Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas sp. |
expressed in Escherichia coli | Sphingomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of different hybrid enzymes containing different parts of type I.2.A and I.2.B enzyme, only one of the constructed enzymes is found to be active | Pseudomonas sp. |
additional information | construction of different hybrid enzymes containing different parts of type I.2.A and I.2.B enzyme, only one of the constructed enzymes is found to be active | Sphingomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
catechol | native enzyme, pH 7.5, 25°C | Pseudomonas sp. | |
0.0038 | - |
catechol | native enzyme, pH 7.5, 25°C | Sphingomonas sp. | |
0.02 | - |
catechol | hybrid enzyme, pH 7.5, 25°C | Pseudomonas sp. | |
0.02 | - |
catechol | hybrid enzyme, pH 7.5, 25°C | Sphingomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | bound to protein | Pseudomonas sp. | |
Iron | bound to protein | Sphingomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
4 * 37000, SDS-PAGE | Pseudomonas sp. |
37000 | - |
4 * 37000, SDS-PAGE | Sphingomonas sp. |
150000 | - |
gel filtration | Pseudomonas sp. |
150000 | - |
gel filtration | Sphingomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | Pseudomonas sp. | key enzyme for the degradation of aromatic compounds | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | Sphingomonas sp. | key enzyme for the degradation of aromatic compounds | 2-hydroxymuconate semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
enzyme subgroup I.2.A, hybrid enzymes with type I.2.B enzyme from Sphingomonas sp. | - |
Sphingomonas sp. | - |
enzyme subgroup I.2.B, hybrid enzymes with type I.2.A enzyme from Pseudomonas sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | - |
Pseudomonas sp. | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | - |
Sphingomonas sp. | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | key enzyme for the degradation of aromatic compounds | Pseudomonas sp. | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | key enzyme for the degradation of aromatic compounds | Sphingomonas sp. | 2-hydroxymuconate semialdehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 37000, SDS-PAGE | Pseudomonas sp. |
tetramer | 4 * 37000, SDS-PAGE | Sphingomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
148 | - |
catechol | hybrid enzyme, pH 7.5, 25°C | Pseudomonas sp. | |
148 | - |
catechol | hybrid enzyme, pH 7.5, 25°C | Sphingomonas sp. | |
238 | - |
catechol | native enzyme, pH 7.5, 25°C | Sphingomonas sp. | |
493 | - |
catechol | native enzyme, pH 7.5, 25°C | Pseudomonas sp. |