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1.13.11.2: catechol 2,3-dioxygenase

This is an abbreviated version!
For detailed information about catechol 2,3-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.2

Reaction

catechol
+
O2
=
2-hydroxymuconate-6-semialdehyde

Synonyms

2,3-dihydroxybenzenesulfonate 2,3-dioxygenase, 3-sulfocatechol-2,3-dioxygenase, 3SC23O, A23O, AtdB, BupB, C2,3O, C23D, C23o, C23O-2G, C23O1, C23O2, C23Os, CatE, catechol 2,3 dioxygenase, catechol 2,3-di-2,3-pyrocatechase, catechol 2,3-dioxygenase, catechol 2,3-oxygenase, catechol oxygenase, catechol-2,3-dioxygenase, CbzE, CD-2,3, EC 1.13.1.2, ECDO, extradiol dioxygenase MhpB, Extradiol-cleaving catecholic dioxygenase, meta-cleavage dioxygenase, metapyrocatechase, More, oxygenase, PheB, pyrocatechol 2,3-dioxygenase, Saci_2295, SSO1223, SsoC2,3O, ssol_2912, tbuE, TdnC, TodE, XylE

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.2 catechol 2,3-dioxygenase

Cloned

Cloned on EC 1.13.11.2 - catechol 2,3-dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned by PCR technique, and linked to suicide vector pUTmini-Tn5, then the recombinant vector pUTmini-Tn5-C23O is obtained. The C230 gene of pUTmini-Tn5-C23O is integrated into the chromosomal DNA of Pseudomonas sp. wp4 with the triparental conjugation effect of mini-Tn5 transposition, and finally the gene engineering bacteria wp4-C23O is obtained
-
cloned in Escherichia coli DH5alpha
DNA and amino acid sequence determination and analysis
expressed in Acinetobacter sp. BS3
-
expressed in Escherichia coli
expressed in Escherichia coli (JM109)
-
expressed in Escherichia coli BL21 (DE3)
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH 5alpha
-
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli JM107 cells
expressed in Escherichia coli JM109
expressed in Escherichia coli strain BL21(DE3)
-
expressed in Escherichia coli strains JM109 and DH5alpha
-
expressed in Pseudomonas putida G7.C-1 and Escherichia coli DH5alpha
expression in Escherichia coli
expression in Escherichia coli BL21
-
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli LE392
expression in Escherichia coli TG1
-
expression in Escherichia coli W3110
-
gene c23o, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes and recombinant expression in Escherichia coli strain BL21
-
gene nahH, DNA and amino acid sequence determination and analysis, phylogenetic tree
overexpression in Escherichia coli BL21
-