyl-L-methionine | wild type enzyme, at pH 7.2 and 37°C | Homo sapiens | |
0.013 | - |
beta-actin L-histidine73 | wild type enzyme, at pH 7.2 and 37°C | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays important roles in hypoxic pulmonary hypertension, muscle differentiation, and carcinogenesis | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
S-adenosyl-L-methionine | mutant enzyme N278A, at pH 7.2 and 37°C | Homo sapiens | |
0.067 | - |
S-adenosyl-L-methionine | mutant enzyme R75A, at pH 7.2 and 37°C | Homo sapiens | |
0.067 | - |
beta-actin L-histidine73 | mutant enzyme R75A, at pH 7.2 and 37°C | Homo sapiens | |
0.25 | - |
S-adenosyl-L-methionine | mutant enzyme Y313F, at pH 7.2 and 37°C | Homo sapiens | |
0.35 | - |
beta-actin L-histidine73 | mutant enzyme N278A, at pH 7.2 and 37°C | Homo sapiens | |
0.63 | - |
beta-actin L-histidine73 | mutant enzyme Y313F, at pH 7.2 and 37°C | Homo sapiens | |
1.15 | - |
beta-actin L-histidine73 | mutant enzyme N256A, at pH 7.2 and 37°C | Homo sapiens | |
1.65 | - |
S-adenosyl-L-methionine | mutant enzyme N256A, at pH 7.2 and 37°C | Homo sapiens | |
2.58 | - |
beta-actin L-histidine73 | mutant enzyme R316A, at pH 7.2 and 37°C | Homo sapiens | |
3.17 | - |
beta-actin L-histidine73 | mutant enzyme N256D, at pH 7.2 and 37°C | Homo sapiens | |
3.25 | - |
beta-actin L-histidine73 | mutant enzyme R215A, at pH 7.2 and 37°C | Homo sapiens | |
4.03 | - |
S-adenosyl-L-methionine | mutant enzyme N256D, at pH 7.2 and 37°C | Homo sapiens | |
13.8 | - |
beta-actin L-histidine73 | mutant enzyme N256Q, at pH 7.2 and 37°C | Homo sapiens | |
22.12 | - |
S-adenosyl-L-methionine | mutant enzyme R215A, at pH 7.2 and 37°C | Homo sapiens | |
26.87 | - |
beta-actin L-histidine73 | wild type enzyme, at pH 7.2 and 37°C | Homo sapiens | |
27.3 | - |
S-adenosyl-L-methionine | mutant enzyme R316A, at pH 7.2 and 37°C | Homo sapiens | |
61.48 | - |
S-adenosyl-L-methionine | mutant enzyme N256Q, at pH 7.2 and 37°C | Homo sapiens | |
101.05 | - |
S-adenosyl-L-methionine | wild type enzyme, at pH 7.2 and 37°C | Homo sapiens |