1.13.11.2: catechol 2,3-dioxygenase
This is an abbreviated version!
For detailed information about catechol 2,3-dioxygenase, go to the full flat file.
Word Map on EC 1.13.11.2
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1.13.11.2
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heme
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putida
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carboxylase
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hydroxylase
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rubisco
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ferredoxins
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ribulose-1,5-bisphosphate
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l-arginine
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non-heme
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rieske
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tetrahydrobiopterin
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dioxygenation
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ribulose
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toluene
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2-oxoglutarate
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naphthalene
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biphenyls
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rhodococcus
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fmn
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nnos
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bioremediation
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mixed-function
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nitric-oxide
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phenanthrene
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photorespiration
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photorespiratory
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1,5-bisphosphate
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biliverdin
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bh4
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l-citrulline
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sphingomonas
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ring-hydroxylating
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comamonas
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dihydroxylation
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carbazole
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xylene
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l-arg
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2-oxoglutarate-dependent
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heme-heme
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diiron
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ironii
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4-methylcatechol
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monooxygenation
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rubp
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testosteroni
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sphingobium
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ethylbenzene
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4.1.1.39
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degradation
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environmental protection
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fluoranthene
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rhodochrous
- 1.13.11.2
- heme
- putida
- carboxylase
- hydroxylase
- rubisco
- ferredoxins
- ribulose-1,5-bisphosphate
- l-arginine
-
non-heme
-
rieske
- tetrahydrobiopterin
-
dioxygenation
- ribulose
- toluene
- 2-oxoglutarate
- naphthalene
- biphenyls
- rhodococcus
- fmn
- nnos
-
bioremediation
-
mixed-function
-
nitric-oxide
- phenanthrene
-
photorespiration
-
photorespiratory
-
1,5-bisphosphate
- biliverdin
- bh4
- l-citrulline
- sphingomonas
-
ring-hydroxylating
- comamonas
-
dihydroxylation
- carbazole
- xylene
- l-arg
-
2-oxoglutarate-dependent
-
heme-heme
-
diiron
-
ironii
- 4-methylcatechol
-
monooxygenation
- rubp
- testosteroni
- sphingobium
- ethylbenzene
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4.1.1.39
- degradation
- environmental protection
- fluoranthene
- rhodochrous
Reaction
Synonyms
2,3-dihydroxybenzenesulfonate 2,3-dioxygenase, 3-sulfocatechol-2,3-dioxygenase, 3SC23O, A23O, AtdB, BupB, C2,3O, C23D, C23o, C23O-2G, C23O1, C23O2, C23Os, CatE, catechol 2,3 dioxygenase, catechol 2,3-di-2,3-pyrocatechase, catechol 2,3-dioxygenase, catechol 2,3-oxygenase, catechol oxygenase, catechol-2,3-dioxygenase, CbzE, CD-2,3, EC 1.13.1.2, ECDO, extradiol dioxygenase MhpB, Extradiol-cleaving catecholic dioxygenase, meta-cleavage dioxygenase, metapyrocatechase, More, oxygenase, PheB, pyrocatechol 2,3-dioxygenase, Saci_2295, SSO1223, SsoC2,3O, ssol_2912, tbuE, TdnC, TodE, XylE
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Temperature Stability
Temperature Stability on EC 1.13.11.2 - catechol 2,3-dioxygenase
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30 - 50
4 - 30
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the two isoforms C23O1 and C23O2 are both highly stable in the temperature range of 4-30°C for 24 h. Their activities are kept best at 10°C and the residual activities are about 80% at 24 h, followed by 20°C with approximately 60% residual activies at 24 h. Their enzyme activities decline rapidly as the temperature increases above 50°C
45
50
60
70
85
the structure of thermophilic C23O remains unchanged up to temperatures of 85°C
additional information
the half-life of the enzyme activity at 30°C is about 7 h and the half-life of the enzyme activity at 50°C is 45 min
30 - 50
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38% relative activity of the wild-type enzyme after 1200 min at 30°C, 45% relative activity of mutated enzymes after 1200 min at 30°C, 44% relative activity of wild-type enzyme after 80 min at 50°C, 52% relative activity of mutated enzyme after 80 min at 50°C
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1.5 min, 50% loss of original activity under aerobic conditions
70
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10 min, 50% loss of original activity under nitrogen atmosphere
the observed stability of the enzyme can be explained by the presence of a set of residues surrounding the active center of the enzyme. The solution scattering of the enzyme at temperatures between 4 and 85°C ideally fits the calculated scattering from the single crystal structure
additional information
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the observed stability of the enzyme can be explained by the presence of a set of residues surrounding the active center of the enzyme. The solution scattering of the enzyme at temperatures between 4 and 85°C ideally fits the calculated scattering from the single crystal structure