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Literature summary for 1.13.11.2 extracted from
- The thermophilic archaeon Sulfolobus solfataricus is able to grow on phenol (2005), Res. Microbiol., 156, 677-689.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
catechol | pH 7.5, 25°C | Saccharolobus solfataricus |  |
| 0.0017 | - |
3-methylcatechol | pH 7.5,25 °C | Saccharolobus solfataricus | |
| 0.003 | - |
4-methylcatechol | pH 7.5, 25°C | Saccharolobus solfataricus | |
| 0.0036 | - |
3-methylcatechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
| 0.0059 | - |
catechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
| 0.012 | - |
4-methylcatechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | the enzyme contains about 2 mol of Fe2+ per mole of protein | Saccharolobus solfataricus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| catechol + O2 | Saccharolobus solfataricus | the enzyme is constitutively transcribed | 2-hydroxymuconate-6-semialdehyde | - |
? | |
| catechol + O2 | Saccharolobus solfataricus P2 | the enzyme is constitutively transcribed | 2-hydroxymuconate-6-semialdehyde | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | V5W6K5 | - |
- |
| Saccharolobus solfataricus P2 | V5W6K5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, the enzyme retains complete activity for more than 350 min when kept under an N2 atmosphere, whereas it loses about 80% of its activity when stored for the same time in the absence of nitrogen | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-methylcatechol + O2 | - |
Saccharolobus solfataricus | ? | - |
? | |
| 3-methylcatechol + O2 | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
| 4-methylcatechol + O2 | - |
Saccharolobus solfataricus | ? | - |
? | |
| 4-methylcatechol + O2 | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
| catechol + O2 | - |
Saccharolobus solfataricus | 2-hydroxymuconate-6-semialdehyde | - |
? | |
| catechol + O2 | the enzyme is constitutively transcribed | Saccharolobus solfataricus | 2-hydroxymuconate-6-semialdehyde | - |
? | |
| catechol + O2 | - |
Saccharolobus solfataricus P2 | 2-hydroxymuconate-6-semialdehyde | - |
? | |
| catechol + O2 | the enzyme is constitutively transcribed | Saccharolobus solfataricus P2 | 2-hydroxymuconate-6-semialdehyde | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SSO1223 | locus name | Saccharolobus solfataricus |
| SsoC2,3O | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 80 | - |
Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | 50°C: about 50% of maximal activity, 70-80°C: maximal activity | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life: 230-240 min | Saccharolobus solfataricus |
| 70 | - |
half-life: 100-110 min | Saccharolobus solfataricus |
| 80 | - |
half-life: about 20 min | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.58 | - |
3-methylcatechol | pH 7.5,25 °C | Saccharolobus solfataricus | |
| 0.76 | - |
4-methylcatechol | pH 7.5, 25°C | Saccharolobus solfataricus | |
| 1.3 | - |
catechol | pH 7.5, 25°C | Saccharolobus solfataricus | |
| 4.7 | - |
4-methylcatechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
| 5.2 | - |
3-methylcatechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
| 7.8 | - |
catechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharolobus solfataricus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 250 | - |
4-methylcatechol | pH 7.5, 25°C | Saccharolobus solfataricus | |
| 340 | - |
3-methylcatechol | pH 7.5,25 °C | Saccharolobus solfataricus | |
| 400 | - |
4-methylcatechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
| 810 | - |
catechol | pH 7.5, 25°C | Saccharolobus solfataricus | |
| 1300 | - |
catechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
| 1400 | - |
3-methylcatechol | pH 7.5, 60°C | Saccharolobus solfataricus | |
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