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1.13.11.2: catechol 2,3-dioxygenase

This is an abbreviated version!
For detailed information about catechol 2,3-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.2

Reaction

catechol
+
O2
=
2-hydroxymuconate-6-semialdehyde

Synonyms

2,3-dihydroxybenzenesulfonate 2,3-dioxygenase, 3-sulfocatechol-2,3-dioxygenase, 3SC23O, A23O, AtdB, BupB, C2,3O, C23D, C23o, C23O-2G, C23O1, C23O2, C23Os, CatE, catechol 2,3 dioxygenase, catechol 2,3-di-2,3-pyrocatechase, catechol 2,3-dioxygenase, catechol 2,3-oxygenase, catechol oxygenase, catechol-2,3-dioxygenase, CbzE, CD-2,3, EC 1.13.1.2, ECDO, extradiol dioxygenase MhpB, Extradiol-cleaving catecholic dioxygenase, meta-cleavage dioxygenase, metapyrocatechase, More, oxygenase, PheB, pyrocatechol 2,3-dioxygenase, Saci_2295, SSO1223, SsoC2,3O, ssol_2912, tbuE, TdnC, TodE, XylE

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.2 catechol 2,3-dioxygenase

Storage Stability

Storage Stability on EC 1.13.11.2 - catechol 2,3-dioxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, 1 week, under nitrogen gas, 72% of the orginal activity
-
-20°C, in lyophilized state for several months, metapyrocatechase 1 and 2
-
-80°C, did not prevent the initial loss of activity
-
0-4°C, 0.02 M Tris-HCl, pH 8.0, fairly stable
-
0°C, 4 h, about 30% loss of activity
-
0°C, 50 mM Tris-acetone buffer, pH 7.5, the inactivation rate with decreasing enzyme concentration
-
22°C, 9 days, crude enzyme is completely inactivated
-
22°C, 9 days, the catalytic activity of the crude enzyme retains 40% of its original enzymatic activity
-
4°C, 10 h, the mutant enzymes lost 95% of activity
-
4°C, 10 mM HEPES, pH 7.5, 150 mM NaCl, aerobic conditions, stable for several weeks
4°C, 3 days, 20% loss of activity of wild-type enzyme
-
4°C, 9 days, the catalytic activity of crude enzyme is reduced to approximately 50% of its original enzymatic activity
-
4°C, 9 days, the catalytic activity of the recombinant Escherichia coli BL21-C23O cells retain 90% of its original enzymatic activity
-
4°C, buffer, containing 5% acetone, pH 6-7,5, 24 h, stable
-
4°C, in acetone buffer under nitrogen for several weeks, metapyrocatechase 1 and 2
-
4°C, over a month, without loss of activity, crystals of holoenzyme in the presence of acetone
-
4°C, the enzyme retains complete activity for more than 350 min when kept under an N2 atmosphere, whereas it loses about 80% of its activity when stored for the same time in the absence of nitrogen
4°C, Tris-acetone buffer, crystalline enzyme was stable for at most six months
-